EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.23 | G190A | no enzymic activity | Mycobacterium tuberculosis |
2.7.1.23 | G198A | no enzymic activity | Mycobacterium tuberculosis |
2.7.1.23 | G207A | no enzymic activity | Mycobacterium tuberculosis |
2.7.1.23 | G208A | decrease in Vmax-value | Mycobacterium tuberculosis |
2.7.1.23 | L192A | no enzymic activity | Mycobacterium tuberculosis |
2.7.1.23 | P196A | no enzymic activity | Mycobacterium tuberculosis |
2.7.1.23 | S199A | decrease in Vmax-value | Mycobacterium tuberculosis |
2.7.1.23 | T195A | kinetic parameters similar to wild-type | Mycobacterium tuberculosis |
2.7.1.23 | T197A | no enzymic activity | Mycobacterium tuberculosis |
2.7.1.23 | T200A | no enzymic activity | Mycobacterium tuberculosis |
2.7.1.23 | Y202A | no enzymic activity | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.23 | NADH | 0.4 mM, 43% residual activity | Mycobacterium tuberculosis | |
2.7.1.23 | NADP+ | 0.4 mM, 22% residual activity | Mycobacterium tuberculosis | |
2.7.1.23 | NADPH | 0.4 mM, 50% residual activity | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.23 | additional information | - |
additional information | allosteric enzyme, Hill coefficient for ATP 1.5, for polyphosphate 1.4 | Mycobacterium tuberculosis | |
2.7.1.23 | 1.2 | - |
NAD+ | cosubstrate polyphosphate, wild-type, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 1.4 | - |
NAD+ | cosubstrate polyphosphate, mutant T195A, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 2.1 | - |
NAD+ | cosubstrate polyphosphate, mutant S199A, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 2.2 | - |
ATP | mutant S199A, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 2.2 | - |
Polyphosphate | mutant S199A, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 2.2 | - |
ATP | mutant T195A, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 2.2 | - |
Polyphosphate | mutant T195A, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 2.5 | - |
ATP | wild-type, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 2.5 | - |
Polyphosphate | wild-type, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 2.7 | - |
NAD+ | cosubstrate ATP, mutant T195A, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 2.7 | - |
ATP | mutant G208A, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 2.7 | - |
Polyphosphate | mutant G208A, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 3.3 | - |
NAD+ | cosubstrate ATP, mutant S199A, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 3.3 | - |
NAD+ | cosubstrate ATP, wild-type, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 3.8 | - |
NAD+ | cosubstrate polyphosphate, mutant G208A, 37°C, pH 8.0 | Mycobacterium tuberculosis | |
2.7.1.23 | 6.5 | - |
NAD+ | cosubstrate ATP, mutant G208A, 37°C, pH 8.0 | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.23 | 33000 | - |
or dimer, 3 * 33000, SDS-PAGE | Mycobacterium tuberculosis |
2.7.1.23 | 33000 | - |
or trimer, 2 * 33000, SDS-PAGE | Mycobacterium tuberculosis |
2.7.1.23 | 82000 | - |
gel filtration | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.23 | Mycobacterium tuberculosis | - |
expression in Escherichia coli | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.23 | recombinant enzyme | Mycobacterium tuberculosis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.23 | 0.5 | - |
substrates ATP, NAD+, mutant G208A, 37°C, pH 8.0 | Mycobacterium tuberculosis |
2.7.1.23 | 0.7 | - |
substrates ATP, NAD+, mutant S199A, 37°C, pH 8.0 | Mycobacterium tuberculosis |
2.7.1.23 | 1.1 | - |
substrates ATP, NAD+, mutant T195A, 37°C, pH 8.0 | Mycobacterium tuberculosis |
2.7.1.23 | 1.2 | - |
substrates ATP, NAD+, wild-type, 37°C, pH 8.0 | Mycobacterium tuberculosis |
2.7.1.23 | 2.2 | - |
substrates polyphosphate, NAD+, mutant G208A, 37°C, pH 8.0 | Mycobacterium tuberculosis |
2.7.1.23 | 2.5 | - |
substrates polyphosphate, NAD+, mutant S199A, 37°C, pH 8.0 | Mycobacterium tuberculosis |
2.7.1.23 | 3.5 | - |
substrates polyphosphate, NAD+, mutant T195A, 37°C, pH 8.0 | Mycobacterium tuberculosis |
2.7.1.23 | 3.8 | - |
substrates polyphosphate, NAD+, wild-type, 37°C, pH 8.0 | Mycobacterium tuberculosis |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.23 | -20°C, stable for several weeks | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.23 | ATP + NAD+ | - |
Mycobacterium tuberculosis | ADP + NADP+ | - |
? | |
2.7.1.23 | polyphosphate + NAD+ | - |
Mycobacterium tuberculosis | (phosphate)n-1 + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.23 | dimer | or trimer, 2 * 33000, SDS-PAGE | Mycobacterium tuberculosis |
2.7.1.23 | trimer | or dimer, 3 * 33000, SDS-PAGE | Mycobacterium tuberculosis |