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Literature summary extracted from
- Chlorella virus Marburg topoisomerase II: high DNA cleavage activity as a characteristic of Chlorella virus type II enzymes (2005), Biochemistry, 44, 3899-3908.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.2 | amsacrine | weak activation of DNA cleavage at some sites | Chlorella virus Marburg |  |
| 5.6.2.2 | etoposide | weak activation of DNA cleavage at some sites | Chlorella virus Marburg | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.6.2.2 | expression in Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Chlorella virus Marburg |
| 5.6.2.2 | expression in Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Paramecium bursaria chlorella virus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.2 | amsacrine | weak inhibition of DNA cleavage at some sites, primarily inhibition of DNA ligation, low sensitivity for the drug | Chlorella virus Marburg | |
| 5.6.2.2 | CP-115953 | - |
Chlorella virus Marburg | |
| 5.6.2.2 | EDTA | - |
Chlorella virus Marburg | |
| 5.6.2.2 | EDTA | - |
Paramecium bursaria chlorella virus | |
| 5.6.2.2 | etoposide | weak inhibition of DNA cleavage at some sites, primarily inhibition of DNA ligation, low sensitivity for the drug | Chlorella virus Marburg | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.2 | KCl | - |
Chlorella virus Marburg | |
| 5.6.2.2 | KCl | - |
Paramecium bursaria chlorella virus | |
| 5.6.2.2 | Mg2+ | required for cleavage | Chlorella virus Marburg | |
| 5.6.2.2 | Mg2+ | required for cleavage | Paramecium bursaria chlorella virus | |
| 5.6.2.2 | NaCl | - |
Paramecium bursaria chlorella virus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.2 | dsDNA + ATP + H2O | Paramecium bursaria chlorella virus | breakage, passage, decatenation, and rejoining of dsDNA, enzyme is required for conrol of DNA topology | ? | - |
? | |
| 5.6.2.2 | dsDNA + ATP + H2O | Chlorella virus Marburg | breakage, passage, decatenation, and rejoining of dsDNA, enzyme is required for control of DNA topology | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.2.2 | Chlorella virus Marburg | - |
i.e. CVM-1 | - |
| 5.6.2.2 | Paramecium bursaria chlorella virus | - |
i.e. PBCV-1 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.2 | dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, enzyme is required for conrol of DNA topology | Paramecium bursaria chlorella virus | ? | - |
? | |
| 5.6.2.2 | dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, enzyme is required for control of DNA topology | Chlorella virus Marburg | ? | - |
? | |
| 5.6.2.2 | dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity and high strand passage activity of the viral type enzyme, tight DNA binding, mapping of DNA cleavage sites | Chlorella virus Marburg | ? | - |
? | |
| 5.6.2.2 | dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme, mapping of DNA cleavage sites | Paramecium bursaria chlorella virus | ? | - |
? | |
| 5.6.2.2 | kinetoplast DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of kinetoplast DNA, kinetoplast DNA is isolated from Crithidia fasciculata | Chlorella virus Marburg | ? | - |
? | |
| 5.6.2.2 | additional information | formation of a covalent enzyme-DNA complex is a prerequisite for activity, the intermediate can destabilize the genome | Chlorella virus Marburg | ? | - |
? | |
| 5.6.2.2 | additional information | formation of a covalent enzyme-DNA complex is a prerequisite for activity, the intermediate can destabilize the genome | Paramecium bursaria chlorella virus | ? | - |
? | |
| 5.6.2.2 | negatively supercoiled pBR322 DNA + ATP + H2O | breakage, passage, and rejoining of negatively supercoiled pBR322 DNA | Paramecium bursaria chlorella virus | ? | - |
? | |
| 5.6.2.2 | negatively supercoiled pBR322 DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of negatively supercoiled pBR322 DNA | Chlorella virus Marburg | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.6.2.2 | Topoisomerase II | - |
Chlorella virus Marburg |
| 5.6.2.2 | Topoisomerase II | - |
Paramecium bursaria chlorella virus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.2 | 25 | - |
assay at | Paramecium bursaria chlorella virus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.2 | 30 | - |
assay at | Chlorella virus Marburg |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.2 | 8.5 | - |
assay at | Chlorella virus Marburg |
| 5.6.2.2 | 8.5 | - |
assay at | Paramecium bursaria chlorella virus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.2 | ATP | dependent on | Paramecium bursaria chlorella virus | |
| 5.6.2.2 | ATP | dependent on, required for DNA relaxation and decatenation | Chlorella virus Marburg | |
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