EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.6 | wild-type and mutant enzymes, expression in Escherichia coli BL21(DE3) | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.6 | E240D | 1.2fold increase in Km-value for Lys, 21fold decrease in turnover number for Lys, Km-value for ATP is nearly identical to wild-type value, 24fold decrease in turnover number for ATP | Escherichia coli |
6.1.1.6 | E240Q | 1.4fold increase in Km-value for Lys, 207fold decrease in turnover number for Lys, 1.2fold increase in Km-value for ATP, 261fold decrease in turnover number for ATP | Escherichia coli |
6.1.1.6 | E278D | 98fold increase in Km-value for Lys, 11fold decrease in turnover number for Lys, 1.3fold increase in Km-value for ATP, 63fold decrease in turnover number for ATP | Escherichia coli |
6.1.1.6 | E278Q | 1.9fold decrease in Km-value for Lys, 120fold decrease in turnover number for Lys, 1.5fold decrease in Km-value for ATP, 200fold decrease in turnover number for ATP | Escherichia coli |
6.1.1.6 | E428D | 8.5fold increase in Km-value for Lys, 1.8fold decrease in turnover number for Lys, 1.7fold increase in Km-value for ATP, 3.4fold decrease in turnover number for ATP | Escherichia coli |
6.1.1.6 | E428Q | 2fold decrease in Km-value for Lys, 9fold decrease in turnover number for Lys, 1.5fold decrease in Km-value for ATP, 200fold decrease in turnover number for ATP | Escherichia coli |
6.1.1.6 | F426H | 78fold increase in Km-value for Lys, 1.3fold decrease in turnover number for Lys, 1.6fold increase in Km-value for ATP, 5fold decrease in turnover number for ATP | Escherichia coli |
6.1.1.6 | F426W | 6.2fold increase in Km-value for Lys, 3fold decrease in turnover number for Lys, 9.2fold increase in Km-value for ATP, 1.4fold decrease in turnover number for ATP | Escherichia coli |
6.1.1.6 | G216A | 75fold increase in Km-value for Lys, 1.9fold increase in turnover number for Lys, 16fold increase in Km-value for ATP, 4.25fold decrease in turnover number for ATP | Escherichia coli |
6.1.1.6 | N424D | 2fold increase in Km-value for Lys, 1.1fold decrease in turnover number for Lys, 1.8fold increase in Km-value for ATP, 3.4fold decrease in turnover number for ATP | Escherichia coli |
6.1.1.6 | N424Q | 20fold increase in Km-value for Lys, 2.8fold decrease in turnover number for Lys, 69fold increase in Km-value for ATP, 1.5fold decrease in turnover number for ATP | Escherichia coli |
6.1.1.6 | Y280F | 9.2fold increase in Km-value for Lys, 58fold decrease in turnover number for Lys, 5.2fold increase in Km-value for ATP, 20fold decrease in turnover number for ATP | Escherichia coli |
6.1.1.6 | Y280S | 44fold increase in Km-value for Lys, 1.1fold decrease in turnover number for Lys, 3.9fold increase in Km-value for ATP, 9.7fold decrease in turnover number for ATP | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.6 | 5'-O-[N-(L-lysyl)sulfamoyl]adenosine | - |
Escherichia coli | |
6.1.1.6 | S-(2-aminoethyl)-L-cysteine | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.6 | 0.0013 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E428Q | Escherichia coli | |
6.1.1.6 | 0.0014 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E278Q | Escherichia coli | |
6.1.1.6 | 0.0026 | - |
L-lysine | pH 7.2, 37°C, wild-type enzyme | Escherichia coli | |
6.1.1.6 | 0.0033 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E240D | Escherichia coli | |
6.1.1.6 | 0.0036 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E240Q | Escherichia coli | |
6.1.1.6 | 0.0052 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme N424D | Escherichia coli | |
6.1.1.6 | 0.0078 | - |
ATP | pH 7.2, 37°C, mutant enzyme E278Q | Escherichia coli | |
6.1.1.6 | 0.0078 | - |
ATP | pH 7.2, 37°C, mutant enzyme E428Q | Escherichia coli | |
