BRENDA - Enzyme Database

Dibasic amino acid residues at the carboxy-terminal end of kinase homology domain participate in the plasma membrane localization and function of phosphatidylinositol 5-kinase gamma

Arioka, M.; Nakashima, S.; Shibasaki, Y.; Kitamoto, K.; Biochem. Biophys. Res. Commun. 319, 456-463 (2004)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.68
phosphatidic acid
activation
Mus musculus
2.7.1.68
small GTPase ARF/phosphatidic acid
regulation of isozyme gamma
Mus musculus
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.68
functional complementation of a yeast Mss4-1 mutant strain YOC808 by the wild-type isozyme gamma and the deletion mutants localizing to the plasma membrane, while the deletion mutants localized to the cytosol cannot support yeast cell growth, expression of wild-type and mutant type I HA-tagged or EGFP-tagged isozymes gamma in Neuro 2A cells and in COS-1 cells
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.68
additional information
construction of N-terminal deletion mutants DELTA68, DELTA 72, and DELTA74 are still localized to the plasma membrane, while the N-terminal deletion mutants DELTA82, DELTA 97 are mislocated to the cytosol, construction of C-terminal deletion mutant DELTA446 is still localized to the plasma membrane, while the N-terminal deletion mutant DELTA444 is mislocated to the cytosol, mutant fusion protein DELTA68-DELTA444 localizes to the plasma membrane
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.68
plasma membrane
type I isozyme gamma, the highly conserved central kinase domain KHD is necessary and sufficient for plasma membrane localization, with the C-termnal Arg-Lys dibasic sequence being responsible for plasma membrane targeting
Mus musculus
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.68
Mg2+
-
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Mus musculus
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is an important lipid in several cellular processes e.g. in actin cytoskeleton regulation and membrane trafficking
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.68
Mus musculus
-
type I enzymes, isozyme gamma
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660875
Mus musculus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is an important lipid in several cellular processes e.g. in actin cytoskeleton regulation and membrane trafficking
660875
Mus musculus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.68
ATP
-
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.68
phosphatidic acid
activation
Mus musculus
2.7.1.68
small GTPase ARF/phosphatidic acid
regulation of isozyme gamma
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.68
functional complementation of a yeast Mss4-1 mutant strain YOC808 by the wild-type isozyme gamma and the deletion mutants localizing to the plasma membrane, while the deletion mutants localized to the cytosol cannot support yeast cell growth, expression of wild-type and mutant type I HA-tagged or EGFP-tagged isozymes gamma in Neuro 2A cells and in COS-1 cells
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.68
ATP
-
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.68
additional information
construction of N-terminal deletion mutants DELTA68, DELTA 72, and DELTA74 are still localized to the plasma membrane, while the N-terminal deletion mutants DELTA82, DELTA 97 are mislocated to the cytosol, construction of C-terminal deletion mutant DELTA446 is still localized to the plasma membrane, while the N-terminal deletion mutant DELTA444 is mislocated to the cytosol, mutant fusion protein DELTA68-DELTA444 localizes to the plasma membrane
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.68
plasma membrane
type I isozyme gamma, the highly conserved central kinase domain KHD is necessary and sufficient for plasma membrane localization, with the C-termnal Arg-Lys dibasic sequence being responsible for plasma membrane targeting
Mus musculus
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.68
Mg2+
-
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Mus musculus
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is an important lipid in several cellular processes e.g. in actin cytoskeleton regulation and membrane trafficking
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660875
Mus musculus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate is an important lipid in several cellular processes e.g. in actin cytoskeleton regulation and membrane trafficking
660875
Mus musculus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?