EC Number | Cloned (Comment) | Organism |
---|---|---|
5.6.2.2 | expression in Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Paramecium bursaria Chlorella virus 1 |
5.6.2.2 | expression in Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Chlorella virus Marburg |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.6.2.2 | additional information | 5-methylcytosine in DNA has a weak effect on enzyme DNA scission activity, 5mC has a 42% content in viral DNA | Chlorella virus Marburg | |
5.6.2.2 | additional information | 5-methylcytosine in DNA has a weak effect on enzyme DNA scission activity, 5mC has a high content in viral DNA | Paramecium bursaria Chlorella virus 1 | |
5.6.2.2 | N6-Methyladenine | i.e.6 mA, inhibits DNA scission by the enzyme, 10% content in viral DNA | Chlorella virus Marburg | |
5.6.2.2 | N6-Methyladenine | i.e.6mA, inhibits DNA scission by the enzyme, high content in viral DNA | Paramecium bursaria Chlorella virus 1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.6.2.2 | additional information | - |
additional information | DNA cleavage kinetics | Paramecium bursaria Chlorella virus 1 | |
5.6.2.2 | additional information | - |
additional information | DNA cleavage kinetics | Chlorella virus Marburg |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.6.2.2 | KCl | - |
Paramecium bursaria Chlorella virus 1 | |
5.6.2.2 | KCl | - |
Chlorella virus Marburg | |
5.6.2.2 | Mg2+ | required for cleavage | Paramecium bursaria Chlorella virus 1 | |
5.6.2.2 | Mg2+ | required for cleavage | Chlorella virus Marburg | |
5.6.2.2 | NaCl | - |
Paramecium bursaria Chlorella virus 1 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.2.2 | dsDNA + ATP + H2O | Paramecium bursaria Chlorella virus 1 | breakage, passage, decatenation, and rejoining of dsDNA | ? | - |
? | |
5.6.2.2 | dsDNA + ATP + H2O | Chlorella virus Marburg | breakage, passage, decatenation, and rejoining of dsDNA | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.6.2.2 | Chlorella virus Marburg | - |
i.e. CVM-1 | - |
5.6.2.2 | Paramecium bursaria Chlorella virus 1 | - |
i.e. PBCV-1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.6.2.2 | recombinant viral enzyme from Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Paramecium bursaria Chlorella virus 1 |
5.6.2.2 | recombinant viral enzyme from Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Chlorella virus Marburg |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.2.2 | CVM-1 viral DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of CVM-1 viral DNA, viral DNA contains 10% N6-methyladenine and 42% 5-methylcytosine, 4fold decreased activity compared to unmodified DNA | Chlorella virus Marburg | ? | - |
? | |
5.6.2.2 | dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA | Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
5.6.2.2 | dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA | Chlorella virus Marburg | ? | - |
? | |
5.6.2.2 | dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme | Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
5.6.2.2 | dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme | Chlorella virus Marburg | ? | - |
? | |
5.6.2.2 | additional information | the enzyme binds unmodified DNA and DNA containing N6-methyladenine and 5-methylcytosine to the same extent | Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
5.6.2.2 | additional information | the enzyme binds unmodified DNA and DNA containing N6-methyladenine and 5-methylcytosine to the same extent | Chlorella virus Marburg | ? | - |
? | |
5.6.2.2 | PBCV-1 viral DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of PBCV-1 viral DNA, viral DNA contains N6-methyladenine and 5-methylcytosine, decreased activity compared to unmodified DNA | Paramecium bursaria Chlorella virus 1 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.6.2.2 | Topoisomerase II | - |
Paramecium bursaria Chlorella virus 1 |
5.6.2.2 | Topoisomerase II | - |
Chlorella virus Marburg |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.6.2.2 | 25 | - |
assay at | Paramecium bursaria Chlorella virus 1 |
5.6.2.2 | 30 | - |
assay at | Chlorella virus Marburg |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.6.2.2 | 8.5 | - |
assay at | Paramecium bursaria Chlorella virus 1 |
5.6.2.2 | 8.5 | - |
assay at | Chlorella virus Marburg |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.6.2.2 | ATP | - |
Paramecium bursaria Chlorella virus 1 | |
5.6.2.2 | ATP | - |
Chlorella virus Marburg |