Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Weatherly, S.C.; Volrath, S.L.; Elich, T.D.
    Expression and characterization of recombinant fungal acetyl-CoA carboxylase and isolation of a soraphen-binding domain (2004), Biochem. J., 380, 105-110.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.2 biotin
-
 Pyricularia grisea
6.4.1.2 biotin
-
 Phytophthora infestans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.14 truncated biotin carboxylase domain of acetyl-CoA carboxylase, expression in Escherichia coli Ustilago maydis
6.3.4.14 truncated biotin carboxylase domain of acetyl-CoA carboxylase, expression in Escherichia coli Pyricularia grisea
6.3.4.14 truncated biotin carboxylase domain of acetyl-CoA carboxylase, expression in Escherichia coli Phytophthora infestans
6.4.1.2 expression of the BC domains in Escherichia coli strain BL21(DE3) Pyricularia grisea
6.4.1.2 expression of the BC domains in Escherichia coli strain BL21(DE3) Phytophthora infestans
6.4.1.2 functional expression of the full length enzyme, and expression of the His-tagged BC domain in Escherichia coli Ustilago maydis

General Stability

EC Number General Stability Organism
6.3.4.14 the truncated biotin carboxylase domain is more stable than the full-length enzyme Ustilago maydis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.14 soraphen
-
 Phytophthora infestans
6.3.4.14 soraphen
-
 Pyricularia grisea
6.3.4.14 soraphen
-
 Ustilago maydis
6.4.1.2 soraphen A noncompetitive, binds to the BC domain Phytophthora infestans
6.4.1.2 soraphen A noncompetitive, binds to the BC domain Pyricularia grisea
6.4.1.2 soraphen A noncompetitive, binds to the BC domain Ustilago maydis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 0.14
-
 acetyl-CoA pH 8.0, 30°C, recombinant enzyme Ustilago maydis
6.4.1.2 0.19
-
 ATP pH 8.0, 30°C, recombinant enzyme Ustilago maydis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.2 Mg2+
-
 Pyricularia grisea
6.4.1.2 Mg2+
-
 Ustilago maydis
6.4.1.2 Mg2+
-
 Phytophthora infestans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.14 64700
-
 x * 64700, recombinant biotin carboxylase domain Ustilago maydis
6.4.1.2 65000
-
 x * 65000, recombinant BC domain, SDS-PAGE Ustilago maydis
6.4.1.2 66000
-
 recombinant BC domain, gel filtration Ustilago maydis
6.4.1.2 225000
-
 4 * 225000, recombinant enzyme, SDS-PAGE Ustilago maydis
6.4.1.2 880000
-
 approximately, recombinant enzyme, gel filtration Ustilago maydis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3- Pyricularia grisea
-
 ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- Phytophthora infestans
-
 ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- Ustilago maydis the enzyme catalyzes the first step in fatty acid bioynthesis ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- Pyricularia grisea Guy11
-
 ADP + malonyl-CoA + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.14 Phytophthora infestans
-
-
-
6.3.4.14 Pyricularia grisea
-
-
-
6.3.4.14 Ustilago maydis
-
-
-
6.4.1.2 Phytophthora infestans Q6SZP8 genotype US-1
-
6.4.1.2 Pyricularia grisea
-
-
-
6.4.1.2 Pyricularia grisea Guy11
-
-
-
6.4.1.2 Ustilago maydis Q12721
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
6.4.1.2 additional information the enzyme is biotinylated Pyricularia grisea
6.4.1.2 additional information the enzyme is biotinylated Ustilago maydis
6.4.1.2 additional information the enzyme is biotinylated Phytophthora infestans

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2 recombinant full length enzyme and His-tagged BC domain from Escherichia coli, the latter by nickel affinity chromatography Ustilago maydis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 0.3
-
 purified recombinant enzyme Ustilago maydis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Pyricularia grisea ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Ustilago maydis ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Phytophthora infestans ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- the enzyme catalyzes the first step in fatty acid bioynthesis Ustilago maydis ADP + malonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Pyricularia grisea Guy11 ADP + malonyl-CoA + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.14 ? x * 64700, recombinant biotin carboxylase domain Ustilago maydis
6.4.1.2 ? x * 65000, recombinant BC domain, SDS-PAGE Ustilago maydis
6.4.1.2 tetramer 4 * 225000, recombinant enzyme, SDS-PAGE Ustilago maydis

Synonyms

EC Number Synonyms Comment Organism
6.4.1.2 ACC
-
 Pyricularia grisea
6.4.1.2 ACC
-
 Ustilago maydis
6.4.1.2 ACC
-
 Phytophthora infestans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.2 30
-
 assay at Pyricularia grisea
6.4.1.2 30
-
 assay at Ustilago maydis
6.4.1.2 30
-
 assay at Phytophthora infestans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 8
-
 assay at Pyricularia grisea
6.4.1.2 8
-
 assay at Ustilago maydis
6.4.1.2 8
-
 assay at Phytophthora infestans

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.2 ATP
-
 Pyricularia grisea
6.4.1.2 ATP
-
 Ustilago maydis
6.4.1.2 ATP
-
 Phytophthora infestans
6.4.1.2 biotin covalently bound prosthetic group, required for activity Ustilago maydis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.4.1.2 additional information
-
 additional information soraphen A dissociation constant is 0.8 nM Ustilago maydis
6.4.1.2 0.0000021
-
 soraphen A recombinant enzyme, pH 8.0, 30°C Ustilago maydis