Data extracted from this reference:
Cloned(Commentary)
2.7.1.21
DNA and amino acid sequence determination, expression in Escherichia coli as maltose binding fusion protein in inclusion bodies, coexpression of GroEL/ES molecular chaperone system improves enzyme solubility only marginally, but ethanol supplementation contributes to enzyme recovery due to activation of heat shock response
Homo sapiens
KM Value [mM]
2.7.1.21
additional information
additional information
kinetics
Homo sapiens
2.7.1.21
0.0047
ATP
dimeric enzyme, pH 7.5, 25°C
Homo sapiens
2.7.1.21
0.0109
ATP
tetrameric enzyme, pH 7.5, 25°C
Homo sapiens
2.7.1.21
0.018
2'-deoxycytidine
tetrameric enzyme, pH 7.5, 25°C
Homo sapiens
2.7.1.21
0.025
2'-deoxycytidine
dimeric enzyme, pH 7.5, 25°C
Homo sapiens
2.7.1.21
0.05
2'-deoxythymidine
tetrameric enzyme, pH 7.5, 25°C
Homo sapiens
2.7.1.21
0.054
2'-deoxythymidine
dimeric enzyme, pH 7.5, 25°C
Homo sapiens
Localization
2.7.1.21
cytosol
Homo sapiens
5829
2.7.1.21
mitochondrion
Homo sapiens
5739
Metals/Ions
2.7.1.21
Mg2+
Homo sapiens
Molecular Weight [Da]
2.7.1.21
additional information
oligomeric states have MWs of 152200 and 95900, as determined by gel filtration
Homo sapiens
2.7.1.21
48800
recombinant enzyme, gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
2.7.1.21
ATP + 2'-deoxycytidine
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
ir
2.7.1.21
ATP + 2'-deoxythymidine
Homo sapiens
ADP + 2'-deoxythymidine 5'-phosphate
ir
2.7.1.21
ATP + 2'-deoxyuridine
Homo sapiens
ADP + 2'-deoxyuridine 5'-phosphate
ir
Organism
2.7.1.21
Homo sapiens
O00142
Purification (Commentary)
2.7.1.21
recombinant enzyme as maltose binding fusion protein from Escherichia coli
Homo sapiens
Reaction
2.7.1.21
ATP + thymidine = ADP + dTMP
molecular docking of substrates and inhibitors, active site structure modeling
Homo sapiens
Renatured (Commentary)
2.7.1.21
coexpression of GroEL/ES molecular chaperone system improves recombinant enzyme solubility from inclusion bodies only marginally, but ethanol supplementation contributes to enzyme recovery due to activation of heat shock response
Homo sapiens
Source Tissue
2.7.1.21
additional information
expression in nonproliferating tissue
Homo sapiens
Substrates and Products (Substrate)
2.7.1.21
ATP + 2'-deoxycytidine
661066
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
ir
2.7.1.21
ATP + 2'-deoxythymidine
661066
Homo sapiens
ADP + 2'-deoxythymidine 5'-phosphate
ir
2.7.1.21
ATP + 2'-deoxyuridine
661066
Homo sapiens
ADP + 2'-deoxyuridine 5'-phosphate
ir
Subunits
2.7.1.21
dimer
enzyme appears as dimer and tetramer
Homo sapiens
2.7.1.21
More
structure modeling
Homo sapiens
2.7.1.21
tetramer
enzyme appears as dimer and tetramer
Homo sapiens
Synonyms
2.7.1.21
2'-deoxythymidine kinase
Homo sapiens
2.7.1.21
More
the enzyme belongs to the thymidine kinase 2, i.e. TK2, family of enzymes
Homo sapiens
2.7.1.21
thymidine kinase 2
Homo sapiens
2.7.1.21
TK2
Homo sapiens
Temperature Optimum [°C]
Temperature Range [°C]
2.7.1.21
25
37
highest activity within this temperature range at 37°C
Homo sapiens
pH Optimum
2.7.1.21
7.5
assay at
Homo sapiens
Cofactor
2.7.1.21
ATP
Homo sapiens
Cloned(Commentary) (protein specific)
2.7.1.21
DNA and amino acid sequence determination, expression in Escherichia coli as maltose binding fusion protein in inclusion bodies, coexpression of GroEL/ES molecular chaperone system improves enzyme solubility only marginally, but ethanol supplementation contributes to enzyme recovery due to activation of heat shock response
Homo sapiens
Cofactor (protein specific)
2.7.1.21
ATP
Homo sapiens
KM Value [mM] (protein specific)
2.7.1.21
additional information
additional information
kinetics
Homo sapiens
2.7.1.21
0.0047
ATP
dimeric enzyme, pH 7.5, 25°C
Homo sapiens
2.7.1.21
0.0109
ATP
tetrameric enzyme, pH 7.5, 25°C
Homo sapiens
2.7.1.21
0.018
2'-deoxycytidine
tetrameric enzyme, pH 7.5, 25°C
Homo sapiens
2.7.1.21
0.025
2'-deoxycytidine
dimeric enzyme, pH 7.5, 25°C
Homo sapiens
2.7.1.21
0.05
2'-deoxythymidine
tetrameric enzyme, pH 7.5, 25°C
Homo sapiens
2.7.1.21
0.054
2'-deoxythymidine
dimeric enzyme, pH 7.5, 25°C
Homo sapiens
Localization (protein specific)
2.7.1.21
cytosol
Homo sapiens
5829
2.7.1.21
mitochondrion
Homo sapiens
5739
Metals/Ions (protein specific)
2.7.1.21
Mg2+
Homo sapiens
Molecular Weight [Da] (protein specific)
2.7.1.21
additional information
oligomeric states have MWs of 152200 and 95900, as determined by gel filtration
Homo sapiens
2.7.1.21
48800
recombinant enzyme, gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
2.7.1.21
ATP + 2'-deoxycytidine
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
ir
2.7.1.21
ATP + 2'-deoxythymidine
Homo sapiens
ADP + 2'-deoxythymidine 5'-phosphate
ir
2.7.1.21
ATP + 2'-deoxyuridine
Homo sapiens
ADP + 2'-deoxyuridine 5'-phosphate
ir
Purification (Commentary) (protein specific)
2.7.1.21
recombinant enzyme as maltose binding fusion protein from Escherichia coli
Homo sapiens
Renatured (Commentary) (protein specific)
2.7.1.21
coexpression of GroEL/ES molecular chaperone system improves recombinant enzyme solubility from inclusion bodies only marginally, but ethanol supplementation contributes to enzyme recovery due to activation of heat shock response
Homo sapiens
Source Tissue (protein specific)
2.7.1.21
additional information
expression in nonproliferating tissue
Homo sapiens
Substrates and Products (Substrate) (protein specific)
2.7.1.21
ATP + 2'-deoxycytidine
661066
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
ir
2.7.1.21
ATP + 2'-deoxythymidine
661066
Homo sapiens
ADP + 2'-deoxythymidine 5'-phosphate
ir
2.7.1.21
ATP + 2'-deoxyuridine
661066
Homo sapiens
ADP + 2'-deoxyuridine 5'-phosphate
ir
Subunits (protein specific)
2.7.1.21
dimer
enzyme appears as dimer and tetramer
Homo sapiens
2.7.1.21
More
structure modeling
Homo sapiens
2.7.1.21
tetramer
enzyme appears as dimer and tetramer
Homo sapiens
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
2.7.1.21
25
37
highest activity within this temperature range at 37°C
Homo sapiens
pH Optimum (protein specific)
2.7.1.21
7.5
assay at
Homo sapiens