BRENDA - Enzyme Database

Analysis of a novel diacylglycerol kinase from Dictyostelium discoideum: DGKA

Ostroski, M.; Tu-Sekine, B.; Raben, D.M.; Biochemistry 44, 10199-10207 (2005)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.107
phosphatidic acid
highly stimulating
Dictyostelium discoideum
2.7.1.107
phosphatidylinositol 4,5-bisphosphate
highly stimulating
Dictyostelium discoideum
2.7.1.107
phosphatidylserine
strong activation
Dictyostelium discoideum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.107
additional information
-
additional information
kinetics, follow a Michaelis-Menten mechanism in case of medium-chain diacylglycerols, and are strongly dependent on detergent concentration and sort used in the micelles in the assay in case of longer-chain substrates showing sigmoidal kinetics, overview
Dictyostelium discoideum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.107
ATP + 1,2-diacylglycerol
Dictyostelium discoideum
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
?
2.7.1.107
ATP + 1,2-diacylglycerol
Dictyostelium discoideum HL5
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.107
Dictyostelium discoideum
-
-
-
2.7.1.107
Dictyostelium discoideum HL5
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.107
native enzyme, by ultracentrifugation, gel filtration, and antibody affinity chromatography
Dictyostelium discoideum
Reaction
EC Number
Reaction
Commentary
Organism
2.7.1.107
ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
catalytic and regulatory mechanisms
Dictyostelium discoideum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.107
ATP + 1,2-diacylglycerol
-
661157
Dictyostelium discoideum
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-diacylglycerol
-
661157
Dictyostelium discoideum HL5
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-dihexanoyl-sn-glycerol
-
661157
Dictyostelium discoideum
ADP + 1,2-dihexanoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-dihexanoyl-sn-glycerol
-
661157
Dictyostelium discoideum HL5
ADP + 1,2-dihexanoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-dioctanoyl-sn-glycerol
preferred substrate
661157
Dictyostelium discoideum
ADP + 1,2-dioctanoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-dioctanoyl-sn-glycerol
preferred substrate
661157
Dictyostelium discoideum HL5
ADP + 1,2-dioctanoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-dioleoyl-sn-glycerol
-
661157
Dictyostelium discoideum
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-dioleoyl-sn-glycerol
-
661157
Dictyostelium discoideum HL5
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.107
37
-
assay at
Dictyostelium discoideum
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.107
additional information
-
additional information
-
Dictyostelium discoideum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.107
7
-
assay at
Dictyostelium discoideum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.107
ATP
-
Dictyostelium discoideum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.107
phosphatidic acid
highly stimulating
Dictyostelium discoideum
2.7.1.107
phosphatidylinositol 4,5-bisphosphate
highly stimulating
Dictyostelium discoideum
2.7.1.107
phosphatidylserine
strong activation
Dictyostelium discoideum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.107
ATP
-
Dictyostelium discoideum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.107
additional information
-
additional information
kinetics, follow a Michaelis-Menten mechanism in case of medium-chain diacylglycerols, and are strongly dependent on detergent concentration and sort used in the micelles in the assay in case of longer-chain substrates showing sigmoidal kinetics, overview
Dictyostelium discoideum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.107
ATP + 1,2-diacylglycerol
Dictyostelium discoideum
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
?
2.7.1.107
ATP + 1,2-diacylglycerol
Dictyostelium discoideum HL5
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.107
native enzyme, by ultracentrifugation, gel filtration, and antibody affinity chromatography
Dictyostelium discoideum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.107
ATP + 1,2-diacylglycerol
-
661157
Dictyostelium discoideum
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-diacylglycerol
-
661157
Dictyostelium discoideum HL5
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-dihexanoyl-sn-glycerol
-
661157
Dictyostelium discoideum
ADP + 1,2-dihexanoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-dihexanoyl-sn-glycerol
-
661157
Dictyostelium discoideum HL5
ADP + 1,2-dihexanoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-dioctanoyl-sn-glycerol
preferred substrate
661157
Dictyostelium discoideum
ADP + 1,2-dioctanoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-dioctanoyl-sn-glycerol
preferred substrate
661157
Dictyostelium discoideum HL5
ADP + 1,2-dioctanoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-dioleoyl-sn-glycerol
-
661157
Dictyostelium discoideum
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1,2-dioleoyl-sn-glycerol
-
661157
Dictyostelium discoideum HL5
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.107
37
-
assay at
Dictyostelium discoideum
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.107
additional information
-
additional information
-
Dictyostelium discoideum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.107
7
-
assay at
Dictyostelium discoideum