Any feedback?
Literature summary extracted from
- Microsomal cholesterol epoxide hydrolase activity in 2-acetylaminofluorene-induced rat liver hyperplastic nodules (1987), Biochem. Pharmacol., 36, 2424-2427.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.3.2.11 | acetonitrile | maximal enzyme activity at 6.25% v/v | Rattus norvegicus |  |
| 3.3.2.11 | additional information | 2-acetylaminofluorene induces the enzyme | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.3.2.11 | 9-fluorenone | slight inhibition | Rattus norvegicus | |
| 3.3.2.11 | chalcone | - |
Rattus norvegicus | |
| 3.3.2.11 | ellipticine | - |
Rattus norvegicus | |
| 3.3.2.11 | harman | - |
Rattus norvegicus | |
| 3.3.2.11 | Isoquinoline | slight inhibition | Rattus norvegicus | |
| 3.3.2.11 | Metyrapone | weak inhibition in hyperplastic nodules, not in normal tissue | Rattus norvegicus | |
| 3.3.2.11 | additional information | comparison of inhibition level in microsomes of normal liver tissue and liver hyperplastic nodules, remaining activities, overview | Rattus norvegicus | |
| 3.3.2.11 | norharman | - |
Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.3.2.11 | 0.00091 | - |
5,6-epoxy-5alpha-cholestan-3beta-ol | pH 7.4, 37°C, normal liver tissue microsomes | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.3.2.11 | microsome | - |
Rattus norvegicus | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.3.2.11 | Rattus norvegicus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.3.2.11 | liver | normal tissue and hyperplastic nodules | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.11 | 0.00007 | - |
microsomes from hyperplastic nodules | Rattus norvegicus |
| 3.3.2.11 | 0.00017 | - |
microsomes from normal liver tissue | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.11 | 5,6-epoxy-5alpha-cholestan-3beta-ol + H2O | - |
Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.3.2.11 | cholesterol epoxide hydrolase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.11 | 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.11 | 7.4 | - |
assay at | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