6.1.1.6 | 0.011 | - |
ATP | pH 7.2, 37°C, mutant enzyme E240D | Escherichia coli | |
6.1.1.6 | 0.012 | - |
ATP | pH 7.2, 37°C, wild-type enzyme | Escherichia coli | |
6.1.1.6 | 0.014 | - |
ATP | pH 7.2, 37°C, mutant enzyme E240Q | Escherichia coli | |
6.1.1.6 | 0.016 | - |
ATP | pH 7.2, 37°C, mutant enzyme E278D | Escherichia coli | |
6.1.1.6 | 0.016 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme F426W | Escherichia coli | |
6.1.1.6 | 0.019 | - |
ATP | pH 7.2, 37°C, mutant enzyme F426H | Escherichia coli | |
6.1.1.6 | 0.02 | - |
ATP | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
6.1.1.6 | 0.021 | - |
ATP | pH 7.2, 37°C, mutant enzyme N424D | Escherichia coli | |
6.1.1.6 | 0.022 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
6.1.1.6 | 0.024 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme Y280F | Escherichia coli | |
6.1.1.6 | 0.052 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme N424Q | Escherichia coli | |
6.1.1.6 | 0.062 | - |
ATP | pH 7.2, 37°C, mutant enzyme Y280F | Escherichia coli | |
6.1.1.6 | 0.11 | - |
ATP | pH 7.2, 37°C, mutant enzyme F426W | Escherichia coli | |
6.1.1.6 | 0.114 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme Y280S | Escherichia coli | |
6.1.1.6 | 0.147 | - |
ATP | pH 7.2, 37°C, mutant enzyme Y280S | Escherichia coli | |
6.1.1.6 | 0.195 | - |
ATP | pH 7.2, 37°C, mutant enzyme G216A | Escherichia coli | |
6.1.1.6 | 0.196 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme G216A | Escherichia coli | |
6.1.1.6 | 0.203 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme F426H | Escherichia coli | |
6.1.1.6 | 0.254 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E278D | Escherichia coli | |
6.1.1.6 | 0.83 | - |
ATP | pH 7.2, 37°C, mutant enzyme N424Q | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.6 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.6 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.6 | ATP + L-lysine + tRNALys | - |
Escherichia coli | AMP + L-lysyl-tRNALys + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.6 | class II lysyl-tRNA synthetase | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.6 | 0.0087 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E240Q | Escherichia coli | |
6.1.1.6 | 0.013 | - |
ATP | pH 7.2, 37°C, mutant enzyme E240Q | Escherichia coli | |
6.1.1.6 | 0.015 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E278Q | Escherichia coli | |
6.1.1.6 | 0.017 | - |
ATP | pH 7.2, 37°C, mutant enzyme E278Q | Escherichia coli | |
6.1.1.6 | 0.017 | - |
ATP | pH 7.2, 37°C, mutant enzyme E428Q | Escherichia coli | |
6.1.1.6 | 0.02 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
6.1.1.6 | 0.031 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme Y280F | Escherichia coli | |
6.1.1.6 | 0.054 | - |
ATP | pH 7.2, 37°C, mutant enzyme E278D | Escherichia coli | |
6.1.1.6 | 0.086 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E240D | Escherichia coli | |
6.1.1.6 | 0.14 | - |
ATP | pH 7.2, 37°C, mutant enzyme E240D | Escherichia coli | |
6.1.1.6 | 0.16 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E278D | Escherichia coli | |
6.1.1.6 | 0.17 | - |
ATP | pH 7.2, 37°C, mutant enzyme Y280F | Escherichia coli | |
6.1.1.6 | 0.35 | - |
ATP | pH 7.2, 37°C, mutant enzyme Y280S | Escherichia coli | |
6.1.1.6 | 0.6 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme F426W | Escherichia coli | |
6.1.1.6 | 0.65 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme N424Q | Escherichia coli | |
6.1.1.6 | 0.67 | - |
ATP | pH 7.2, 37°C, mutant enzyme F426H | Escherichia coli | |
6.1.1.6 | 0.8 | - |
ATP | pH 7.2, 37°C, mutant enzyme G216A | Escherichia coli | |
6.1.1.6 | 1 | - |
ATP | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
