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Literature summary extracted from

  • Epand, R.M.; Kam, A.; Bridgelal, N.; Saiga, A.; Topham, M.K.
    The alpha isoform of diacylglycerol kinase exhibits arachidonoyl specificity with alkylacylglycerol (2004), Biochemistry, 43, 14778-14783.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 additional information addition of cholesterol and/or phosphatidylethanolamine reduce the substrate specificity Mammalia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 additional information
-
 additional information Km values of isozymes DGKzeta, DGKepsilon, and DGKalpha with different substrates in mol% Mammalia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 membrane
-
 Mammalia 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Ca2+ dependent on, maximal below 0.01 mM Mammalia
2.7.1.107 Mg2+
-
 Mammalia
2.7.1.107 additional information effects of liposome composition on substrate specificity and catalytic activity level of the isozymes Mammalia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol Mammalia
-
 ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Mammalia
-
 9 different isozymes
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.107 additional information tissue-specific expression of isozymes Mammalia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.107 additional information
-
 activity measurment of enzyme solubilized in liposomes consisting different amounts of 1,2-dioleoylphosphatidylcholine, 1,2-dioleoylphosphatidylethanolamine, and 1,2-dioleoylphosphatidylserine, overview Mammalia
2.7.1.107 0.0002 0.018 substrate specificity and catalytic activity level of the isozymes at different liposome compositions Mammalia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol
-
 Mammalia ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1,2-dioleoyl-sn-glycerol
-
 Mammalia ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1-O-hexanoyl-2-arachidonoyl-sn-glycerol preferred by isozyme DGKzeta and isozyme DGKalpha Mammalia ADP + 1-O-hexanoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1-O-hexanoyl-2-oleoyl-sn-glycerol
-
 Mammalia ADP + 1-O-hexanoyl-2-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
-
 Mammalia ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 additional information isozymes DGKzeta, DGKalpha, and DGKepsilon utilize 1-alkyl-2-acyl-glycerols as substrates, addition of cholesterol and/or phosphatidylethanolamine reduce the substrate specificity Mammalia ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.107 DGK
-
 Mammalia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.107 25
-
 assay at Mammalia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.107 7.5 8 assay at Mammalia

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.107 ATP
-
 Mammalia