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Literature summary extracted from

  • Monasterio, O.; Cardenas, M.L.
    Kinetic studies of rat liver hexokinase D ('glucokinase') in non-co-operative conditions show an ordered mechanism with MgADP as the last product to be released (2003), Biochem. J., 371, 29-38.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 2-deoxy-D-glucose low substrate inhibition Rattus norvegicus
2.7.1.1 5,5'-dithiobis-(2-nitrobenzoic acid) inactivation, protection by MgADP-, AMP, 2-deoxyglucose, glucose, and mannose probably via binary complex formation, no protection by glucose 6-phosphate, slight protection by MgATP2- Rattus norvegicus
2.7.1.1 AMP binds to free enzyme and to D-glucose-enzyme Rattus norvegicus
2.7.1.1 Cr(III)-ATP complex of ATP with chromium in the 3+ oxidation state, mixed versus MgATP2-, competitive inhibition versus 2-deoxyglucose Rattus norvegicus
2.7.1.1 MgADP- product inhibition, mixed type inhibition versus MgATP2-, competitive inhibition versus 2-deoxyglucose Rattus norvegicus
2.7.1.1 additional information inhibition mechanism, overview Rattus norvegicus
2.7.1.1 N-acetylglucosamine mixed versus MgATP2-, competitive inhibition versus 2-deoxyglucose Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 additional information
-
additional information non-cooperative conditions shows an ordered, ternary-complex mechanism with MgADP- as the last product to be released, hyperbolic kinetics with both 2-deoxyglucose and MgATP2- Rattus norvegicus
2.7.1.1 0.56
-
ATP pH 8.0, 30°C Rattus norvegicus
2.7.1.1 0.56
-
ATP ATP in form of MgATP2- Rattus norvegicus
2.7.1.1 19.2
-
2-deoxy-D-glucose pH 8.0, 30°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.1 Mg2+
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 ATP + D-glucose Rattus norvegicus
-
ADP + D-glucose 6-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Rattus norvegicus
-
male Wistar rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 from liver, to homogeneity Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.1 ATP + D-hexose = ADP + D-hexose 6-phosphate non-cooperative conditions shows an ordered kinetic mechanism with MgADP as the last product to be released Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 1.5
-
partially purified liver enzyme Rattus norvegicus
2.7.1.1 70
-
purified liver enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose
-
Rattus norvegicus ADP + 2-deoxy-D-glucose 6-phosphate
-
?
2.7.1.1 ATP + D-glucose
-
Rattus norvegicus ADP + D-glucose 6-phosphate
-
?
2.7.1.1 ATP + D-hexose
-
Rattus norvegicus ADP + D-hexose 6-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.1 glucokinase
-
Rattus norvegicus
2.7.1.1 hexokinase D
-
Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 30
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 8
-
assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP formation of MgATP2- before reaction Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.1 additional information
-
additional information inhibition kinetics, overview Rattus norvegicus
2.7.1.1 0.63
-
N-acetylglucosamine pH 8.0, 30°C, versus 2-deoxyglucose Rattus norvegicus
2.7.1.1 0.8
-
N-acetylglucosamine pH 8.0, 30°C, versus MgATP2- Rattus norvegicus
2.7.1.1 4.5
-
Cr(III)-ATP pH 6.9, 30°C, versus MgATP2- Rattus norvegicus
2.7.1.1 40.7
-
2-deoxy-D-glucose pH 8.0, 30°C, versus MgATP2- Rattus norvegicus