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Literature summary extracted from

  • Kato, C.; Kurihara, T.; Kobashi, N.; Yamane, H.; Nishiyama, M.
    Conversion of feedback regulation in aspartate kinase by domain exchange (2004), Biochem. Biophys. Res. Commun., 316, 802-808.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.4 L-threonine enhances activity up to 140% at 0.2 mM Bacillus subtilis
2.7.2.4 L-threonine enhances activity up to 140% at 0.2 mM Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.4 expression in Escherichia coli Bacillus subtilis
2.7.2.4 expression in Escherichia coli Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 L-lysine no inhibition is observed; the truncated protein is no longer inhibited by lysine, even at 10 mM, wild type protein is inhibited by lysine Bacillus subtilis
2.7.2.4 L-lysine no inhibition is observed Thermus thermophilus
2.7.2.4 L-threonine almost insensitive to threonine Bacillus subtilis
2.7.2.4 L-threonine
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.4 3.9
-
 L-aspartate
-
 Bacillus subtilis
2.7.2.4 3.9
-
 L-aspartate
-
 Thermus thermophilus
2.7.2.4 4.5
-
 ATP
-
 Bacillus subtilis
2.7.2.4 4.5
-
 ATP
-
 Thermus thermophilus
2.7.2.4 6.5
-
 ATP wild type Bacillus subtilis
2.7.2.4 6.9
-
 ATP
-
 Bacillus subtilis
2.7.2.4 6.9
-
 ATP
-
 Thermus thermophilus
2.7.2.4 10
-
 L-aspartate wild type Bacillus subtilis
2.7.2.4 10.6
-
 L-aspartate
-
 Bacillus subtilis
2.7.2.4 10.6
-
 L-aspartate
-
 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.4 18000
-
 beta subunit, calculated from nucleotide sequence Thermus thermophilus
2.7.2.4 18000
-
 alpha2, beta2, 2 * 42700 + 2 * 18000, gel filtration, ultracentrifugation, calculated from nucleotide sequence Bacillus subtilis
2.7.2.4 18500
-
 beta subunit, calculated from nucleotide sequence Bacillus subtilis
2.7.2.4 42700
-
 alpha subunit, calculated from nucleotide sequence Bacillus subtilis
2.7.2.4 42700
-
 alpha subunit, calculated from nucleotide sequence Thermus thermophilus
2.7.2.4 43200
-
 alpha subunit, calculated from nucleotide sequence Thermus thermophilus
2.7.2.4 43700
-
 alpha subunit, calculated from nucleotide sequence Bacillus subtilis
2.7.2.4 43900
-
 alpha subunit, calculated from nucleotide sequence Bacillus subtilis
2.7.2.4 43900
-
 alpha subunit, calculated from nucleotide sequence Thermus thermophilus
2.7.2.4 60700
-
 alphabeta, calculated from nucleotide sequence Bacillus subtilis
2.7.2.4 60700
-
 alphabeta, calculated from nucleotide sequence Thermus thermophilus
2.7.2.4 61200
-
 alphabeta, calculated from nucleotide sequence Thermus thermophilus
2.7.2.4 62200
-
 alphabeta, calculated from nucleotide sequence Bacillus subtilis
2.7.2.4 64200
-
 alpha2, gel filtration Bacillus subtilis
2.7.2.4 64200
-
 alpha2, gel filtration Thermus thermophilus
2.7.2.4 87800
-
 alpha2, calculated from nucleotide sequence Bacillus subtilis
2.7.2.4 87800
-
 alpha2, calculated from nucleotide sequence Thermus thermophilus
2.7.2.4 90300
-
 alpha2, beta2, gel filtration Thermus thermophilus
2.7.2.4 121400
-
 alpha2, beta2, calculated from nucleotide sequence Bacillus subtilis
2.7.2.4 121400
-
 alpha2, beta2, calculated from nucleotide sequence Thermus thermophilus
2.7.2.4 122400
-
 alpha2, beta2, calculated from nucleotide sequence Thermus thermophilus
2.7.2.4 124400
-
 alpha2, beta2, calculated from nucleotide sequence Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.4 ATP + L-aspartate Bacillus subtilis
-
 ADP + phospho-L-aspartate
-
 ?
