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Literature summary extracted from

  • Guth, E.; Connolly, S.H.; Bovee, M.; Francklyn, C.S.
    A substrate-assisted concerted mechanism for aminoacylation by a class II aminoacyl-tRNA synthetase (2005), Biochemistry, 44, 3785-3794.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.21 E83A 15fold increase in Km-value, decrease in kcat-value Escherichia coli
6.1.1.21 E83Q 2fold decrease in Km-value, decrease in kcat-value Escherichia coli
6.1.1.21 Q127A decrease in kcat-value Escherichia coli
6.1.1.21 R259H 10fold increase in Km-value, decrease in kcat-value Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.21 0.00017
-
 tRNAHis mutant E83Q, pH 7.5, 37°C Escherichia coli
6.1.1.21 0.00034
-
 tRNAHis wild-type, pH 7.5, 37°C Escherichia coli
6.1.1.21 0.00048
-
 tRNAHis mutant Q127A, pH 7.5, 37°C Escherichia coli
6.1.1.21 0.0033
-
 tRNAHis mutant R159H, pH 7.5, 37°C Escherichia coli
6.1.1.21 0.00515
-
 tRNAHis mutant E83A, pH 7.5, 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Escherichia coli P60906
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.21 ATP + L-histidine + tRNAHis = AMP + diphosphate + L-histidyl-tRNAHis substrate-assisted concerted mechanism, rate of aminoacyl transfer is independent of the interaction between the carboxamide group of Q127 and the alpha-carboxylate carbon Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Escherichia coli AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.21 0.0004
-
 tRNAHis mutant E83A, pH 7.5, 37°C Escherichia coli
6.1.1.21 0.0006
-
 tRNAHis mutant R159H, pH 7.5, 37°C Escherichia coli
6.1.1.21 0.01
-
 tRNAHis mutant E83Q, pH 7.5, 37°C Escherichia coli
6.1.1.21 0.048
-
 tRNAHis mutant Q127A, pH 7.5, 37°C Escherichia coli
6.1.1.21 2.02
-
 tRNAHis wild-type, pH 7.5, 37°C Escherichia coli