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Literature summary extracted from

  • Gao, Y.; Kawano, K.; Yoshiyama, S.; Kawamichi, H.; Wang, X.; Nakamura, A.; Kohama, K.
    Myosin light chain kinase stimulates smooth muscle myosin ATPase activity by binding to the myosin heads without phosphorylating the myosin light chain (2003), Biochem. Biophys. Res. Commun., 305, 16-21.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.18 expression of a C-terminal enzyme fragment comprising residues 860-1176 as GST-fusion protein in Escherichia coli strain Bl21(DE3), the GST-tag slightly reduces the activity of the recombinant enzyme fragment compared to the untagged fragment Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+ complex formation with calmodulin Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + [myosin light chain] Bos taurus the multifunctional regulatory enzyme stimulates smooth muscle myosin ATPase activity by binding to the myosin heads without phosphorylating the myosin light chain, kinetics, overview ADP + [myosin light chain] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.18 recombinant GST-tagged C-terminal enzyme fragment by glutathione affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 smooth muscle
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + [myosin light chain]
-
 Bos taurus ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 ATP + [myosin light chain] the multifunctional regulatory enzyme stimulates smooth muscle myosin ATPase activity by binding to the myosin heads without phosphorylating the myosin light chain, kinetics, overview Bos taurus ADP + [myosin light chain] phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 MLCK
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 ATP
-
 Bos taurus
2.7.11.18 Calmodulin binding site I at residue 1002-1022, binding site II at residues 26-42, complex formation with Ca2+ Bos taurus