BRENDA - Enzyme Database show

Anabolism of amdoxovir: phosphorylation of dioxolane guanosine and its 5'-phosphates by mammalian phosphotransferases

Feng, J.Y.; Parker, W.B.; Krajewski, M.L.; Deville-Bonne, D.; Veron, M.; Krishnan, P.; Cheng, Y.C.; Borroto-Esoda, K.; Biochem. Pharmacol. 68, 1879-1888 (2004)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.113
0.0017
-
2'-deoxyguanosine
recombinant enzyme, pH 7.6, 37°C
Homo sapiens
2.7.1.113
7
-
dioxolane guanosine
recombinant enzyme, pH 7.6, 37°C
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.113
ATP + dioxolane guanosine
Homo sapiens
low activity, step in anabolism of amdoxovir, i.e. (-)-beta-D-2,6-diaminopurine dioxolane, a prodrug of dioxolane guanosine
ADP + dioxolane guanosine 5'-phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
2.7.1.113
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
661034
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
dioxolane guanosine is no substrate
661034
Homo sapiens
?
-
-
-
-
2.7.1.113
ATP + 2'-deoxyadenosine
-
661034
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
661034
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + dioxolane guanosine
low activity, step in anabolism of amdoxovir, i.e. (-)-beta-D-2,6-diaminopurine dioxolane, a prodrug of dioxolane guanosine
661034
Homo sapiens
ADP + dioxolane guanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + dioxolane guanosine
0.02% of the activity with 2'-deoxyguanosine
661034
Homo sapiens
ADP + dioxolane guanosine 5'-phosphate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.113
37
-
assay at
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.113
0.025
-
dioxolane guanosine
recombinant enzyme, pH 7.6, 37°C
Homo sapiens
2.7.1.113
0.03
-
2'-deoxyguanosine
recombinant enzyme, pH 7.6, 37°C
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.113
7.6
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.113
ATP
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.113
ATP
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.113
0.0017
-
2'-deoxyguanosine
recombinant enzyme, pH 7.6, 37°C
Homo sapiens
2.7.1.113
7
-
dioxolane guanosine
recombinant enzyme, pH 7.6, 37°C
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
?
2.7.1.113
ATP + dioxolane guanosine
Homo sapiens
low activity, step in anabolism of amdoxovir, i.e. (-)-beta-D-2,6-diaminopurine dioxolane, a prodrug of dioxolane guanosine
ADP + dioxolane guanosine 5'-phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2'-deoxycytidine
-
661034
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
dioxolane guanosine is no substrate
661034
Homo sapiens
?
-
-
-
-
2.7.1.113
ATP + 2'-deoxyadenosine
-
661034
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
661034
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + dioxolane guanosine
low activity, step in anabolism of amdoxovir, i.e. (-)-beta-D-2,6-diaminopurine dioxolane, a prodrug of dioxolane guanosine
661034
Homo sapiens
ADP + dioxolane guanosine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + dioxolane guanosine
0.02% of the activity with 2'-deoxyguanosine
661034
Homo sapiens
ADP + dioxolane guanosine 5'-phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.113
37
-
assay at
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.113
0.025
-
dioxolane guanosine
recombinant enzyme, pH 7.6, 37°C
Homo sapiens
2.7.1.113
0.03
-
2'-deoxyguanosine
recombinant enzyme, pH 7.6, 37°C
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.113
7.6
-
assay at
Homo sapiens