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Literature summary extracted from

  • Giudici, M.L.; Emson, P.C.; Irvine, R.F.
    A novel neuronal-specific splice variant of Type I phosphatidylinositol 4-phosphate 5-kinase isoform gamma (2004), Biochem. J., 379, 489-496.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.68 identification of splicing form c of type I phosphatidylinositol 4-phosphate 5-kinase isoform gamma possessing additional 26 amino acids at the C-terminus from a hippocampus library, DNA and amino acid sequence determination and analysis, overvexpression of the catalytically inactive mutants D316K and K188A of isozyme gamma splicing form c in rat cerebellar granule cells, expression of isozyme gamma splicing form a, expression of wild-type and FLAG-tagged isozyme gamma splicing form c in COS-7 cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.68 D316K site-directed mutagenesis, overexpression of the catalytically inactive construct of isozyme gamma splicing form c in rat cerebellar granule cells causes progressive loss of their neuronal processes, not observed with expression of isozyme gamma splicing form a Rattus norvegicus
2.7.1.68 K188A site-directed mutagenesis, overexpression of the catalytically inactive construct of isozyme gamma splicing form c in rat cerebellar granule cells causes progressive loss of their neuronal processes, not observed with expression of isozyme gamma splicing form a Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.68 KCl at 80 mM Rattus norvegicus
2.7.1.68 MgCl2 at 10 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.68 75600
-
 x * 75600, isozyme splicing form c, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Rattus norvegicus
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
2.7.1.68 additional information Rattus norvegicus splicing form a of the isozyme gamma is inactive, splicing form c is involved in maintenance of some neuronal cellular processes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.68 Rattus norvegicus
-
 type I phosphatidylinositol 4-phosphate 5-kinase isozyme gamma, 3 splicing forms a-c
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.68 wild-type and mutant isozyme gamma, partially by immunoprecipitation and protein G affinity chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.68 brain
-
 Rattus norvegicus
-
2.7.1.68 cerebellum granule cells Rattus norvegicus
-
2.7.1.68 hippocampus dentate gyrus, pyramidal cells Rattus norvegicus
-
2.7.1.68 neuron in the pyramidal cells of the hippocampus and of the cerebral cortex, splicing form c of isozyme gamma is neuronal-specific Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
2.7.1.68 additional information splicing form a of the isozyme gamma is inactive, splicing form c is involved in maintenance of some neuronal cellular processes Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.68 ? x * 75600, isozyme splicing form c, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.68 phosphatidylinositol 4-phosphate 5-kinase
-
 Rattus norvegicus
2.7.1.68 PIPkin
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.68 30
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.68 7.4
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.68 ATP
-
 Rattus norvegicus