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Literature summary extracted from
- A novel assay method for an amino acid racemase reaction based on circular dichroism (2005), Biochem. J., 389, 491-496.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.1 | 0.25 | - |
D-alanine | pH 8.2, 37°C | Escherichia coli |  |
| 5.1.1.1 | 0.29 | - |
L-alanine | pH 8.2, 37°C | Escherichia coli | |
| 5.1.1.1 | 0.4 | - |
L-alanine | pH 8.2, 37°C | Streptomyces lavendulae | |
| 5.1.1.1 | 0.4 | - |
D-alanine | pH 8.2, 37°C | Streptomyces lavendulae | |
| 5.1.1.1 | 10 | - |
D-serine | pH 8.2, 37°C | Streptomyces lavendulae | |
| 5.1.1.1 | 27 | - |
L-serine | pH 8.2, 37°C | Streptomyces lavendulae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.1 | Escherichia coli | - |
- |
- |
| 5.1.1.1 | Streptomyces lavendulae | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.1 | additional information | - |
assay method based on circular dichroism spectra of D- and L-alanine | Escherichia coli |
| 5.1.1.1 | additional information | - |
assay method based on circular dichroism spectra of D- and L-alanine | Streptomyces lavendulae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.1 | D-alanine | - |
Escherichia coli | L-alanine | - |
r | |
| 5.1.1.1 | D-alanine | - |
Streptomyces lavendulae | L-alanine | - |
r | |
| 5.1.1.1 | D-serine | - |
Streptomyces lavendulae | L-serine | - |
r | |
| 5.1.1.1 | L-alanine | - |
Escherichia coli | D-alanine | - |
r | |
| 5.1.1.1 | L-alanine | - |
Streptomyces lavendulae | D-alanine | - |
r | |
| 5.1.1.1 | L-serine | - |
Streptomyces lavendulae | D-serine | - |
r | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.1 | 2 | 8 | D-alanine | pH 8.2, 37°C | Escherichia coli | |
| 5.1.1.1 | 5.5 | - |
D-serine | pH 8.2, 37°C | Streptomyces lavendulae | |
| 5.1.1.1 | 13.3 | - |
L-serine | pH 8.2, 37°C | Streptomyces lavendulae | |
| 5.1.1.1 | 27.6 | - |
L-alanine | pH 8.2, 37°C | Escherichia coli | |
| 5.1.1.1 | 55 | - |
L-alanine | pH 8.2, 37°C | Streptomyces lavendulae | |
| 5.1.1.1 | 63.3 | - |
D-alanine | pH 8.2, 37°C | Streptomyces lavendulae | |
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Release 2023.1 (January 2023)
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