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Literature summary extracted from

  • Nalaskowski, M.M.; Deschermeier, C.; Fanick, W.; Mayr, G.W.
    The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization (2002), Biochem. J., 366, 549-556.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.151 DNA and amino acid sequence determination and analysis, overexpression as C-terminally GFP-tagged fusion protein in Escherichia coli strain BL21(DE3), transient expression in NRK 52E cells as C- or N-terminally GFP-tagged fusion protein Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.151 0.00028
-
 1D-myo-inositol 1,4,5-trisphosphate pH 7.8, 30°C, 0.5 mM ATP, recombinant enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.151 nucleus predominantly, a basic cluster at the enzyme's C-terminus is involved in nuclear targeting Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.151 KCl
-
 Homo sapiens
2.7.1.151 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.151 47219
-
 x * 47219, amino acid sequence calculation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.151 Homo sapiens Q8NFU5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.151 recombinant enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by ion exchange and phosphocellulose resin chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.151 ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate the catalytic site contains an important Ser-Ser-Leu-Leu motif Homo sapiens
a
2.7.1.151 ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate the catalytic site contains an important Ser-Ser-Leu-Leu motif Homo sapiens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.151 0.45
-
 purified recombinant enzyme, 1D-myo-inositol 1,3,4,5,6-pentakisphosphate formation from 1D-myo-inositol 1,4,5-trisphosphate, 0.5 mM ATP Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.151 2 ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Homo sapiens 2 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ir
2.7.1.151 ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.1.151 ? x * 47219, amino acid sequence calculation Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.151 inositol phosphate multikinase
-
 Homo sapiens
2.7.1.151 IPMK
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.151 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.151 7.8
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.151 ATP
-
 Homo sapiens