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Literature summary extracted from

  • Lansdon, E.B.; Fisher, A.J.; Segel, I.H.
    Human 3'-phosphoadenosine 5'-phosphosulfate synthetase (isoform 1, brain): kinetic properties of the adenosine triphosphate sulfurylase and adenosine 5'-phosphosulfate kinase domains (2004), Biochemistry, 43, 4356-4365.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.25 dithiothreitol
-
 Homo sapiens
2.7.7.4 dithiothreitol activates Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.25 expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Homo sapiens
2.7.7.4
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.25 adenosine 5'-phosphosulfate substrate inhibition Homo sapiens
2.7.7.4 adenosine 5'-phosphosulfate competitive with ATP and molybdate Homo sapiens
2.7.7.4 ATP
-
 Homo sapiens
2.7.7.4 molybdate
-
 Homo sapiens
2.7.7.4 sulfate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.25 0.0042
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C Homo sapiens
2.7.1.25 0.14
-
 ATP pH 8.0, 30°C Homo sapiens
2.7.1.25 0.14
-
 ATP ATP in form of MgATP2- Homo sapiens
2.7.7.4 0.0048
-
 adenylylsulfate pH 8.0, 30°C Homo sapiens
2.7.7.4 0.0346
-
 diphosphate pH 8.0, 30°C Homo sapiens
2.7.7.4 0.1
-
 ATP pH 8.0, 30°C, reaction with molybdate Homo sapiens
2.7.7.4 0.15
-
 ATP pH 8.0, 30°C, reaction with sulfate Homo sapiens
2.7.7.4 0.16
-
 sulfate pH 8.0, 30°C Homo sapiens
2.7.7.4 1.3
-
 molybdate pH 8.0, 30°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.25 Mg2+ in complex with ATP Homo sapiens
2.7.1.25 NaCl increased activity at high salt conditions, 100 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 71000
-
 2 * 71000 Homo sapiens
2.7.1.25 150000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.25 ATP + adenosine 5'-phosphosulfate Homo sapiens synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Homo sapiens O43252 isoform 1
-
2.7.7.4 Homo sapiens O43252 recombinant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25 recombinant enzyme using His-tag Homo sapiens
2.7.7.4
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.25 brain
-
 Homo sapiens
-
2.7.7.4 brain
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.25 0.12
-
 pH 8.0. 30°C Homo sapiens
2.7.7.4 0.00012
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5'-phosphosulfate
-
 Homo sapiens ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?
2.7.1.25 ATP + adenosine 5'-phosphosulfate synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate Homo sapiens ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?
2.7.1.25 ATP + adenylyl sulfate
-
 Homo sapiens ADP + 3'-phosphoadenylyl sulfate
-
 r
2.7.7.4 ATP + MoO42-
-
 Homo sapiens AMP + adenylylmolybdate
-
 ?
2.7.7.4 ATP + sulfate
-
 Homo sapiens diphosphate + adenylylsulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.25 dimer 2 * 71000 Homo sapiens
2.7.7.4 More native enzyme, a dimer with each 71 kDa subunit containing an adenosine triphosphate sulfurylase and an adenosine 5'-hosphosulfate kinase domain, catalyzes the overall formation of PAPS from ATP and inorganic sulfate Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.25 More bifunctional enzyme with ATP sulfurylase activity and adenosine phosphosulfate kinase activity, catalyzes the formation of 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate Homo sapiens
2.7.7.4 adenosine triphosphate sulfurylase native enzyme, a dimer with each 71 kDa subunit containing an adenosine triphosphate sulfurylase and an adenosine 5'-hosphosulfate kinase domain, catalyzes the overall formation of PAPS from ATP and inorganic sulfate Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.25 0.048
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C Homo sapiens
2.7.7.4 0.000018
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C Homo sapiens
2.7.7.4 1
-
 ATP pH 8.0, 30°C, reaction with molybdate Homo sapiens
2.7.7.4 1.1
-
 ATP pH 8.0, 30°C, reaction with sulfate Homo sapiens
2.7.7.4 1.7
-
 sulfate pH 8.0, 30°C Homo sapiens
2.7.7.4 14.3
-
 molybdate pH 8.0, 30°C Homo sapiens