EC Number | Application | Comment | Organism |
---|---|---|---|
3.3.2.10 | synthesis | the enzyme is useful for enantioselective bio-organic synthesis of chiral substances | Aspergillus niger |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.3.2.10 | DNA and amino acid sequence determination and analysis, phylogenetic tree, sequence comparisons, functional expression of wild-type enzyme and expression of enzyme mutants in Escherichia coli strain BL21(DE3) | Aspergillus niger |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.3.2.10 | D192A | site directed mutagenesis, inactive or nearly inactive mutant | Aspergillus niger |
3.3.2.10 | D192N | site directed mutagenesis, inactive or nearly inactive mutant | Aspergillus niger |
3.3.2.10 | D192S | site directed mutagenesis, inactive or nearly inactive mutant | Aspergillus niger |
3.3.2.10 | D348A | site directed mutagenesis, inactive or nearly inactive mutant | Aspergillus niger |
3.3.2.10 | D348E | site directed mutagenesis, the mutant shows 48% of wild-type enzyme activity | Aspergillus niger |
3.3.2.10 | H374K | site directed mutagenesis, inactive or nearly inactive mutant | Aspergillus niger |
3.3.2.10 | H374N | site directed mutagenesis, inactive or nearly inactive mutant | Aspergillus niger |
3.3.2.10 | H374S | site directed mutagenesis, inactive or nearly inactive mutant, recombinantly expressed mutant enzyme is not soluble but remains in the particulate fraction of Escherichia coli cells | Aspergillus niger |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.3.2.10 | soluble | the enzyme has no N-terminal membrane anchor in contrast to the microsomal epoxide hydrolase, 3.3.2.9 | Aspergillus niger | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.3.2.10 | 100000 | - |
recombinant enzyme, gel filtration | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.3.2.10 | Aspergillus niger | Q9UR30 | - |
- |
3.3.2.10 | Aspergillus niger LCP521 | Q9UR30 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.3.2.10 | recombinant enzyme from Escherichia coli strain BL21(DE3) to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration | Aspergillus niger |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.3.2.10 | an epoxide + H2O = a glycol | the catalytic triad is formed by Asp192, Asp348, and His374 | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.3.2.10 | (R)-4-nitrostyrene oxide + H2O | - |
Aspergillus niger | ? | - |
? | |
3.3.2.10 | (R)-4-nitrostyrene oxide + H2O | - |
Aspergillus niger LCP521 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.3.2.10 | More | the enzyme has no N-terminal membrane anchor in contrast to the microsomal epoxide hydrolase, EC 3.3.2.9 | Aspergillus niger |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.3.2.10 | EH | - |
Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.3.2.10 | 37 | - |
assay at | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.3.2.10 | 7.4 | - |
assay at | Aspergillus niger |