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Literature summary extracted from

  • Los, A.P.; van Baal, J.; de Widt, J.; Divecha, N.; van Blitterswijk, W.J.
    Structure-activity relationship of diacylglycerol kinase theta (2004), Biochim. Biophys. Acta, 1636, 169-174.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 expression of GFP-tagged or FLAG-tagged wild-type and mutant isozymes theta in COS-7 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.107 G236R site-directed mutagenesis, highly reduced activity compared to the wild-type isozyme theta Homo sapiens
2.7.1.107 L241V site-directed mutagenesis, slightly reduced activity compared to the wild-type isozyme theta Homo sapiens
2.7.1.107 additional information construction of deletion mutants, N- or C-terminal truncations inactivate the isozyme theta Homo sapiens
2.7.1.107 P244A site-directed mutagenesis, reduced activity compared to the wild-type isozyme theta Homo sapiens
2.7.1.107 P244L site-directed mutagenesis, reduced activity compared to the wild-type isozyme theta Homo sapiens
2.7.1.107 P245L site-directed mutagenesis, highly reduced activity compared to the wild-type isozyme theta Homo sapiens
2.7.1.107 S240T site-directed mutagenesis, activity is unaltered compared to the wild-type isozyme theta Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 RhoA V14-RhoA, strong binding to the C-terminal catalytic domain, negative regulation Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol Homo sapiens
-
 ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Homo sapiens
-
 isozyme theta
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate conserved residues in the extended cysteine-rich domain CRD are essential for activity. e.g. G236, P244, and P245 Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol
-
 Homo sapiens ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 r
2.7.1.107 ATP + 1,2-diacylglycerol 1,2-diacylglycerol is a second messenger Homo sapiens ADP + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?

Subunits

EC Number Subunits Comment Organism
2.7.1.107 More isozyme theta is the only one of 9 isozyme forms, that possesses three instead of two cysteine-rich domains at the N-terminal regulatory region Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.107 DGK
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.107 ATP
-
 Homo sapiens