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Literature summary extracted from

  • Abe, T.; Lu, X.; Jiang, Y.; Boccone, C.E.; Qian, S.; Vattem, K.M.; Wek, R.C.; Walsh, J.P.
    Site-directed mutagenesis of the active site of diacylglycerol kinase alpha: calcium and phosphatidylserine stimulate enzyme activity via distinct mechanisms (2003), Biochem. J., 375, 673-680.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.107 acidic phospholipids activation in vitro Sus scrofa
2.7.1.107 phosphatidylserine 10-20 mol% result in 7.5-7.8fold activation of the recombinant wild-type enzyme, 3.8fold of the recombinant mutant DELTA196, and 6.5fold of the recombinant mutant DELTA332 Sus scrofa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 expression of wild-type and deletion mutant enzymes in COS-1 cells, expression of wild-type and point mutant enzymes in the enzyme-deficient Saccharomyces cerevisiae strain WY294 Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.107 D434A site-directed mutagenesis, inactive mutant Sus scrofa
2.7.1.107 D434N site-directed mutagenesis, inactive mutant Sus scrofa
2.7.1.107 D465A site-directed mutagenesis, inactive mutant Sus scrofa
2.7.1.107 D465N site-directed mutagenesis, 0.1% of wild-type activity Sus scrofa
2.7.1.107 D497A site-directed mutagenesis, inactive mutant Sus scrofa
2.7.1.107 D497N site-directed mutagenesis, 0.9% of wild-type activity, reduced stimulation by Ca2+ and phosphatidylserine compared to the wild-type enzyme Sus scrofa
2.7.1.107 D529A site-directed mutagenesis, 1.1% of wild-type activity Sus scrofa
2.7.1.107 D529N site-directed mutagenesis, 5.5% of wild-type activity, unaltered stimulation by Ca2+ and phosphatidylserine compared to the wild-type enzyme Sus scrofa
2.7.1.107 D650A site-directed mutagenesis, inactive mutant Sus scrofa
2.7.1.107 D650N site-directed mutagenesis, inactive mutant Sus scrofa
2.7.1.107 D697A site-directed mutagenesis, 1.4% of wild-type activity Sus scrofa
2.7.1.107 D697N site-directed mutagenesis, 4.0% of wild-type activity, reduced stimulation by Ca2+ and phosphatidylserine compared to the wild-type enzyme Sus scrofa
2.7.1.107 additional information construction of deletion mutants DELTA196, lacking the RVH motif and the EF hand, and DELTA332, lacking the RVH motif, the EF hand, and the C1 domain, mutant DELTA332 shows 50% of wild-type activity Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 membrane
-
 Sus scrofa 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Ca2+ activates in vitro, interacts via N-terminal RVH motif and EF hands Sus scrofa
2.7.1.107 Mg2+ coordidating bivalent metal ion is involved in substrate binding Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol Sus scrofa reaction takes place during stimulated phosphatidylinositol turnover ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 partially, recombinant wild-type and point mutant enzymes from yeast by ion exchange chromatography Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate C-terminal active site comprises residues 371-501, the C1 domain is absolutely required for catalytic activity, residues Asp434, Asp650, Asp465, and Asp497 are involved Sus scrofa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol
-
 Sus scrofa ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1,2-diacylglycerol reaction takes place during stimulated phosphatidylinositol turnover Sus scrofa ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.107 DAGK
-
 Sus scrofa
2.7.1.107 diacylglycerol kinase alpha
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.107 ATP
-
 Sus scrofa