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Literature summary extracted from

  • Balbo, P.B.; Meinke, G.; Bohm, A.
    Kinetic studies of yeast polyA polymerase indicate an induced fit mechanism for nucleotide specificity (2005), Biochemistry, 44, 7777-7786.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.19 additional information mutation of bovine residue N202 (equivalent to yeast N189) to alanine has essentially no effect on the apparent Km for ATP, but has a pronounced effect on the apparent Vmax, suggesting that this residue is particularly important in the recognition of the adenine of ATP Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.7.7.19 a decay of PAP activity with a half-life of approximately 2 h (on ice) is observed when Nonidet-P 40 is omitted from the enzyme storage solution Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.19 alpha, beta-methylene-ATP a nonreactive ATP analogue Saccharomyces cerevisiae
2.7.7.19 diphosphate product inhibitor Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.19 0.0263
-
 oligo(A)17C incubation at 30 °C for 15 min in the presence of MgATP2- Saccharomyces cerevisiae
2.7.7.19 0.0359
-
 ATP
-
 Saccharomyces cerevisiae
2.7.7.19 0.0468
-
 oligo(A)18 incubation at 30 °C for 15 min in the presence of MgATP2- Saccharomyces cerevisiae
2.7.7.19 0.0642
-
 oligo(A)18 incubation at 30 °C for 15 min in the presence of MgCTP2- Saccharomyces cerevisiae
2.7.7.19 0.1036
-
 CTP
-
 Saccharomyces cerevisiae
2.7.7.19 0.123
-
 ATP incubation at 30 °C for 15 min, using oligo(A)17C (a modification of the 3'-terminal residue from A to C) Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19 recombinant yeast PAP is purified by nickel affinity and anion exchange chromatography using NTA agarose and Source S resins Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + oligo(A)17C
-
 Saccharomyces cerevisiae diphosphate + oligo(A)18C
-
 ?
2.7.7.19 ATP + oligo(A)18
-
 Saccharomyces cerevisiae diphosphate + oligo(A)19
-
 ?
2.7.7.19 ATP + RNAn
-
 Saccharomyces cerevisiae diphosphate + RNAn+1
-
 ?
2.7.7.19 CTP + RNAn
-
 Saccharomyces cerevisiae diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.19 PAP
-
 Saccharomyces cerevisiae
2.7.7.19 polyA polymerase
-
 Saccharomyces cerevisiae
2.7.7.19 polyadenylate polymerase
-
 Saccharomyces cerevisiae