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Literature summary extracted from

  • Krawiec, K.; Kierdaszuk, B.; Shugar, D.
    Inorganic tripolyphosphate (PPP(i)) as a phosphate donor for human deoxyribonucleoside kinases (2003), Biochem. Biophys. Res. Commun., 301, 192-197.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.113 ATP stabilizes the enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.76 diphosphate competitive inhibition Homo sapiens
2.7.1.113 diphosphate competitive inhibition Homo sapiens
2.7.1.145 diphosphate competitive versus ATP Drosophila melanogaster
2.7.1.145 Triphosphate
-
 Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.76 additional information
-
 additional information the reaction with triphosphate follows strict Michaelis-Menten kinetics, while ATP exhibits negative cooperativity Homo sapiens
2.7.1.76 0.007
-
 ATP pH 7.5, 37°C, with deoxycytidine Homo sapiens
2.7.1.76 0.06
-
 ATP pH 7.5, 37°C, with deoxyadenosine Homo sapiens
2.7.1.76 0.074
-
 Triphosphate pH 7.5, 37°C, with deoxycytidine Homo sapiens
2.7.1.113 additional information
-
 additional information the reaction with triphosphate follows strict Michaelis-Menten kinetics, while ATP exhibits negative cooperativity Homo sapiens
2.7.1.113 0.02
-
 ATP pH 7.5, 37°C Homo sapiens
2.7.1.113 0.092
-
 Triphosphate pH 7.5, 37°C Homo sapiens
2.7.1.145 0.0014
-
 ATP
-
 Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.76 Mg2+
-
 Homo sapiens
2.7.1.113 Mg2+
-
 Homo sapiens
2.7.1.145 Mg2+
-
 Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.76 ATP + 2'-deoxyadenosine Homo sapiens
-
 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ir
2.7.1.76 ATP + 2'-deoxycytidine Homo sapiens
-
 ADP + 2'-deoxycytidine 5'-phosphate
-
 ir
2.7.1.113 ATP + 2'-deoxyguanosine Homo sapiens
-
 ADP + 2'-deoxyguanosine 5'-phosphate
-
 ir
2.7.1.145 ATP + 2'-deoxynucleoside Drosophila melanogaster
-
 ADP + 2'-deoxynucleoside 5'-phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.76 Homo sapiens
-
-
-
2.7.1.113 Homo sapiens
-
-
-
2.7.1.145 Drosophila melanogaster
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.76 0.007
-
 purified recombinant enzyme, substrates deoxycytidine and ATP Homo sapiens
2.7.1.76 0.43
-
 purified recombinant enzyme, substrates deoxyadenosine and ATP Homo sapiens
2.7.1.113 0.007
-
 purified recombinant enzyme, substrates deoxyguanosine and ATP Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.76 ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ir
2.7.1.76 ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ir
2.7.1.76 additional information no activity with diphosphate as phosphate donor, no activity with triphosphate and 2'-deoxyadenosine as substrates Homo sapiens ?
-
 ?
2.7.1.76 triphosphate + 2'-deoxycytidine
-
 Homo sapiens diphosphate + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.113 ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ir
2.7.1.113 additional information no activity with diphosphate as phosphate donor Homo sapiens ?
-
 ?
2.7.1.113 triphosphate + 2'-deoxyguanosine triphosphate is active with 2'deoxyguanosine, 50% of the activity with ATP Homo sapiens diphosphate + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyadenosine
-
 Drosophila melanogaster ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine
-
 Drosophila melanogaster ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxynucleoside
-
 Drosophila melanogaster ADP + 2'-deoxynucleoside 5'-phosphate
-
 ir
2.7.1.145 additional information no activity with diphosphate, or triphosphate as phosphate donors Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.76 dCK
-
 Homo sapiens
2.7.1.113 DGK
-
 Homo sapiens
2.7.1.145 dNK
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.76 37
-
 assay at Homo sapiens
2.7.1.113 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.76 7.5
-
 assay at Homo sapiens
2.7.1.113 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.76 ATP
-
 Homo sapiens
2.7.1.76 additional information triphosphate is active with 2'-deoxycytidine, 40% of the activity with ATP, not with 2'-deoxyadenosine, no activity with diphosphate as phosphate donor Homo sapiens
2.7.1.113 ATP preferred phosphate donor substrate, stabilizes the enzyme Homo sapiens
2.7.1.113 additional information no activity with diphosphate as phosphate donor Homo sapiens
2.7.1.145 ATP cannot be substituted by with diphosphate, triphosphate, or polyphosphate as phosphate donors Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.76 0.08
-
 diphosphate pH 7.5, 37°C, with deoxyadenosine, versus ATP Homo sapiens
2.7.1.76 0.31
-
 diphosphate pH 7.5, 37°C, with deoxycytidine, versus ATP or triphosphate Homo sapiens
2.7.1.145 0.08
-
 diphosphate versus ATP, with substrate 2'-deoxycytidine Drosophila melanogaster
2.7.1.145 0.143
-
 Triphosphate versus ATP, with substrate 2'-deoxycytidine Drosophila melanogaster