BRENDA - Enzyme Database show

Lipid sulfates and sulfonates are allosteric competitive inhibitors of the N-terminal phosphatase activity of the mammalian soluble poxide hydrolase

Tran, K.L.; Aronov, P.A.; Tanaka, H.; Newman, J.W.; Hammock, B.D.; Morisseau, C.; Biochemistry 44, 12179-12187 (2005)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.76
(9E)-12-sulfonooxy-9-octadecenoic acid
IC50 is 0.0097 mM
Homo sapiens
3.1.3.76
(9Z)-12-sulfonooxy-9-octadecenoic acid
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
10-(sulfooxy)octadecanoic acid
IC50 is 0.0059 mM
Homo sapiens
3.1.3.76
4-nitrophenyl sulfate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
9-hydroxy-10-(sulfooxy)octadecanoic acid
IC50 is 0.0175 mM
Homo sapiens
3.1.3.76
alpha-hydroxyfarnesyl phosphonic acid
IC50 is 0.073 mM
Homo sapiens
3.1.3.76
alpha-sulfostearic acid
IC50 is 0.0096 mM
Homo sapiens
3.1.3.76
D-galactose 6-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
dibenzyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl 2,2,2-trifluoro-1-hydroxyethyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl 4-methylbenzyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl allyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl benzoylphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl cyclopropyl methylphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl ethylthiomethyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl trans-cinnamyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl vinylphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
dimethyl 2-oxoheptyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
dioctyl phenyl phosphonate
IC50 is 0.013 mM
Homo sapiens
3.1.3.76
dodecyl phosphonic acid
IC50 is 0.040 mM
Homo sapiens
3.1.3.76
Estrone 3-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
L-ascorbic acid 2-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
additional information
inhibition mechanism of sulfates, sulfonates, and phosphonates
Homo sapiens
3.1.3.76
N-acetyl-D-galactosamine 4-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
octadecan-9-yl sulfate
IC50 is 0.0047 mM
Homo sapiens
3.1.3.76
Sodium dodecyl sulfate
IC50 is 0.0052 mM
Homo sapiens
3.1.3.76
sodium dodecyl sulfonate
IC50 is 0.0037 mM
Homo sapiens
3.1.3.76
taurocholic acid
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
taurolithocholic acid 3-sulfate
IC50 is 0.005 mM
Homo sapiens
3.1.3.76
tetraisopropyl methylenediphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
10-(sulfooxy)octadecanoic acid
IC50 is 0.028 mM
Homo sapiens
3.3.2.10
12-sulfonoxy-cis-9-octadecenoic acid
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
12-sulfonoxy-trans-9-octadecenoic acid
IC50 is 0.016 mM
Homo sapiens
3.3.2.10
4-nitrophenyl sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
9-hydroxy-10-(sulfooxy)octadecanoic acid
IC50 is 0.09 mM
Homo sapiens
3.3.2.10
alpha-hydroxyfarnesyl phosphonic acid
IC50 is 0.073 mM
Homo sapiens
3.3.2.10
alpha-sulfostearic acid
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
D-galactose 6-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
dibenzyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl 2,2,2-trifluoro-1-hydroxyethyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl 4-methylbenzyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl allyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl benzoylphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl cyclopropyl methylphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl ethylthiomethyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl trans-cinnamyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl vinylphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
