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Literature summary extracted from

  • Raffaelli, N.; Finaurini, L.; Mazzola, F.; Pucci, L.; Sorci, L.; Amici, A.; Magni, G.
    Characterization of Mycobacterium tuberculosis NAD kinase: functional analysis of the full-length enzyme by site-directed mutagenesis (2004), Biochemistry, 43, 7610-7617.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.23 G190A no enzymic activity Mycobacterium tuberculosis
2.7.1.23 G198A no enzymic activity Mycobacterium tuberculosis
2.7.1.23 G207A no enzymic activity Mycobacterium tuberculosis
2.7.1.23 G208A decrease in Vmax-value Mycobacterium tuberculosis
2.7.1.23 L192A no enzymic activity Mycobacterium tuberculosis
2.7.1.23 P196A no enzymic activity Mycobacterium tuberculosis
2.7.1.23 S199A decrease in Vmax-value Mycobacterium tuberculosis
2.7.1.23 T195A kinetic parameters similar to wild-type Mycobacterium tuberculosis
2.7.1.23 T197A no enzymic activity Mycobacterium tuberculosis
2.7.1.23 T200A no enzymic activity Mycobacterium tuberculosis
2.7.1.23 Y202A no enzymic activity Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.23 NADH 0.4 mM, 43% residual activity Mycobacterium tuberculosis
2.7.1.23 NADP+ 0.4 mM, 22% residual activity Mycobacterium tuberculosis
2.7.1.23 NADPH 0.4 mM, 50% residual activity Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.23 additional information
-
additional information allosteric enzyme, Hill coefficient for ATP 1.5, for polyphosphate 1.4 Mycobacterium tuberculosis
2.7.1.23 1.2
-
NAD+ cosubstrate polyphosphate, wild-type, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 1.4
-
NAD+ cosubstrate polyphosphate, mutant T195A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 2.1
-
NAD+ cosubstrate polyphosphate, mutant S199A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 2.2
-
ATP mutant S199A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 2.2
-
Polyphosphate mutant S199A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 2.2
-
ATP mutant T195A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 2.2
-
Polyphosphate mutant T195A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 2.5
-
ATP wild-type, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 2.5
-
Polyphosphate wild-type, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 2.7
-
NAD+ cosubstrate ATP, mutant T195A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 2.7
-
ATP mutant G208A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 2.7
-
Polyphosphate mutant G208A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 3.3
-
NAD+ cosubstrate ATP, mutant S199A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 3.3
-
NAD+ cosubstrate ATP, wild-type, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 3.8
-
NAD+ cosubstrate polyphosphate, mutant G208A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 6.5
-
NAD+ cosubstrate ATP, mutant G208A, 37°C, pH 8.0 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.23 33000
-
or dimer, 3 * 33000, SDS-PAGE Mycobacterium tuberculosis
2.7.1.23 33000
-
or trimer, 2 * 33000, SDS-PAGE Mycobacterium tuberculosis
2.7.1.23 82000
-
gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Mycobacterium tuberculosis
-
expression in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.23 recombinant enzyme Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.23 0.5
-
substrates ATP, NAD+, mutant G208A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 0.7
-
substrates ATP, NAD+, mutant S199A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 1.1
-
substrates ATP, NAD+, mutant T195A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 1.2
-
substrates ATP, NAD+, wild-type, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 2.2
-
substrates polyphosphate, NAD+, mutant G208A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 2.5
-
substrates polyphosphate, NAD+, mutant S199A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 3.5
-
substrates polyphosphate, NAD+, mutant T195A, 37°C, pH 8.0 Mycobacterium tuberculosis
2.7.1.23 3.8
-
substrates polyphosphate, NAD+, wild-type, 37°C, pH 8.0 Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
2.7.1.23 -20°C, stable for several weeks Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ATP + NAD+
-
Mycobacterium tuberculosis ADP + NADP+
-
?
2.7.1.23 polyphosphate + NAD+
-
Mycobacterium tuberculosis (phosphate)n-1 + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.23 dimer or trimer, 2 * 33000, SDS-PAGE Mycobacterium tuberculosis
2.7.1.23 trimer or dimer, 3 * 33000, SDS-PAGE Mycobacterium tuberculosis