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Literature summary extracted from

  • Gul, S.; Brown, R.; May, E.; Mazzulla, M.; Smyth, M.G.; Berry, C.; Morby, A.; Powell, D.J.
    Staphylococcus aureus DNA ligase: characterization of its kinetics of catalysis and development of a high-throughput screening compatible chemiluminescent hybridization protection assay (2004), Biochem. J., 383, 551-559.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2 expression in Escherichia coli Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.2 Chloroquine diphosphate IC50: 0.0014 mM Staphylococcus aureus
6.5.1.2 doxorubicin IC50: 0.0033 mM Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.2 0.0000025
-
 acridinium-ester labelled DNA pH 7.4, 21°C Staphylococcus aureus
6.5.1.2 0.00275
-
 NAD+ pH 7.4, 21°C Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 ATP + acridinium-ester labelled DNA
-
 Staphylococcus aureus ?
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m acridinium-ester labelled DNA Staphylococcus aureus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 NAD+
-
 Staphylococcus aureus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.5.1.2 0.0014
-
 IC50: 0.0014 mM Staphylococcus aureus Chloroquine diphosphate
6.5.1.2 0.0033
-
 IC50: 0.0033 mM Staphylococcus aureus doxorubicin