dimethyl 2-oxoheptyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
dioctyl phenyl phosphonate
IC50 is 0.092 mM
Homo sapiens
3.3.2.10
dodecyl phosphonic acid
IC50 is 0.040 mM
Homo sapiens
3.3.2.10
Estrone 3-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
L-ascorbic acid 2-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
additional information
inhibition mechanism of sulfates, sulfonates, and phosphonates
Homo sapiens
3.3.2.10
N-acetyl-D-galactosamine 4-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
octadecan-9-yl sulfate
IC50 is 0.021 mM
Homo sapiens
3.3.2.10
Sodium dodecyl sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
sodium dodecyl sulfonate
IC50 is 0.05 mM
Homo sapiens
3.3.2.10
taurocholic acid
IC50 is 0.09 mM
Homo sapiens
3.3.2.10
taurolithocholic acid 3-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
tetraisopropyl methylenediphosphonate
IC50 is above 0.1 mM
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.76
additional information
-
additional information
kinetics
Homo sapiens
3.1.3.76
0.0036
-
2'-[2-benzothiazolyl]-6'-hydroxybenzothiazole phosphate
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
3.1.3.76
0.0036
-
farnesyl diphosphate
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
3.1.3.76
0.0036
-
farnesyl monophosphate
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
3.1.3.76
0.0036
-
geranylgeranyl diphosphate
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
3.1.3.76
0.021
-
threo-9,10-phosphonooxyoctadecanoic acid
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
3.1.3.76
0.21
-
4-methylumbelliferyl phosphate
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
3.1.3.76
1.6
-
4-nitrophenyl phosphate
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.76
Mg2+
dependent on
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.76
Homo sapiens
-
-
-
3.3.2.10
Homo sapiens
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
3.1.3.76
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate
the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently, reaction mechanism
Homo sapiens
3.3.2.10
an epoxide + H2O = a glycol
the C-terminal part harbors the epoxide hydrolase activity, the phosphatase activity of the enzyme is located at the N-terminal part of EC 3.1.3.76, both catalytic sites act independently
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.76
(9E)-12-sulfonooxyoctadec-9-enoic acid + H2O
-
661168
Homo sapiens
(9E)-12-hydroxyoctadec-9-enoic acid + sulfate
-
-
-
?
3.1.3.76
(9Z)-12-sulfonooxyoctadec-9-enoic acid + H2O
-
661168
Homo sapiens
(9Z)-12-hydroxyoctadec-9-enoic acid + sulfate
-
-
-
?
3.1.3.76
10-sulfonooxy-octadecanoic acid + H2O
-
661168
Homo sapiens
10-hydroxyoctadecanoic acid + phosphate
-
-
-
?
3.1.3.76
2'-[2-benzothiazolyl]-6'-hydroxybenzothiazole phosphate + H2O
i.e. attophos
661168
Homo sapiens
2'-[2-benzothiazolyl]-6'-hydroxybenzothiazole + phosphate
-
-
-
?
3.1.3.76
4-methylumbelliferyl phosphate + H2O
-
661168
Homo sapiens
4-methylumbelliferone + phosphate
-
-
-
?
3.1.3.76
4-nitrophenyl phosphate + H2O
-
661168
Homo sapiens
4-nitrophenol + phosphate
-
-
-
?
3.1.3.76
farnesyl diphosphate + H2O
-
661168
Homo sapiens
farnesol + phosphate
-
-
-
?
3.1.3.76
farnesyl phosphate + H2O
-
661168
Homo sapiens
farnesol + phosphate
-
-
-
?
3.1.3.76
geranyl diphosphate + H2O
-
661168
Homo sapiens
geraniol + phosphate
-
-
-
?
3.1.3.76
geranylgeranyl diphosphate + H2O
-
661168
Homo sapiens
geranylgeraniol + phosphate
-
-
-
?
3.1.3.76
threo-9,10-phosphonooxyoctadecanoic acid + H2O
-
661168
Homo sapiens
?
-
-
-
?
3.3.2.10
4-nitrophenyl-trans-2,3-epoxy-3-phenylpropyl carbonate + H2O
-
661168
Homo sapiens
?
-
-
-
?
