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Literature summary extracted from

  • Helmink, B.A.; Friesen, J.A.
    Characterization of a lipid activated CTP:phosphocholine cytidylyltransferase from Drosophila melanogaster (2004), Biochim. Biophys. Acta, 1683, 78-88.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.15 Lipids regulation of activity by reversible association with membrane lipids, preferentially activated by anionic lipids, highest activation with phosphatidylcholine vesicles containing diphosphatidylglycerol (29fold), less activation with vesicles containing phosphatidylserine (16fold), phosphatidylinositol (21fold), phosphatidylethanolamine (17fold), and oleate (13fold), compared to free enzyme 5fold activation with enzyme associated with pure phosphatidylcholine vesicles Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.15 expressed as native and His-tag fusion protein in Sf9 insect cells, native protein expressed in Escherichia coli but expressed protein was found to be misfolded Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.15 0.5
-
choline phosphate purified native enzyme, pH 7.0, 37°C Drosophila melanogaster
2.7.7.15 0.64
-
choline phosphate purified His-tagged enzyme, pH 7.0, 37°C Drosophila melanogaster
2.7.7.15 0.72
-
CTP purified native enzyme, pH 7.0, 37°C Drosophila melanogaster
2.7.7.15 0.81
-
CTP purified His-tagged enzyme, pH 7.0, 37°C Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.15 membrane enzyme is activated when bound to vesicles Drosophila melanogaster 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.15 44000
-
x * 44000, SDS-PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.15 CTP + choline phosphate Drosophila melanogaster rate limiting enzyme for the synthesis of phosphatidylcholine diphosphate + CDP-choline
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.15 Drosophila melanogaster
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.7.15 phosphoprotein phosphorylated on serine and threonine but not on tyrosine residues Drosophila melanogaster

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.15 native and His-tag protein Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.15 CTP + choline phosphate
-
Drosophila melanogaster diphosphate + CDP-choline
-
?
2.7.7.15 CTP + choline phosphate rate limiting enzyme for the synthesis of phosphatidylcholine Drosophila melanogaster diphosphate + CDP-choline
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.15 ? x * 44000, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
2.7.7.15 CCT2
-
Drosophila melanogaster