BRENDA - Enzyme Database show

Activity profiles of deoxynucleoside kinases and 5'-nucleotidases in cultured adipocytes and myoblastic cells: insights into mitochondrial toxicity of nucleoside analogs

Rylova, S.N.; Albertioni, F.; Flygh, G.; Eriksson, S.; Biochem. Pharmacol. 69, 951-960 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.74
quantitative real-time-PCR
Homo sapiens
2.7.1.113
quantitative real-time-PCR
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Homo sapiens
5829
-
2.7.1.113
mitochondrion
-
Homo sapiens
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
2.7.1.113
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
adipocyte
-
Homo sapiens
-
2.7.1.74
CEM cell
proliferating lymphocyte
Homo sapiens
-
2.7.1.74
lymphocyte
-
Homo sapiens
-
2.7.1.113
adipocyte
-
Homo sapiens
-
2.7.1.113
CEM cell
proliferating lymphocyte
Homo sapiens
-
2.7.1.113
lymphocyte
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.0000057
-
differentiated adipocytes
Homo sapiens
2.7.1.74
0.0000521
-
CEM lymphocytes
Homo sapiens
2.7.1.113
0.0000032
-
CEM lymphocytes
Homo sapiens
2.7.1.113
0.0000635
-
differentiated adipocytes
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
661040
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
661040
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
7.6
-
assay at
Homo sapiens
2.7.1.113
7.6
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.113
ATP
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
quantitative real-time-PCR
Homo sapiens
2.7.1.113
quantitative real-time-PCR
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.113
ATP
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Homo sapiens
5829
-
2.7.1.113
mitochondrion
-
Homo sapiens
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
adipocyte
-
Homo sapiens
-
2.7.1.74
CEM cell
proliferating lymphocyte
Homo sapiens
-
2.7.1.74
lymphocyte
-
Homo sapiens
-
2.7.1.113
adipocyte
-
Homo sapiens
-
2.7.1.113
CEM cell
proliferating lymphocyte
Homo sapiens
-
2.7.1.113
lymphocyte
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
0.0000057
-
differentiated adipocytes
Homo sapiens
2.7.1.74
0.0000521
-
CEM lymphocytes
Homo sapiens
2.7.1.113
0.0000032
-
CEM lymphocytes
Homo sapiens
2.7.1.113
0.0000635
-
differentiated adipocytes
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
661040
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
661040
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
7.6
-
assay at
Homo sapiens
2.7.1.113
7.6
-
assay at
Homo sapiens