6.1.1.6 | 1 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
6.1.1.6 | 1 | - |
ATP | pH 7.2, 37°C, mutant enzyme N424D | Escherichia coli | |
6.1.1.6 | 1.6 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme N424D | Escherichia coli | |
6.1.1.6 | 1.6 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme Y280S | Escherichia coli | |
6.1.1.6 | 1.8 | - |
L-lysine | pH 7.2, 37°C, wild-type enzyme | Escherichia coli | |
6.1.1.6 | 2.2 | - |
ATP | pH 7.2, 37°C, mutant enzyme N424Q | Escherichia coli | |
6.1.1.6 | 2.4 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme F426H | Escherichia coli | |
6.1.1.6 | 2.4 | - |
ATP | pH 7.2, 37°C, mutant enzyme F426W | Escherichia coli | |
6.1.1.6 | 3.4 | - |
ATP | pH 7.2, 37°C, wild-type enzyme | Escherichia coli | |
6.1.1.6 | 3.4 | - |
L-lysine | pH 7.2, 37°C, mutant enzyme G216A | Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.6 | 0.00001 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme E240Q | Escherichia coli | |
6.1.1.6 | 0.000011 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme E428Q | Escherichia coli | |
6.1.1.6 | 0.000023 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme E278Q | Escherichia coli | |
6.1.1.6 | 0.000028 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, wild-type enzyme | Escherichia coli | |
6.1.1.6 | 0.000029 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme E240D | Escherichia coli | |
6.1.1.6 | 0.000039 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme N424D | Escherichia coli | |
6.1.1.6 | 0.000041 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme E278D | Escherichia coli | |
6.1.1.6 | 0.00005 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme F426W | Escherichia coli | |
6.1.1.6 | 0.000055 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme N424Q | Escherichia coli | |
6.1.1.6 | 0.000059 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme G216A | Escherichia coli | |
6.1.1.6 | 0.000061 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
6.1.1.6 | 0.00022 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme Y280S | Escherichia coli | |
6.1.1.6 | 0.00027 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme Y280F | Escherichia coli | |
6.1.1.6 | 0.0013 | - |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | pH 7.2, 37°C, mutant enzyme F426H | Escherichia coli | |
6.1.1.6 | 0.0015 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme E428Q | Escherichia coli | |
6.1.1.6 | 0.0031 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme E240D | Escherichia coli | |
6.1.1.6 | 0.0039 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, wild-type enzyme | Escherichia coli | |
6.1.1.6 | 0.004 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme N424D | Escherichia coli | |
6.1.1.6 | 0.005 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme E278Q | Escherichia coli | |
6.1.1.6 | 0.0054 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme E240Q | Escherichia coli | |
6.1.1.6 | 0.025 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme F426W | Escherichia coli | |
6.1.1.6 | 0.034 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme E428D | Escherichia coli | |
6.1.1.6 | 0.072 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme Y280F | Escherichia coli | |
6.1.1.6 | 0.076 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme N424Q | Escherichia coli | |
6.1.1.6 | 0.34 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme Y280S | Escherichia coli | |
6.1.1.6 | 1.24 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme G216A | Escherichia coli | |
6.1.1.6 | 1.29 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme E278D | Escherichia coli | |
6.1.1.6 | 1.31 | - |
S-(2-aminoethyl)-L-cysteine | pH 7.2, 37°C, mutant enzyme F426H | Escherichia coli |