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Literature summary extracted from

  • Coombe, R.G.; George, A.M.
    Purification and properties of an aminoglycoside acetyltransferase from Pseudomonas aeruginosa (1982), Biochemistry, 21, 871-875.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.81 enzyme activity is significantly stabilized when preparations contain substrate Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.81 kanamycin A substrate inhibition Pseudomonas aeruginosa
2.3.1.81 tobramycin substrate inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.81 0.000724
-
 sisomicin pH 7.2, 34°C Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.81 39000
-
-
 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.81 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.81
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.81 additional information
-
 Vmax 0.102 (sisomicin), pH 7.2, 34°C Pseudomonas aeruginosa
2.3.1.81 9.743
-
 pH 7.2, 34°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.81 acetyl-CoA + sisomicin
-
 Pseudomonas aeruginosa CoA + N3'-acetylsisomicin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.81 AAC(3)
-
 Pseudomonas aeruginosa
2.3.1.81 aminoglycoside 3-acetyltransferase
-
 Pseudomonas aeruginosa