BRENDA - Enzyme Database show

Inorganic tripolyphosphate (PPP(i)) as a phosphate donor for human deoxyribonucleoside kinases

Krawiec, K.; Kierdaszuk, B.; Shugar, D.; Biochem. Biophys. Res. Commun. 301, 192-197 (2003)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.1.113
ATP stabilizes the enzyme
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.76
diphosphate
competitive inhibition
Homo sapiens
2.7.1.113
diphosphate
competitive inhibition
Homo sapiens
2.7.1.145
diphosphate
competitive versus ATP
Drosophila melanogaster
2.7.1.145
Triphosphate
-
Drosophila melanogaster
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.76
additional information
-
additional information
the reaction with triphosphate follows strict Michaelis-Menten kinetics, while ATP exhibits negative cooperativity
Homo sapiens
2.7.1.76
0.007
-
ATP
pH 7.5, 37°C, with deoxycytidine
Homo sapiens
2.7.1.76
0.06
-
ATP
pH 7.5, 37°C, with deoxyadenosine
Homo sapiens
2.7.1.76
0.074
-
Triphosphate
pH 7.5, 37°C, with deoxycytidine
Homo sapiens
2.7.1.113
additional information
-
additional information
the reaction with triphosphate follows strict Michaelis-Menten kinetics, while ATP exhibits negative cooperativity
Homo sapiens
2.7.1.113
0.02
-
ATP
pH 7.5, 37°C
Homo sapiens
2.7.1.113
0.092
-
Triphosphate
pH 7.5, 37°C
Homo sapiens
2.7.1.145
0.0014
-
ATP
-
Drosophila melanogaster
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.76
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
2.7.1.145
Mg2+
-
Drosophila melanogaster
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.76
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
ir
2.7.1.76
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
ir
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
ir
2.7.1.145
ATP + 2'-deoxynucleoside
Drosophila melanogaster
-
ADP + 2'-deoxynucleoside 5'-phosphate
-
-
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.76
Homo sapiens
-
-
-
2.7.1.113
Homo sapiens
-
-
-
2.7.1.145
Drosophila melanogaster
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.76
0.007
-
purified recombinant enzyme, substrates deoxycytidine and ATP
Homo sapiens
2.7.1.76
0.43
-
purified recombinant enzyme, substrates deoxyadenosine and ATP
Homo sapiens
2.7.1.113
0.007
-
purified recombinant enzyme, substrates deoxyguanosine and ATP
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + 2'-deoxyadenosine
-
660855
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
ir
2.7.1.76
ATP + 2'-deoxycytidine
-
660855
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
ir
2.7.1.76
additional information
no activity with diphosphate as phosphate donor, no activity with triphosphate and 2'-deoxyadenosine as substrates
660855
Homo sapiens
?
-
-
-
-
2.7.1.76
triphosphate + 2'-deoxycytidine
-
660855
Homo sapiens
diphosphate + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
660855
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
ir
2.7.1.113
additional information
no activity with diphosphate as phosphate donor
660855
Homo sapiens
?
-
-
-
-
2.7.1.113
triphosphate + 2'-deoxyguanosine
triphosphate is active with 2'deoxyguanosine, 50% of the activity with ATP
660855
Homo sapiens
diphosphate + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyadenosine
-
660855
Drosophila melanogaster
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
-
660855
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxynucleoside
-
660855
Drosophila melanogaster
ADP + 2'-deoxynucleoside 5'-phosphate
-
-
-
ir
2.7.1.145
additional information
no activity with diphosphate, or triphosphate as phosphate donors
660855
Drosophila melanogaster
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.76
37
-
assay at
Homo sapiens
2.7.1.113
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.76
7.5
-
assay at
Homo sapiens
2.7.1.113
7.5
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.76
ATP
-
Homo sapiens
2.7.1.76
additional information
triphosphate is active with 2'-deoxycytidine, 40% of the activity with ATP, not with 2'-deoxyadenosine, no activity with diphosphate as phosphate donor
Homo sapiens
2.7.1.113
ATP
preferred phosphate donor substrate, stabilizes the enzyme
Homo sapiens
2.7.1.113
additional information
no activity with diphosphate as phosphate donor
Homo sapiens
2.7.1.145
ATP
cannot be substituted by with diphosphate, triphosphate, or polyphosphate as phosphate donors
Drosophila melanogaster
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.76
0.08
-
diphosphate
pH 7.5, 37°C, with deoxyadenosine, versus ATP
Homo sapiens
2.7.1.76
0.31
-
diphosphate
pH 7.5, 37°C, with deoxycytidine, versus ATP or triphosphate
Homo sapiens
2.7.1.145
0.08
-
diphosphate
versus ATP, with substrate 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