2.7.2.4 ATP + L-aspartate Thermus thermophilus
-
 ADP + phospho-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Bacillus subtilis
-
-
-
2.7.2.4 Bacillus subtilis
-
 chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus
-
2.7.2.4 Bacillus subtilis
-
 chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus, and the beta subunit from Thermus flavus
-
2.7.2.4 Thermus thermophilus
-
-
-
2.7.2.4 Thermus thermophilus
-
 chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus
-
2.7.2.4 Thermus thermophilus
-
 chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus, and the beta subunit from Thermus flavus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4
-
 Bacillus subtilis
2.7.2.4
-
 Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.4 0.38
-
 truncated protein, which contains only the N-terminal region Bacillus subtilis
2.7.2.4 5.9
-
 wild type Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
 Bacillus subtilis ADP + phospho-L-aspartate
-
 ?
2.7.2.4 ATP + L-aspartate
-
 Thermus thermophilus ADP + phospho-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.4 dimer alpha2, 2 * 43900, gel filtration, calculated from nucleotide sequence Bacillus subtilis
2.7.2.4 dimer alpha2, 2 * 43900, gel filtration, calculated from nucleotide sequence Thermus thermophilus
2.7.2.4 tetramer alpha2, beta2, 2 * 42700 + 2 * 18000, gel filtration, ultracentrifugation, calculated from nucleotide sequence Bacillus subtilis
2.7.2.4 tetramer alpha2, beta2, 2 * 42700 + 2 * 18000, gel filtration, ultracentrifugation, calculated from nucleotide sequence Thermus thermophilus
2.7.2.4 tetramer alpha2, beta2, 2 * 43200 + 2 * 18000, gel filtration, ultracentrifugation, calculated from nucleotide sequence Thermus thermophilus
2.7.2.4 tetramer alpha2, beta2, 2 * 43700 + 2 * 18500, calculated from nucleotide sequence Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.7.2.4 AK
-
 Thermus thermophilus
2.7.2.4 AK II
-
 Bacillus subtilis
2.7.2.4 aspartate kinase
-
 Bacillus subtilis
2.7.2.4 aspartate kinase
-
 Thermus thermophilus
2.7.2.4 BT
-
 Bacillus subtilis
2.7.2.4 BT
-
 Thermus thermophilus
2.7.2.4 BTT
-
 Bacillus subtilis
2.7.2.4 BTT
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.4 70
-
 AK II loses activity very rapidly with a half-life of 31.5 s Bacillus subtilis
2.7.2.4 70
-
 AK retains the full activity after 1 h of heat treatment Thermus thermophilus
2.7.2.4 70
-
 loss of activity with a half-life of 45.7 s Bacillus subtilis
2.7.2.4 70
-
 loss of activity with a half-life of 45.7 s Thermus thermophilus
2.7.2.4 70
-
 no loss of activity even after 1 h of heat treatment Bacillus subtilis
2.7.2.4 70
-
 no loss of activity even after 1 h of heat treatment Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.4 11.87
-
 ATP
-
 Bacillus subtilis
2.7.2.4 11.87
-
 ATP
-
 Thermus thermophilus
2.7.2.4 15.25
-
 ATP
-
 Bacillus subtilis
2.7.2.4 15.25
-
 ATP
-
 Thermus thermophilus
2.7.2.4 15.87
-
 ATP wild type Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.4 1.11
-
 L-threonine
-
 Bacillus subtilis
2.7.2.4 1.11
-
 L-threonine
-
 Thermus thermophilus
2.7.2.4 1.24
-
 L-threonine
-
 Thermus thermophilus
2.7.2.4 1.28
-
 L-lysine
-
 Bacillus subtilis