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Literature summary extracted from

  • Vlasie, M.D.; Banerjee, R.
    When a spectator turns killer: suicidal electron transfer from cobalamin in methylmalonyl-CoA mutase (2004), Biochemistry, 43, 8410-8417.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.2 expression in Escherichia coli Propionibacterium freudenreichii subsp. shermanii

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.2 R207Q active site residue, mutation lowering kcat 10000-fold and increasing KM for methylmalonyl-CoA over 30fold Propionibacterium freudenreichii subsp. shermanii
5.4.99.2 R207Q/Y86F double mutant designed to mimic active site of isobutyryl-CoA mutase Propionibacterium freudenreichii subsp. shermanii
5.4.99.2 Y89F active site residue, mutation lowering kcat and increasing KM for methylmalonyl-CoA Propionibacterium freudenreichii subsp. shermanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.2 isobutyryl-CoA R207Q and Y89F/R207Q mutants show conversion of adenosylcobalamin to hydroxycobalamin, indicating inactivation of the enzyme Propionibacterium freudenreichii subsp. shermanii
5.4.99.2 n-butyryl-CoA R207Q and Y89F/R207Q mutants show conversion of adenosylcobalamin to hydroxycobalamin, indicating inactivation of the enzyme. No changes in wild-type and Y89F-mutant Propionibacterium freudenreichii subsp. shermanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.2 additional information
-
additional information KM not detectable, methylmalonyl-CoA, mutant Y89F/R207Q, 37°C Propionibacterium freudenreichii subsp. shermanii
5.4.99.2 additional information
-
additional information KM over 4, methylmalonyl-CoA, mutant R207Q, 37°C Propionibacterium freudenreichii subsp. shermanii
5.4.99.2 0.133
-
methylmalonyl-CoA wild type, 37°C Propionibacterium freudenreichii subsp. shermanii
5.4.99.2 0.357
-
methylmalonyl-CoA mutant Y89F, 37°C Propionibacterium freudenreichii subsp. shermanii

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.2 Propionibacterium freudenreichii subsp. shermanii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.2
-
Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.2 methylmalonyl-CoA
-
Propionibacterium freudenreichii subsp. shermanii succinyl-CoA
-
r

Synonyms

EC Number Synonyms Comment Organism
5.4.99.2 MCM
-
Propionibacterium freudenreichii subsp. shermanii