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Literature summary extracted from

  • Etienne, F.; Spector, D.; Brot, N.; Weissbach, H.
    A methionine sulfoxide reductase in Escherichia coli that reduces the R enantiomer of methionine sulfoxide (2003), Biochem. Biophys. Res. Commun., 300, 378-382.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.14 Escherichia coli
-
-
-
1.8.4.14 Escherichia coli MC1061
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.14 partial Escherichia coli
1.8.4.14 partially purified by (NH4)2SO4 precipitation (30-60% saturation) from MsrA/MsrB double mutants Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.14 L-methionine (R)-sulfoxide + thioredoxin
-
 Escherichia coli L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.14 L-methionine (R)-sulfoxide + thioredoxin
-
 Escherichia coli MC1061 L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.14 L-methionine + thioredoxin disulfide + H2O enzyme specific for free L-methionine-(R)-sulfoxide and not met-R-(o) in peptide linkage Escherichia coli L-methionine (R)-S-oxide + thioredoxin
-
 ?
1.8.4.14 L-methionine + thioredoxin disulfide + H2O enzyme specific for free L-methionine-(R)-sulfoxide and not met-R-(o) in peptide linkage Escherichia coli MC1061 L-methionine (R)-S-oxide + thioredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.14 fRMsr
-
 Escherichia coli
1.8.4.14 methionine sulfoxide reductase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.14 dithiothreitol DTT can replace NADPH, but is much less effective Escherichia coli
1.8.4.14 NADPH absolute requirement Escherichia coli
1.8.4.14 thioredoxin omission results in a 70% drop in activity Escherichia coli