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Literature summary extracted from

  • Soderberg, T.; Alver, R.C.
    Transaldolase of Methanocaldococcus jannaschii (2004), Archaea, 1, 255-262.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.2 overexpression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.2 D-ribose-5-phosphate above 15 mM Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.2 0.0156
-
 D-erythrose 4-phosphate pH 7.5, 25°C Methanocaldococcus jannaschii
2.2.1.2 0.0278
-
 D-erythrose 4-phosphate pH 7.5, 50°C Methanocaldococcus jannaschii
2.2.1.2 0.64
-
 D-fructose 6-phosphate pH 7.5, 25°C Methanocaldococcus jannaschii
2.2.1.2 0.65
-
 D-fructose 6-phosphate pH 7.5, 50°C Methanocaldococcus jannaschii
2.2.1.2 2.2
-
 D-ribose 5-phosphate pH 7.5, 50°C, concentration of ribose-5-phosphate between 0.5 and 15 mM Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.2 23960
-
 10 * 24000, SDS-PAGE, 10 * 23960, calculated Methanocaldococcus jannaschii
2.2.1.2 24000
-
 10 * 24000, SDS-PAGE, 10 * 23960, calculated Methanocaldococcus jannaschii
2.2.1.2 271000
-
 gel filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.2 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.2 recombinant enzyme Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.2 1.1
-
 pH 7.5, 25°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.2 D-fructose 6-phosphate + D-erythrose 4-phosphate
-
 Methanocaldococcus jannaschii ?
-
 ?
2.2.1.2 D-fructose 6-phosphate + D-ribose 5-phosphate
-
 Methanocaldococcus jannaschii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.2 decamer 10 * 24000, SDS-PAGE, 10 * 23960, calculated Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.2 25
-
 3 weeks, no loss of activity Methanocaldococcus jannaschii
2.2.1.2 80
-
 4 h, no loss of activity, 24 h, 50% residual activity Methanocaldococcus jannaschii
2.2.1.2 90
-
 15 min, 20% loss of activity Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.2.1.2 7 8.5
-
 Methanocaldococcus jannaschii