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Literature summary extracted from

  • Clark, E.H.; East, J.M.; Lee, A.G.
    The role of tryptophan residues in an integral membrane protein: diacylglycerol kinase (2003), Biochemistry, 42, 11065-11073.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 expression of His-tagged wild-type and mutant enzymes Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.107 W112L site-directed mutagenesis, inactive mutant Escherichia coli
2.7.1.107 W117L site-directed mutagenesis, inactive mutant Escherichia coli
2.7.1.107 W18L site-directed mutagenesis, reduced activity compared to the wild-type enzyme Escherichia coli
2.7.1.107 W18L/W25L site-directed mutagenesis, reduced activity compared to the wild-type enzyme Escherichia coli
2.7.1.107 W18L/W25L/W112L/W117L site-directed mutagenesis, inactive mutant Escherichia coli
2.7.1.107 W18L/W25L/W47L site-directed mutagenesis, reduced activity compared to the wild-type enzyme Escherichia coli
2.7.1.107 W18L/W25L/W47L/W112L site-directed mutagenesis, inactive mutant Escherichia coli
2.7.1.107 W18L/W25L/W47L/W117L site-directed mutagenesis, reduced activity compared to the wild-type enzyme Escherichia coli
2.7.1.107 W18L/W47L/W112L/W117L site-directed mutagenesis, inactive mutant Escherichia coli
2.7.1.107 W18L/W47L/W117L site-directed mutagenesis, reduced activity compared to the wild-type enzyme Escherichia coli
2.7.1.107 W25L site-directed mutagenesis, reduced activity compared to the wild-type enzyme Escherichia coli
2.7.1.107 W25L/W47L/W112L/W117L site-directed mutagenesis, inactive mutant Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 additional information
-
 additional information kinetics, recombinant wild-type and mutant enzymes Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 membrane integral membrane protein Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol Escherichia coli
-
 ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 recombinant HIs-tagged wild-type and mutant enzymes Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.107 23
-
 purified recombinant mutant W25L Escherichia coli
2.7.1.107 26
-
 purified recombinant mutant W18L/W25L/W47L Escherichia coli
2.7.1.107 36
-
 purified recombinant mutant W18L/W25L/W47L/W117L Escherichia coli
2.7.1.107 42
-
 purified recombinant mutant W18L/W25L Escherichia coli
2.7.1.107 61
-
 purified recombinant mutant W18L Escherichia coli
2.7.1.107 77
-
 purified recombinant mutant W18L/W47L/W117L Escherichia coli
2.7.1.107 102
-
 purified recombinant wild-type enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol
-
 Escherichia coli ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 r
2.7.1.107 ATP + 1,2-diacylglycerol
-
 Escherichia coli ADP + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid r

Synonyms

EC Number Synonyms Comment Organism
2.7.1.107 DGK
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.107 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.107 6.9
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.107 ADP
-
 Escherichia coli
2.7.1.107 ATP
-
 Escherichia coli