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Literature summary extracted from

  • O'Day, D.H.; Chatterjee-Chakraborty, M.; Wagler, S.; Myre, M.A.
    Isolation and characterization of Dictyostelium thymidine kinase 1 as a calmodulin-binding protein (2005), Biochem. Biophys. Res. Commun., 331, 1494-1502.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.21 Calmodulin stimulates the enzyme by 13.8%, might modify the activity, enzyme contains a calmodulin binding domain, Ca2+ is required for binding, compounds W7 and W13 prevent stimulation, overview Dictyostelium discoideum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 gene thyB, DNA and amino acid sequence determination and analysis, phage display, location on chromosome 5, 3 exons, 2 introns, expression in Escherichia coli strain BL21 Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 W7 slight inhibitory in presence of calmodulin Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.21 cytosol
-
 Dictyostelium discoideum 5829
-
2.7.1.21 additional information enzyme possesses no nuclear or transmembrane sequences Dictyostelium discoideum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.21 Ca2+ calmodulin binding binding is dependent on Ca2+ Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 25494
-
 x * 25494, recombinant enzyme, amino acid sequence calculation Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.21 ATP + thymidine Dictyostelium discoideum
-
 ADP + thymidine 5'-phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Dictyostelium discoideum Q27564
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 recombinant enzyme partially utilizing calmodulin-agarose-beads Dictyostelium discoideum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.21 additional information enzyme is expressed during growth, but decreases in amount after starvation Dictyostelium discoideum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + thymidine
-
 Dictyostelium discoideum ADP + thymidine 5'-phosphate
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.1.21 ? x * 25494, recombinant enzyme, amino acid sequence calculation Dictyostelium discoideum
2.7.1.21 More enzyme contains a calmodulin binding domain Dictyostelium discoideum

Synonyms

EC Number Synonyms Comment Organism
2.7.1.21 calmodulin-binding protein
-
 Dictyostelium discoideum
2.7.1.21 CaM-binding protein
-
 Dictyostelium discoideum
2.7.1.21 CaMBP
-
 Dictyostelium discoideum
2.7.1.21 ThyB
-
 Dictyostelium discoideum
2.7.1.21 thymidine kinase 1
-
 Dictyostelium discoideum
2.7.1.21 TK1
-
 Dictyostelium discoideum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.21 37
-
 assay at Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.21 7.5
-
 assay at Dictyostelium discoideum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.21 ATP P-loop binding site Dictyostelium discoideum