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Literature summary extracted from

  • Kumar, P.; Brushia, R.J.; Hoye, E.; Walsh, D.A.
    Baculovirus-mediated overexpression of the phosphorylase b kinase holoenzyme and alpha gamma delta and gamma delta subcomplexes (2004), Biochemistry, 43, 10247-10254.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.19 cooverexpression with the beta subunit of the rabbit enzyme with the rat holoenzyme and rat alphagammadelta and gammadelta subunit complexes in Spodoptera frugiperda Sf9 cells via the baculovirus infection system, resulting in formation of subunit subcomplexes, overview Oryctolagus cuniculus
2.7.11.19 subcloning of subunits in Escherichia coli, overexpression of the soluble holoenzyme and of soluble trimeric alphagammadelta and dimeric gammadelta subunit subcomplexes, as well as coexpression with the beta subunit of the rabbit enzyme also resulting in subunit complex formation, in Spodoptera frugiperda Sf9 cells via the baculovirus infection system Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Ca2+ activates, dependent on, required for binding of calmodulin Oryctolagus cuniculus
2.7.11.19 Ca2+ activates, dependent on, required for binding of calmodulin Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Oryctolagus cuniculus
-
-
-
2.7.11.19 Rattus norvegicus Q64649 alpha subunit of the skeletal muscle enzyme
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.19 additional information the native and the recombinant enzyme performs autophosphorylation of its alpha and beta subunits Oryctolagus cuniculus
2.7.11.19 phosphoprotein regulation by de-/phosphorylation performed by cAMP-dependent protein kinase, EC 2.7.11.11 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19 native enzyme from skeletal muscle, recombinant enzyme subunit subcomplexes from Sf9 insect cells Oryctolagus cuniculus
2.7.11.19 recombinant holoenzyme and enzyme subunit subcomplexes from Sf9 insect cells Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 skeletal muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.19 additional information
-
 activity of purified native and recombinant enzymes and subunit subcomplexes at different pH Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 additional information the enzyme performs autophosphorylation Oryctolagus cuniculus ?
-
 ?
2.7.11.19 additional information the enzyme performs autophosphorylation Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.19 hexadecamer (alphabetagammadelta)4 Rattus norvegicus
2.7.11.19 More the delta subunit is identical with calmodulin Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.19 PhK
-
 Oryctolagus cuniculus
2.7.11.19 PhK
-
 Rattus norvegicus
2.7.11.19 Phosphorylase b kinase
-
 Oryctolagus cuniculus
2.7.11.19 Phosphorylase b kinase
-
 Rattus norvegicus
2.7.11.19 SkM Phk
-
 Oryctolagus cuniculus
2.7.11.19 SkM Phk
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.19 30
-
 autophosphorylation reaction at Oryctolagus cuniculus
2.7.11.19 30
-
 autophosphorylation reaction at Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.19 additional information
-
 activity of purified native and recombinant enzymes and subunit subcomplexes at different pH, the latter show higher activity at pH 6.8, while the native enzyme shows higher activity at pH 8.2 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.19 ATP
-
 Oryctolagus cuniculus
2.7.11.19 ATP
-
 Rattus norvegicus
2.7.11.19 Calmodulin Ca2+-dependent binding, identical with the delta subunit Oryctolagus cuniculus
2.7.11.19 Calmodulin Ca2+-dependent binding, identical with the delta subunit Rattus norvegicus