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Literature summary extracted from

  • Anand, R.; Hoskins, A.A.; Stubbe, J.; Ealick, S.E.
    Domain organization of Salmonella typhimurium formylglycinamide ribonucleotide amidotransferase revealed by X-ray crystallography (2004), Biochemistry, 43, 10328-10342.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.3 expression in Escherichia coli B834(DE3) Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.5.3 crystals of the pure SeMet-enzyme are grown at room temperature using hanging-drop vapor diffusion method. StPurL crystallizes in the hexagonal space group P6(5) with unit cell dimensions of a = 145.3 and c = 140.9 A. The asymmetric unit contains one monomer, corresponding to calculated solvent content of 57%. Domain organization revealed by X-ray crystallography Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.3 formylglycinamide ribonucleotide + ATP + L-glutamine + H2O Salmonella enterica subsp. enterica serovar Typhimurium fourth step of purine biosynthetic pathway formylglycinamidine ribonucleotide + ADP + phosphate + L-glutamate
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Organism

EC Number Organism UniProt Comment Textmining
6.3.5.3 Salmonella enterica subsp. enterica serovar Typhimurium
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.3 formylglycinamide ribonucleotide + ATP + L-glutamine + H2O
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 Salmonella enterica subsp. enterica serovar Typhimurium formylglycinamidine ribonucleotide + ADP + phosphate + L-glutamate
-
 ?
6.3.5.3 formylglycinamide ribonucleotide + ATP + L-glutamine + H2O fourth step of purine biosynthetic pathway Salmonella enterica subsp. enterica serovar Typhimurium formylglycinamidine ribonucleotide + ADP + phosphate + L-glutamate
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 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.3 StPurL
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 Salmonella enterica subsp. enterica serovar Typhimurium