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Literature summary extracted from

  • Fukuda, Y.; Kihara, A.; Igarashi, Y.
    Distribution of sphingosine kinase activity in mouse tissues: contribution of SPHK1 (2003), Biochem. Biophys. Res. Commun., 309, 155-160.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.91 membrane about 30% of total activity Mus musculus 16020
-
2.7.1.91 soluble about 70% of total activity Mus musculus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.91 ATP + sphingosine Mus musculus
-
 ADP + sphingosine 1-phosphate short-living product S1P is a bioactive lipid mediator in diverse cellular processes, e.g. regulation of cell differentiation, motility, and apoptosis both extracellularly via S1P family receptors and intracellularly ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.91 Mus musculus
-
 male C57BL mice, 2 isozymes SPHK1 and SPHK2, isozyme SPHK1 exists in 2 splicing variants a and b
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.91 blood platelet
-
 Mus musculus
-
2.7.1.91 brain major isozyme is SPHK1 Mus musculus
-
2.7.1.91 colon major isozyme is SPHK1 Mus musculus
-
2.7.1.91 heart major isozyme is SPHK1 Mus musculus
-
2.7.1.91 kidney
-
 Mus musculus
-
2.7.1.91 liver
-
 Mus musculus
-
2.7.1.91 lung high activity, isozymes SPHK1 and SPHK2 Mus musculus
-
2.7.1.91 additional information isozyme tissue distribution patterns Mus musculus
-
2.7.1.91 pancreas
-
 Mus musculus
-
2.7.1.91 skeletal muscle very low activity Mus musculus
-
2.7.1.91 small intestine isozymes SPHK1 and SPHK2 Mus musculus
-
2.7.1.91 spleen isozymes SPHK1 and SPHK2 Mus musculus
-
2.7.1.91 stomach
-
 Mus musculus
-
2.7.1.91 testis
-
 Mus musculus
-
2.7.1.91 thymus high activity Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.91 0.000002
-
 skeletal muscle homogenate Mus musculus
2.7.1.91 0.00003
-
 liver homogenate Mus musculus
2.7.1.91 0.00004 0.00005 homogenates of kidney, colon, stomach, pancreas, and heart Mus musculus
2.7.1.91 0.00006 0.00007 homogenates of testis, brain, and platelet Mus musculus
2.7.1.91 0.00009
-
 homogenates of small intestine and spleen Mus musculus
2.7.1.91 0.00016
-
 thymus homogenate Mus musculus
2.7.1.91 0.00024
-
 lung homogenate Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.91 ATP + sphingosine
-
 Mus musculus ADP + sphingosine 1-phosphate short-living product S1P is a bioactive lipid mediator in diverse cellular processes, e.g. regulation of cell differentiation, motility, and apoptosis both extracellularly via S1P family receptors and intracellularly ?
2.7.1.91 ATP + sphingosine isozymes SPHK1 is about 100fold more active than isozyme SPHK2 Mus musculus ADP + sphingosine 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.91 sphinganine kinase
-
 Mus musculus
2.7.1.91 SPHK
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.91 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.91 7.5
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.91 ATP
-
 Mus musculus