3.3.2.10
racemic trans-1,3-diphenylpropene oxide + H2O
-
661168
Homo sapiens
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.76
More
the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.76
30
-
assay at
Homo sapiens
3.3.2.10
30
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.76
7
-
assay at
Homo sapiens
3.3.2.10
7.4
-
assay at
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.3.76
additional information
-
additional information
inhibition kinetics
Homo sapiens
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.3.76
0.0037
-
IC50 is 0.0037 mM
Homo sapiens
sodium dodecyl sulfonate
3.1.3.76
0.0047
-
IC50 is 0.0047 mM
Homo sapiens
octadecan-9-yl sulfate
3.1.3.76
0.005
-
IC50 is 0.005 mM
Homo sapiens
taurolithocholic acid 3-sulfate
3.1.3.76
0.0052
-
IC50 is 0.0052 mM
Homo sapiens
Sodium dodecyl sulfate
3.1.3.76
0.0059
-
IC50 is 0.0059 mM
Homo sapiens
10-(sulfooxy)octadecanoic acid
3.1.3.76
0.0096
-
IC50 is 0.0096 mM
Homo sapiens
alpha-sulfostearic acid
3.1.3.76
0.0097
-
IC50 is 0.0097 mM
Homo sapiens
(9E)-12-sulfonooxy-9-octadecenoic acid
3.1.3.76
0.013
-
IC50 is 0.013 mM
Homo sapiens
dioctyl phenyl phosphonate
3.1.3.76
0.0175
-
IC50 is 0.0175 mM
Homo sapiens
9-hydroxy-10-(sulfooxy)octadecanoic acid
3.1.3.76
0.04
-
IC50 is 0.040 mM
Homo sapiens
dodecyl phosphonic acid
3.1.3.76
0.073
-
IC50 is 0.073 mM
Homo sapiens
alpha-hydroxyfarnesyl phosphonic acid
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
4-nitrophenyl sulfate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
(9Z)-12-sulfonooxy-9-octadecenoic acid
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
D-galactose 6-sulfate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
dibenzyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl 2,2,2-trifluoro-1-hydroxyethyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl 4-methylbenzyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl allyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl benzoylphosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl cyclopropyl methylphosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl ethylthiomethyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl trans-cinnamyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl vinylphosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
dimethyl 2-oxoheptyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
Estrone 3-sulfate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
L-ascorbic acid 2-sulfate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
N-acetyl-D-galactosamine 4-sulfate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
taurocholic acid
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
tetraisopropyl methylenediphosphonate
3.3.2.10
0.016
-
IC50 is 0.016 mM
Homo sapiens
12-sulfonoxy-trans-9-octadecenoic acid
3.3.2.10
0.021
-
IC50 is 0.021 mM
Homo sapiens
octadecan-9-yl sulfate
3.3.2.10
0.028
-
IC50 is 0.028 mM
Homo sapiens
10-(sulfooxy)octadecanoic acid
3.3.2.10
0.04
-
IC50 is 0.040 mM
Homo sapiens
dodecyl phosphonic acid
3.3.2.10
0.05
-
IC50 is 0.05 mM
Homo sapiens
sodium dodecyl sulfonate
3.3.2.10
0.073
-
IC50 is 0.073 mM
Homo sapiens
alpha-hydroxyfarnesyl phosphonic acid
3.3.2.10
0.09
-
IC50 is 0.09 mM
Homo sapiens
9-hydroxy-10-(sulfooxy)octadecanoic acid
3.3.2.10
0.09
-
IC50 is 0.09 mM
Homo sapiens
taurocholic acid
3.3.2.10
0.092
-
IC50 is 0.092 mM
Homo sapiens
dioctyl phenyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
12-sulfonoxy-cis-9-octadecenoic acid
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
4-nitrophenyl sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
alpha-sulfostearic acid
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
D-galactose 6-sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
dibenzyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl 2,2,2-trifluoro-1-hydroxyethyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl 4-methylbenzyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl allyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl benzoylphosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl cyclopropyl methylphosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl ethylthiomethyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl trans-cinnamyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl vinylphosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
