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Literature summary extracted from

  • Koppisch, A.T.; Khosla, C.
    Structure-based mutagenesis of the malonyl-CoA:acyl carrier protein transacylase from Streptomyces coelicolor (2003), Biochemistry, 42, 11057-11064.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.39 A197D site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptomyces coelicolor
2.3.1.39 F200A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
2.3.1.39 F200S site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
2.3.1.39 F200Y site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptomyces coelicolor
2.3.1.39 G198A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptomyces coelicolor
2.3.1.39 G198Y site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
2.3.1.39 M126I site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
2.3.1.39 M137L site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
2.3.1.39 Q9A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
2.3.1.39 R122A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
2.3.1.39 R122K site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
2.3.1.39 T57V site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
2.3.1.39 V98Q site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.39 0.015
-
 malonyl-CoA pH 6.8, recombinant mutant M137L Streptomyces coelicolor
2.3.1.39 0.019
-
 malonyl-CoA pH 6.8, recombinant wild-type enzyme Streptomyces coelicolor
2.3.1.39 0.023
-
 malonyl-CoA pH 6.8, recombinant mutant M126I Streptomyces coelicolor
2.3.1.39 0.032
-
 malonyl-CoA pH 6.8, recombinant mutant V98Q Streptomyces coelicolor
2.3.1.39 0.034
-
 malonyl-CoA pH 6.8, recombinant mutant A197D Streptomyces coelicolor
2.3.1.39 0.05
-
 malonyl-CoA pH 6.8, recombinant mutant F200S Streptomyces coelicolor
2.3.1.39 0.06
-
 malonyl-CoA pH 6.8, recombinant mutants G198Y and G198A Streptomyces coelicolor
2.3.1.39 0.075
-
 [acyl-carrier protein] pH 6.8, recombinant mutant F200Y Streptomyces coelicolor
2.3.1.39 0.083
-
 malonyl-CoA pH 6.8, recombinant mutant F200A Streptomyces coelicolor
2.3.1.39 0.095
-
 malonyl-CoA pH 6.8, recombinant mutant F200Y Streptomyces coelicolor
2.3.1.39 0.1
-
 [acyl-carrier protein] pH 6.8, recombinant mutants M137L and F200S Streptomyces coelicolor
2.3.1.39 0.12
-
 [acyl-carrier protein] pH 6.8, recombinant wild-type enzyme and mutant V98Q Streptomyces coelicolor
2.3.1.39 0.13
-
 [acyl-carrier protein] pH 6.8, recombinant mutants A197D and G198Y Streptomyces coelicolor
2.3.1.39 0.15
-
 malonyl-CoA pH 6.8, recombinant mutant R122A Streptomyces coelicolor
2.3.1.39 0.15
-
 [acyl-carrier protein] pH 6.8, recombinant mutants M126I and G198A Streptomyces coelicolor
2.3.1.39 0.2
-
 malonyl-CoA pH 6.8, recombinant mutant Q9A Streptomyces coelicolor
2.3.1.39 0.24
-
 [acyl-carrier protein] pH 6.8, recombinant mutant F200A Streptomyces coelicolor
2.3.1.39 0.4
-
 malonyl-CoA pH 6.8, recombinant mutant T57V Streptomyces coelicolor
2.3.1.39 0.57
-
 [acyl-carrier protein] pH 6.8, recombinant mutant T57V Streptomyces coelicolor
2.3.1.39 0.7
-
 malonyl-CoA pH 6.8, recombinant mutant R122K Streptomyces coelicolor
2.3.1.39 0.92
-
 [acyl-carrier protein] pH 6.8, recombinant mutant Q9A Streptomyces coelicolor
2.3.1.39 1.1
-
 [acyl-carrier protein] pH 6.8, recombinant mutant R122A Streptomyces coelicolor
2.3.1.39 3.5
-
 [acyl-carrier protein] pH 6.8, recombinant mutant R122K Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.39 malonyl-CoA + [acyl-carrier protein] Streptomyces coelicolor enzyme provides acyl-ACP thioesters for the biosynthesis of aromatic polyketides, overview, and thus is the primary gatekeeper of substrate specificity in type II polyketide synthase CoA + malonyl-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Streptomyces coelicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) Streptomyces coelicolor

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] putative active site structure and involved catalytic residues Streptomyces coelicolor
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.39 additional information
-
-
 Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + [acyl-carrier protein] enzyme provides acyl-ACP thioesters for the biosynthesis of aromatic polyketides, overview, and thus is the primary gatekeeper of substrate specificity in type II polyketide synthase Streptomyces coelicolor CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + [acyl-carrier protein] the active site is rigorously ordered around the acyl-thioester moiety of the acyl-CoA to facilitate rapid and efficient transacylation to an ACP Streptomyces coelicolor CoA + malonyl-[acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.39 malonyl-CoA:acyl carrier protein transacylase
-
 Streptomyces coelicolor
2.3.1.39 MAT
-
 Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.39 0.15
-
 malonyl-CoA pH 6.8, recombinant mutant F200S Streptomyces coelicolor
2.3.1.39 0.3
-
 malonyl-CoA pH 6.8, recombinant mutant M137L Streptomyces coelicolor
2.3.1.39 0.35
-
 malonyl-CoA pH 6.8, recombinant mutant R122A Streptomyces coelicolor
2.3.1.39 0.4
-
 malonyl-CoA pH 6.8, recombinant mutants G198Y Streptomyces coelicolor
2.3.1.39 0.7
-
 malonyl-CoA pH 6.8, recombinant mutant Q9A Streptomyces coelicolor
2.3.1.39 0.94
-
 malonyl-CoA pH 6.8, recombinant mutant F200A Streptomyces coelicolor
2.3.1.39 3 6 malonyl-CoA pH 6.8, recombinant mutant F200Y Streptomyces coelicolor
2.3.1.39 3
-
 malonyl-CoA pH 6.8, recombinant mutant M126I Streptomyces coelicolor
2.3.1.39 11
-
 malonyl-CoA pH 6.8, recombinant mutant R122K Streptomyces coelicolor
2.3.1.39 12
-
 malonyl-CoA pH 6.8, recombinant mutants G198A Streptomyces coelicolor
2.3.1.39 25
-
 malonyl-CoA pH 6.8, recombinant mutant T57V Streptomyces coelicolor
2.3.1.39 70
-
 malonyl-CoA pH 6.8, recombinant mutant A197D Streptomyces coelicolor
2.3.1.39 95
-
 malonyl-CoA pH 6.8, recombinant mutant V98Q Streptomyces coelicolor
2.3.1.39 100
-
 malonyl-CoA pH 6.8, recombinant wild-type enzyme Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.39 6.8
-
 assay at Streptomyces coelicolor