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Literature summary extracted from

  • Dickey, J.S.; Van Eantten, J.L.; Osheroff, N.
    DNA methylation impacts the cleavage activity of Chlorella virus topoisomerase II (2005), Biochemistry, 44, 15378-15386.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.2 expression in Saccharomyces cerevisiae strain JEL-1DELTAtop1 Paramecium bursaria Chlorella virus 1
5.6.2.2 expression in Saccharomyces cerevisiae strain JEL-1DELTAtop1 Chlorella virus Marburg

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.2 additional information 5-methylcytosine in DNA has a weak effect on enzyme DNA scission activity, 5mC has a 42% content in viral DNA Chlorella virus Marburg
5.6.2.2 additional information 5-methylcytosine in DNA has a weak effect on enzyme DNA scission activity, 5mC has a high content in viral DNA Paramecium bursaria Chlorella virus 1
5.6.2.2 N6-Methyladenine i.e.6 mA, inhibits DNA scission by the enzyme, 10% content in viral DNA Chlorella virus Marburg
5.6.2.2 N6-Methyladenine i.e.6mA, inhibits DNA scission by the enzyme, high content in viral DNA Paramecium bursaria Chlorella virus 1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6.2.2 additional information
-
additional information DNA cleavage kinetics Paramecium bursaria Chlorella virus 1
5.6.2.2 additional information
-
additional information DNA cleavage kinetics Chlorella virus Marburg

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.2 KCl
-
Paramecium bursaria Chlorella virus 1
5.6.2.2 KCl
-
Chlorella virus Marburg
5.6.2.2 Mg2+ required for cleavage Paramecium bursaria Chlorella virus 1
5.6.2.2 Mg2+ required for cleavage Chlorella virus Marburg
5.6.2.2 NaCl
-
Paramecium bursaria Chlorella virus 1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.2.2 dsDNA + ATP + H2O Paramecium bursaria Chlorella virus 1 breakage, passage, decatenation, and rejoining of dsDNA ?
-
?
5.6.2.2 dsDNA + ATP + H2O Chlorella virus Marburg breakage, passage, decatenation, and rejoining of dsDNA ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Chlorella virus Marburg
-
i.e. CVM-1
-
5.6.2.2 Paramecium bursaria Chlorella virus 1
-
i.e. PBCV-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.2 recombinant viral enzyme from Saccharomyces cerevisiae strain JEL-1DELTAtop1 Paramecium bursaria Chlorella virus 1
5.6.2.2 recombinant viral enzyme from Saccharomyces cerevisiae strain JEL-1DELTAtop1 Chlorella virus Marburg

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 CVM-1 viral DNA + ATP + H2O breakage, passage, decatenation, and rejoining of CVM-1 viral DNA, viral DNA contains 10% N6-methyladenine and 42% 5-methylcytosine, 4fold decreased activity compared to unmodified DNA Chlorella virus Marburg ?
-
?
5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA Paramecium bursaria Chlorella virus 1 ?
-
?
5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA Chlorella virus Marburg ?
-
?
5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme Paramecium bursaria Chlorella virus 1 ?
-
?
5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme Chlorella virus Marburg ?
-
?
5.6.2.2 additional information the enzyme binds unmodified DNA and DNA containing N6-methyladenine and 5-methylcytosine to the same extent Paramecium bursaria Chlorella virus 1 ?
-
?
5.6.2.2 additional information the enzyme binds unmodified DNA and DNA containing N6-methyladenine and 5-methylcytosine to the same extent Chlorella virus Marburg ?
-
?
5.6.2.2 PBCV-1 viral DNA + ATP + H2O breakage, passage, decatenation, and rejoining of PBCV-1 viral DNA, viral DNA contains N6-methyladenine and 5-methylcytosine, decreased activity compared to unmodified DNA Paramecium bursaria Chlorella virus 1 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
5.6.2.2 Topoisomerase II
-
Paramecium bursaria Chlorella virus 1
5.6.2.2 Topoisomerase II
-
Chlorella virus Marburg

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.2.2 25
-
assay at Paramecium bursaria Chlorella virus 1
5.6.2.2 30
-
assay at Chlorella virus Marburg

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.2 8.5
-
assay at Paramecium bursaria Chlorella virus 1
5.6.2.2 8.5
-
assay at Chlorella virus Marburg

Cofactor

EC Number Cofactor Comment Organism Structure
5.6.2.2 ATP
-
Paramecium bursaria Chlorella virus 1
5.6.2.2 ATP
-
Chlorella virus Marburg