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Literature summary extracted from

  • Barroso, J.F.; Carvalho, R.N.; Flatmark, T.
    Kinetic analysis and ligand-induced conformational changes in dimeric and tetrameric forms of human thymidine kinase 2 (2005), Biochemistry, 44, 4886-4896.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 DNA and amino acid sequence determination, expression in Escherichia coli as maltose binding fusion protein in inclusion bodies, coexpression of GroEL/ES molecular chaperone system and 3% v/v ethanol supplementation Homo sapiens

General Stability

EC Number General Stability Organism
2.7.1.21 binding of dNTP inhibitors and substrates, i.e. 2'-deoxycytidine, Mg-2'-deoxyCTP, 2'-deoxythymidine, and Mg-2'-deoxyTTP structurally stabilizes the enzyme, ligand-bound enzyme is less susceptible to aggregation, proteolysis by trypsin, and thermal denaturation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 dATP strong feedback inhibition, tight binding at the active site in stoichiometric amount Homo sapiens
2.7.1.21 dCTP strong feedback inhibition, tight binding at the active site in stoichiometric amount Homo sapiens
2.7.1.21 dTTP strong feedback inhibition, tight binding at the active site in stoichiometric amount Homo sapiens
2.7.1.21 additional information inhibition potency of dNTPs in descending order: dTTP, dCTP, dATP, inhibitor binding structurally stabilizes the enzyme, induction of conformational changes upon ligand binding, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 additional information
-
 additional information steady-state kinetics, kinetics of conformational changes induced by ligand binding Homo sapiens
2.7.1.21 0.0028
-
 ATP dNTP-free recombinant dimeric enzyme, pH 7.5, 25°C Homo sapiens
2.7.1.21 0.0028
-
 ATP ATP in form of MgATP2- Homo sapiens
2.7.1.21 0.004
-
 2'-deoxycytidine dNTP-free recombinant dimeric enzyme, pH 7.5, 25°C Homo sapiens
2.7.1.21 0.0049
-
 2'-deoxycytidine dNTP-free recombinant tetrameric enzyme, pH 7.5, 25°C Homo sapiens
2.7.1.21 0.0058
-
 2'-deoxythymidine dNTP-free recombinant dimeric enzyme, pH 7.5, 25°C Homo sapiens
2.7.1.21 0.0059
-
 ATP dNTP-free recombinant tetrameric enzyme, pH 7.5, 25°C Homo sapiens
2.7.1.21 0.0059
-
 ATP ATP in form of MgATP2- Homo sapiens
2.7.1.21 0.0065
-
 2'-deoxythymidine dNTP-free recombinant tetrameric enzyme, pH 7.5, 25°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.21 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.21 ATP + 2'-deoxycytidine Homo sapiens
-
 ADP + 2'-deoxycytidine 5'-phosphate
-
 ir
2.7.1.21 ATP + 2'-deoxythymidine Homo sapiens
-
 ADP + 2'-deoxythymidine 5'-phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 recombinant enzyme as maltose binding fusion protein from Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ir
2.7.1.21 ATP + 2'-deoxycytidine induction of conformational changes upon ligand binding, overview Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ir
2.7.1.21 ATP + 2'-deoxythymidine
-
 Homo sapiens ADP + 2'-deoxythymidine 5'-phosphate
-
 ir
2.7.1.21 ATP + 2'-deoxythymidine induction of conformational changes upon ligand binding, overview Homo sapiens ADP + 2'-deoxythymidine 5'-phosphate
-
 ir
2.7.1.21 ATP + thymidine
-
 Homo sapiens ADP + dTMP
-
 ?
2.7.1.21 additional information ligand-induced conformational changes in dimeric and tetrameric forms of the enzyme alter the catalytic activity Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 dimer the most abundant enzyme form, less active than the tetramer Homo sapiens
2.7.1.21 More ligand-induced conformational changes in dimeric and tetrameric forms of the enzyme Homo sapiens
2.7.1.21 tetramer less abundant form, more active than the dimer Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.21 More the enzyme belongs to the thymidine kinase 2, i.e. TK2, family of enzymes Homo sapiens
2.7.1.21 thymidine kinase 2
-
 Homo sapiens
2.7.1.21 TK2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.21 25
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.21 additional information
-
 irreversible thermal denaturation following a two-state irrversible denaturation model, study with ligand-bound and free enzyme, respectively, overview Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.21 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.21 MgATP2-
-
 Homo sapiens