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Literature summary extracted from

  • Alexander, E.T.; Bhat, S.; Thomas, M.J.; Weinberg, R.B.; Cook, V.R.; Bharadwaj, M.S.; Sorci-Thomas, M.
    Apolipoprotein A-I helix 6 negatively charged residues attenuate lecithin-cholesterol acyltransferase (LCAT) reactivity (2005), Biochemistry, 44, 5409-5419.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.43 apolipoprotein A-I principal apolipoprotein of HDL, activates the enzyme, the negative net charge of the protein is important for function in interaction with the enzyme, mutation of the negative charges to uncharged, or mutational doubling of the negatively charged residues, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.43 additional information
-
 additional information
-
 Homo sapiens
2.3.1.43 0.0006
-
 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine pH 7.4, 37°C, recombinant wild-type enzyme with mutant A154D/A158D/T161D/A164D apolipoprotein A-I, HDL with a surface charge of -11.3 mV Homo sapiens
2.3.1.43 0.001
-
 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine pH 7.4, 37°C, recombinant wild-type enzyme with wild-type apolipoprotein A-I, HDL with a surface charge of -8.2 mV Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.43 additional information
-
 activity with 80 and 93 A HDL particles, and with wild-type and different mutant of apolipoprotein A-I, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + cholesterol cholesterol in HDL of 80 and 93 A, the latter gives a 12fold higher activity with recombinant His-tagged enzyme Homo sapiens ?
-
 ?
2.3.1.43 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine + cholesterol
-
 Homo sapiens ?
-
 ?
2.3.1.43 additional information the enzyme activity is largely influenced by HDL particle size and negative net charge of the cofactor apolipoprotein A-I, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.43 LCAT
-
 Homo sapiens
2.3.1.43 lecithin-cholesterol acyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.43 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.43 7.4
-
 assay at Homo sapiens