dimethyl 2-oxoheptyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
Estrone 3-sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
L-ascorbic acid 2-sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
N-acetyl-D-galactosamine 4-sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
Sodium dodecyl sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
taurolithocholic acid 3-sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
tetraisopropyl methylenediphosphonate
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.3.76
0.0037
-
IC50 is 0.0037 mM
Homo sapiens
sodium dodecyl sulfonate
3.1.3.76
0.0047
-
IC50 is 0.0047 mM
Homo sapiens
octadecan-9-yl sulfate
3.1.3.76
0.005
-
IC50 is 0.005 mM
Homo sapiens
taurolithocholic acid 3-sulfate
3.1.3.76
0.0052
-
IC50 is 0.0052 mM
Homo sapiens
Sodium dodecyl sulfate
3.1.3.76
0.0059
-
IC50 is 0.0059 mM
Homo sapiens
10-(sulfooxy)octadecanoic acid
3.1.3.76
0.0096
-
IC50 is 0.0096 mM
Homo sapiens
alpha-sulfostearic acid
3.1.3.76
0.0097
-
IC50 is 0.0097 mM
Homo sapiens
(9E)-12-sulfonooxy-9-octadecenoic acid
3.1.3.76
0.013
-
IC50 is 0.013 mM
Homo sapiens
dioctyl phenyl phosphonate
3.1.3.76
0.0175
-
IC50 is 0.0175 mM
Homo sapiens
9-hydroxy-10-(sulfooxy)octadecanoic acid
3.1.3.76
0.04
-
IC50 is 0.040 mM
Homo sapiens
dodecyl phosphonic acid
3.1.3.76
0.073
-
IC50 is 0.073 mM
Homo sapiens
alpha-hydroxyfarnesyl phosphonic acid
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
4-nitrophenyl sulfate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
(9Z)-12-sulfonooxy-9-octadecenoic acid
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
D-galactose 6-sulfate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
dibenzyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl 2,2,2-trifluoro-1-hydroxyethyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl 4-methylbenzyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl allyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl benzoylphosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl cyclopropyl methylphosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl ethylthiomethyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl trans-cinnamyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl vinylphosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
dimethyl 2-oxoheptyl phosphonate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
Estrone 3-sulfate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
L-ascorbic acid 2-sulfate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
N-acetyl-D-galactosamine 4-sulfate
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
taurocholic acid
3.1.3.76
0.1
-
IC50 is above 0.1 mM
Homo sapiens
tetraisopropyl methylenediphosphonate
3.3.2.10
0.016
-
IC50 is 0.016 mM
Homo sapiens
12-sulfonoxy-trans-9-octadecenoic acid
3.3.2.10
0.021
-
IC50 is 0.021 mM
Homo sapiens
octadecan-9-yl sulfate
3.3.2.10
0.028
-
IC50 is 0.028 mM
Homo sapiens
10-(sulfooxy)octadecanoic acid
3.3.2.10
0.04
-
IC50 is 0.040 mM
Homo sapiens
dodecyl phosphonic acid
3.3.2.10
0.05
-
IC50 is 0.05 mM
Homo sapiens
sodium dodecyl sulfonate
3.3.2.10
0.073
-
IC50 is 0.073 mM
Homo sapiens
alpha-hydroxyfarnesyl phosphonic acid
3.3.2.10
0.09
-
IC50 is 0.09 mM
Homo sapiens
9-hydroxy-10-(sulfooxy)octadecanoic acid
3.3.2.10
0.09
-
IC50 is 0.09 mM
Homo sapiens
taurocholic acid
3.3.2.10
0.092
-
IC50 is 0.092 mM
Homo sapiens
dioctyl phenyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
12-sulfonoxy-cis-9-octadecenoic acid
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
4-nitrophenyl sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
alpha-sulfostearic acid
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
D-galactose 6-sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
dibenzyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl 2,2,2-trifluoro-1-hydroxyethyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl 4-methylbenzyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl allyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl benzoylphosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl cyclopropyl methylphosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl ethylthiomethyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl trans-cinnamyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
diethyl vinylphosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
dimethyl 2-oxoheptyl phosphonate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
Estrone 3-sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
L-ascorbic acid 2-sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
N-acetyl-D-galactosamine 4-sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
Sodium dodecyl sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
taurolithocholic acid 3-sulfate
3.3.2.10
0.1
-
IC50 is above 0.1 mM
Homo sapiens
tetraisopropyl methylenediphosphonate
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.76
(9E)-12-sulfonooxy-9-octadecenoic acid
IC50 is 0.0097 mM
Homo sapiens
3.1.3.76
(9Z)-12-sulfonooxy-9-octadecenoic acid
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
10-(sulfooxy)octadecanoic acid
IC50 is 0.0059 mM
Homo sapiens
3.1.3.76
4-nitrophenyl sulfate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
9-hydroxy-10-(sulfooxy)octadecanoic acid
IC50 is 0.0175 mM
Homo sapiens
3.1.3.76
alpha-hydroxyfarnesyl phosphonic acid
IC50 is 0.073 mM
Homo sapiens
3.1.3.76
alpha-sulfostearic acid
IC50 is 0.0096 mM
Homo sapiens
3.1.3.76
D-galactose 6-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
dibenzyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl 2,2,2-trifluoro-1-hydroxyethyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl 4-methylbenzyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl allyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl benzoylphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl cyclopropyl methylphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl ethylthiomethyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl trans-cinnamyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
diethyl vinylphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
dimethyl 2-oxoheptyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
dioctyl phenyl phosphonate
IC50 is 0.013 mM
Homo sapiens
3.1.3.76
dodecyl phosphonic acid
IC50 is 0.040 mM
Homo sapiens
3.1.3.76
Estrone 3-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
L-ascorbic acid 2-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
additional information
inhibition mechanism of sulfates, sulfonates, and phosphonates
Homo sapiens
3.1.3.76
N-acetyl-D-galactosamine 4-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
octadecan-9-yl sulfate
IC50 is 0.0047 mM
Homo sapiens
3.1.3.76
Sodium dodecyl sulfate
IC50 is 0.0052 mM
Homo sapiens
3.1.3.76
sodium dodecyl sulfonate
IC50 is 0.0037 mM
Homo sapiens
3.1.3.76
taurocholic acid
IC50 is above 0.1 mM
Homo sapiens
3.1.3.76
taurolithocholic acid 3-sulfate
IC50 is 0.005 mM
Homo sapiens
3.1.3.76
tetraisopropyl methylenediphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
10-(sulfooxy)octadecanoic acid
IC50 is 0.028 mM
Homo sapiens
3.3.2.10
12-sulfonoxy-cis-9-octadecenoic acid
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
12-sulfonoxy-trans-9-octadecenoic acid
IC50 is 0.016 mM
Homo sapiens
3.3.2.10
4-nitrophenyl sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
9-hydroxy-10-(sulfooxy)octadecanoic acid
IC50 is 0.09 mM
Homo sapiens
3.3.2.10
alpha-hydroxyfarnesyl phosphonic acid
IC50 is 0.073 mM
Homo sapiens
3.3.2.10
alpha-sulfostearic acid
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
D-galactose 6-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
dibenzyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl 2,2,2-trifluoro-1-hydroxyethyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl 4-methylbenzyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl allyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl benzoylphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl cyclopropyl methylphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl ethylthiomethyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl trans-cinnamyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
diethyl vinylphosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
dimethyl 2-oxoheptyl phosphonate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
dioctyl phenyl phosphonate
IC50 is 0.092 mM
Homo sapiens
3.3.2.10
dodecyl phosphonic acid
IC50 is 0.040 mM
Homo sapiens
3.3.2.10
Estrone 3-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
L-ascorbic acid 2-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
additional information
inhibition mechanism of sulfates, sulfonates, and phosphonates
Homo sapiens
3.3.2.10
N-acetyl-D-galactosamine 4-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
octadecan-9-yl sulfate
IC50 is 0.021 mM
Homo sapiens
3.3.2.10
Sodium dodecyl sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
sodium dodecyl sulfonate
IC50 is 0.05 mM
Homo sapiens
3.3.2.10
taurocholic acid
IC50 is 0.09 mM
Homo sapiens
3.3.2.10
taurolithocholic acid 3-sulfate
IC50 is above 0.1 mM
Homo sapiens
3.3.2.10
tetraisopropyl methylenediphosphonate
IC50 is above 0.1 mM
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.3.76
additional information
-
additional information
inhibition kinetics
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.76
additional information
-
additional information
kinetics
Homo sapiens
3.1.3.76
0.0036
-
2'-[2-benzothiazolyl]-6'-hydroxybenzothiazole phosphate
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
3.1.3.76
0.0036
-
farnesyl diphosphate
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
3.1.3.76
0.0036
-
farnesyl monophosphate
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
3.1.3.76
0.0036
-
geranylgeranyl diphosphate
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
3.1.3.76
0.021
-
threo-9,10-phosphonooxyoctadecanoic acid
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
3.1.3.76
0.21
-
4-methylumbelliferyl phosphate
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
3.1.3.76
1.6
-
4-nitrophenyl phosphate
pH 7.0, 30°C, recombinant enzyme
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.76
Mg2+
dependent on
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.76
(9E)-12-sulfonooxyoctadec-9-enoic acid + H2O
-
661168
Homo sapiens
(9E)-12-hydroxyoctadec-9-enoic acid + sulfate
-
-
-
?
3.1.3.76
(9Z)-12-sulfonooxyoctadec-9-enoic acid + H2O
-
661168
Homo sapiens
(9Z)-12-hydroxyoctadec-9-enoic acid + sulfate
-
-
-
?
3.1.3.76
10-sulfonooxy-octadecanoic acid + H2O
-
661168
Homo sapiens
10-hydroxyoctadecanoic acid + phosphate
-
-
-
?
3.1.3.76
2'-[2-benzothiazolyl]-6'-hydroxybenzothiazole phosphate + H2O
i.e. attophos
661168
Homo sapiens
2'-[2-benzothiazolyl]-6'-hydroxybenzothiazole + phosphate
-
-
-
?
3.1.3.76
4-methylumbelliferyl phosphate + H2O
-
661168
Homo sapiens
4-methylumbelliferone + phosphate
-
-
-
?
3.1.3.76
4-nitrophenyl phosphate + H2O
-
661168
Homo sapiens
4-nitrophenol + phosphate
-
-
-
?
3.1.3.76
farnesyl diphosphate + H2O
-
661168
Homo sapiens
farnesol + phosphate
-
-
-
?
3.1.3.76
farnesyl phosphate + H2O
-
661168
Homo sapiens
farnesol + phosphate
-
-
-
?
3.1.3.76
geranyl diphosphate + H2O
-
661168
Homo sapiens
geraniol + phosphate
-
-
-
?
3.1.3.76
geranylgeranyl diphosphate + H2O
-
661168
Homo sapiens
geranylgeraniol + phosphate
-
-
-
?
3.1.3.76
threo-9,10-phosphonooxyoctadecanoic acid + H2O
-
661168
Homo sapiens
?
-
-
-
?
3.3.2.10
4-nitrophenyl-trans-2,3-epoxy-3-phenylpropyl carbonate + H2O
-
661168
Homo sapiens
?
-
-
-
?
3.3.2.10
racemic trans-1,3-diphenylpropene oxide + H2O
-
661168
Homo sapiens
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.76
More
the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.76
30
-
assay at
Homo sapiens
3.3.2.10
30
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.76
7
-
assay at
Homo sapiens
3.3.2.10
7.4
-
assay at
Homo sapiens