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Literature summary extracted from

  • Datta, R.; Das, I.; Sen, B.; Chakraborty, A.; Adak, S.; Mandal, C.; Datta, A.K.
    Mutational analysis of the active-site residues crucial for catalytic activity of adenosine kinase from Leishmania donovani (2005), Biochem. J., 387, 591-600.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.20 expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain M15 Leishmania donovani

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.20 D299A site-directed mutagenesis, inactive mutant, slightly reduced AMP binding ability compared to the wild-type enzyme Leishmania donovani
2.7.1.20 G62D site-directed mutagenesis, inactive mutant, no AMP binding ability Leishmania donovani
2.7.1.20 R131A site-directed mutagenesis, inactive mutant, reduced catalytic activity and highly reduced AMP binding ability compared to the wild-type enzyme Leishmania donovani
2.7.1.20 R69A site-directed mutagenesis, reduced reduced catalytic activity and AMP binding ability compared to the wild-type enzyme Leishmania donovani
2.7.1.20 R69K site-directed mutagenesis, reduced catalytic activity and slightly reduced AMP binding ability compared to the wild-type enzyme Leishmania donovani
2.7.1.20 R69K/R131A site-directed mutagenesis, highly reduced catalytic activity and AMP binding ability compared to the wild-type enzyme Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 Phenylglyoxal inhibition levels of wild-type and mutant enzymes, overview Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 0.015
-
 adenosine pH 7.5, 30°C, recombinant mutant R69A Leishmania donovani
2.7.1.20 0.016
-
 adenosine pH 7.5, 30°C, recombinant mutants R131A and R69K/R131A Leishmania donovani
2.7.1.20 0.017
-
 adenosine pH 7.5, 30°C, recombinant mutant R69K Leishmania donovani
2.7.1.20 0.02
-
 adenosine pH 7.5, 30°C, recombinant wild-type enzyme Leishmania donovani
2.7.1.20 0.042
-
 ATP pH 7.5, 30°C, recombinant wild-type enzyme Leishmania donovani
2.7.1.20 0.068
-
 ATP pH 7.5, 30°C, recombinant mutant R69A Leishmania donovani
2.7.1.20 0.071
-
 ATP pH 7.5, 30°C, recombinant mutant R131A Leishmania donovani
2.7.1.20 0.278
-
 ATP pH 7.5, 30°C, recombinant mutant R69K Leishmania donovani
2.7.1.20 1.282
-
 ATP pH 7.5, 30°C, recombinant mutant R69K/R131A Leishmania donovani

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Mg2+ at 1.5 mM MgCl2 Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.20 ATP + adenosine Leishmania donovani
-
 ADP + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Leishmania donovani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography Leishmania donovani

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.20 ATP + adenosine = ADP + AMP residues G62, D299, R69, and R131 are crucial for catalytic activity Leishmania donovani
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + adenosine
-
 Leishmania donovani ADP + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.20 More modeling of the three-dimensional structures of wild-type and mutant enzymes, overview Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
2.7.1.20 ADK
-
 Leishmania donovani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.20 30
-
 assay at Leishmania donovani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.20 0.006
-
 adenosine pH 7.5, 30°C, recombinant mutant R69K/R131A Leishmania donovani
2.7.1.20 0.008
-
 ATP pH 7.5, 30°C, recombinant mutant R69K/R131A Leishmania donovani
2.7.1.20 0.029
-
 ATP pH 7.5, 30°C, recombinant mutant R69A Leishmania donovani
2.7.1.20 0.032
-
 ATP pH 7.5, 30°C, recombinant mutant R69K Leishmania donovani
2.7.1.20 0.038
-
 adenosine pH 7.5, 30°C, recombinant mutant R69A Leishmania donovani
2.7.1.20 0.043
-
 adenosine pH 7.5, 30°C, recombinant mutant R69K Leishmania donovani
2.7.1.20 0.052
-
 ATP pH 7.5, 30°C, recombinant mutant R131A Leishmania donovani
2.7.1.20 0.065
-
 adenosine pH 7.5, 30°C, recombinant mutant R131A Leishmania donovani
2.7.1.20 1.2
-
 ATP pH 7.5, 30°C, recombinant wild-type enzyme Leishmania donovani
2.7.1.20 1.4
-
 adenosine pH 7.5, 30°C, recombinant wild-type enzyme Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.20 7.5
-
 assay at Leishmania donovani