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Structural basis for feedback inhibition of the deoxyribonucleoside salvage pathway: studies of the Drosophila deoxyribonucleoside kinase

Mikkelsen, N.E.; Johansson, K.; Karlsson, A.; Knecht, W.; Andersen, G.; Piskur, J.; Munch-Petersen, B.; Eklund, H.; Biochemistry 42, 5706-5712 (2003)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
2.7.1.145
purified recombinant enzyme in complex with dT and dTPP, hanging drop vapour diffusion method, enzyme solution containing 5-10 mg/ml protein, 5 mM dT or dTTP, is mixed in equal volumes with cyrstallization solution containing 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 18-22% w/v PEG 5000 monomethylether, and 5-10% w/v PEG 400, 3-5 days, 14°C, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution
Drosophila melanogaster
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.145
dTTP
competitive versus ATP inpresence of 2'-deoxythymidine as substrate, feedback inhibition, mechanism, binding structure at the active site, overview
Drosophila melanogaster
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.145
ATP + 2'-deoxyadenosine
Drosophila melanogaster
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
Drosophila melanogaster
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
Drosophila melanogaster
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
Drosophila melanogaster
-
ADP + 2'-deoxythymidine 5'-phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.145
Drosophila melanogaster
Q9XZT6
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
ATP + 2'-deoxyadenosine
-
661072
Drosophila melanogaster
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
-
661072
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
661072
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
-
661072
Drosophila melanogaster
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
2'-deoxythymidine binding structure at the active site, overview
661072
Drosophila melanogaster
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.145
additional information
-
additional information
feedback inhibition kinetics
Drosophila melanogaster
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.7.1.145
purified recombinant enzyme in complex with dT and dTPP, hanging drop vapour diffusion method, enzyme solution containing 5-10 mg/ml protein, 5 mM dT or dTTP, is mixed in equal volumes with cyrstallization solution containing 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 18-22% w/v PEG 5000 monomethylether, and 5-10% w/v PEG 400, 3-5 days, 14°C, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution
Drosophila melanogaster
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.145
dTTP
competitive versus ATP inpresence of 2'-deoxythymidine as substrate, feedback inhibition, mechanism, binding structure at the active site, overview
Drosophila melanogaster
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.145
additional information
-
additional information
feedback inhibition kinetics
Drosophila melanogaster
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.145
ATP + 2'-deoxyadenosine
Drosophila melanogaster
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
Drosophila melanogaster
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
Drosophila melanogaster
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
Drosophila melanogaster
-
ADP + 2'-deoxythymidine 5'-phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
ATP + 2'-deoxyadenosine
-
661072
Drosophila melanogaster
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
-
661072
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
661072
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
-
661072
Drosophila melanogaster
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxythymidine
2'-deoxythymidine binding structure at the active site, overview
661072
Drosophila melanogaster
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?