EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.1.145 | purified recombinant enzyme in complex with dT and dTPP, hanging drop vapour diffusion method, enzyme solution containing 5-10 mg/ml protein, 5 mM dT or dTTP, is mixed in equal volumes with cyrstallization solution containing 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 18-22% w/v PEG 5000 monomethylether, and 5-10% w/v PEG 400, 3-5 days, 14°C, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.145 | dTTP | competitive versus ATP inpresence of 2'-deoxythymidine as substrate, feedback inhibition, mechanism, binding structure at the active site, overview | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.145 | ATP + 2'-deoxyadenosine | Drosophila melanogaster | - |
ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxycytidine | Drosophila melanogaster | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyguanosine | Drosophila melanogaster | - |
ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxythymidine | Drosophila melanogaster | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.145 | Drosophila melanogaster | Q9XZT6 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.145 | ATP + 2'-deoxyadenosine | - |
Drosophila melanogaster | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxycytidine | - |
Drosophila melanogaster | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyguanosine | - |
Drosophila melanogaster | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxythymidine | - |
Drosophila melanogaster | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxythymidine | 2'-deoxythymidine binding structure at the active site, overview | Drosophila melanogaster | ADP + 2'-deoxythymidine 5'-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.145 | deoxyribonucleoside kinase | - |
Drosophila melanogaster |
2.7.1.145 | dNK | - |
Drosophila melanogaster |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.145 | additional information | - |
additional information | feedback inhibition kinetics | Drosophila melanogaster |