0.143
-
Triphosphate
versus ATP, with substrate 2'-deoxycytidine
Drosophila melanogaster
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.76
ATP
-
Homo sapiens
2.7.1.76
additional information
triphosphate is active with 2'-deoxycytidine, 40% of the activity with ATP, not with 2'-deoxyadenosine, no activity with diphosphate as phosphate donor
Homo sapiens
2.7.1.113
ATP
preferred phosphate donor substrate, stabilizes the enzyme
Homo sapiens
2.7.1.113
additional information
no activity with diphosphate as phosphate donor
Homo sapiens
2.7.1.145
ATP
cannot be substituted by with diphosphate, triphosphate, or polyphosphate as phosphate donors
Drosophila melanogaster
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.113
ATP stabilizes the enzyme
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.76
diphosphate
competitive inhibition
Homo sapiens
2.7.1.113
diphosphate
competitive inhibition
Homo sapiens
2.7.1.145
diphosphate
competitive versus ATP
Drosophila melanogaster
2.7.1.145
Triphosphate
-
Drosophila melanogaster
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.76
0.08
-
diphosphate
pH 7.5, 37°C, with deoxyadenosine, versus ATP
Homo sapiens
2.7.1.76
0.31
-
diphosphate
pH 7.5, 37°C, with deoxycytidine, versus ATP or triphosphate
Homo sapiens
2.7.1.145
0.08
-
diphosphate
versus ATP, with substrate 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
0.143
-
Triphosphate
versus ATP, with substrate 2'-deoxycytidine
Drosophila melanogaster
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.76
additional information
-
additional information
the reaction with triphosphate follows strict Michaelis-Menten kinetics, while ATP exhibits negative cooperativity
Homo sapiens
2.7.1.76
0.007
-
ATP
pH 7.5, 37°C, with deoxycytidine
Homo sapiens
2.7.1.76
0.06
-
ATP
pH 7.5, 37°C, with deoxyadenosine
Homo sapiens
2.7.1.76
0.074
-
Triphosphate
pH 7.5, 37°C, with deoxycytidine
Homo sapiens
2.7.1.113
additional information
-
additional information
the reaction with triphosphate follows strict Michaelis-Menten kinetics, while ATP exhibits negative cooperativity
Homo sapiens
2.7.1.113
0.02
-
ATP
pH 7.5, 37°C
Homo sapiens
2.7.1.113
0.092
-
Triphosphate
pH 7.5, 37°C
Homo sapiens
2.7.1.145
0.0014
-
ATP
-
Drosophila melanogaster
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.76
Mg2+
-
Homo sapiens
2.7.1.113
Mg2+
-
Homo sapiens
2.7.1.145
Mg2+
-
Drosophila melanogaster
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.76
ATP + 2'-deoxyadenosine
Homo sapiens
-
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
ir
2.7.1.76
ATP + 2'-deoxycytidine
Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
-
ir
2.7.1.113
ATP + 2'-deoxyguanosine
Homo sapiens
-
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
ir
2.7.1.145
ATP + 2'-deoxynucleoside
Drosophila melanogaster
-
ADP + 2'-deoxynucleoside 5'-phosphate
-
-
ir
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.76
0.007
-
purified recombinant enzyme, substrates deoxycytidine and ATP
Homo sapiens
2.7.1.76
0.43
-
purified recombinant enzyme, substrates deoxyadenosine and ATP
Homo sapiens
2.7.1.113
0.007
-
purified recombinant enzyme, substrates deoxyguanosine and ATP
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + 2'-deoxyadenosine
-
660855
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
ir
2.7.1.76
ATP + 2'-deoxycytidine
-
660855
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
ir
2.7.1.76
additional information
no activity with diphosphate as phosphate donor, no activity with triphosphate and 2'-deoxyadenosine as substrates
660855
Homo sapiens
?
-
-
-
-
2.7.1.76
triphosphate + 2'-deoxycytidine
-
660855
Homo sapiens
diphosphate + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.113
ATP + 2'-deoxyguanosine
-
660855
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
ir
2.7.1.113
additional information
no activity with diphosphate as phosphate donor
660855
Homo sapiens
?
-
-
-
-
2.7.1.113
triphosphate + 2'-deoxyguanosine
triphosphate is active with 2'deoxyguanosine, 50% of the activity with ATP
660855
Homo sapiens
diphosphate + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyadenosine
-
660855
Drosophila melanogaster
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxycytidine
-
660855
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxynucleoside
-
660855
Drosophila melanogaster
ADP + 2'-deoxynucleoside 5'-phosphate
-
-
-
ir
2.7.1.145
additional information
no activity with diphosphate, or triphosphate as phosphate donors
660855
Drosophila melanogaster
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.76
37
-
assay at
Homo sapiens
2.7.1.113
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.76
7.5
-
assay at
Homo sapiens
2.7.1.113
7.5
-
assay at
Homo sapiens