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Literature summary extracted from

  • Reshetnikov, A.S.; Khmelenina, V.N.; Trotsenko, Y.A.
    Characterization of the ectoine biosynthesis genes of haloalkalotolerant obligate methanotroph "Methylomicrobium alcaliphilum 20Z" (2006), Arch. Microbiol., 184, 286-297.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.108 additional information enzyme expression is 12fold induced by NaCl at 1 M Methylotuvimicrobium alcaliphilum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.178 expression in Escherichia coli Methylotuvimicrobium alcaliphilum
2.6.1.46 expressed in Escherichia coli Methylotuvimicrobium alcaliphilum
4.2.1.108 gene ectC located in the singel operon ectABCask, DNA sequence determination and analysis, genetic organization, expression in Escherichia coli strain X1-Blue Methylotuvimicrobium alcaliphilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.108 NaCl required for activity Methylotuvimicrobium alcaliphilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate Methylotuvimicrobium alcaliphilum enzyme is involved in ectoine biosynthesis pathway CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate Methylotuvimicrobium alcaliphilum 20Z enzyme is involved in ectoine biosynthesis pathway CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate Methylotuvimicrobium alcaliphilum final step in L-ectoine biosynthesis, pathway overview L-ectoine + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.178 Methylotuvimicrobium alcaliphilum
-
-
-
2.3.1.178 Methylotuvimicrobium alcaliphilum 20Z
-
-
-
2.6.1.46 Methylotuvimicrobium alcaliphilum
-
-
-
2.6.1.46 Methylotuvimicrobium alcaliphilum 20Z
-
-
-
4.2.1.108 Methylotuvimicrobium alcaliphilum Q4JQJ3 ectABCask gene cluster sequence; 20Z, obligate methanotroph, gene ectC
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.108 additional information cells are grown in an atmosphere of air-methane 1:1 in 1 M NaCl Methylotuvimicrobium alcaliphilum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate enzyme is involved in ectoine biosynthesis pathway Methylotuvimicrobium alcaliphilum CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate enzyme is involved in ectoine biosynthesis pathway Methylotuvimicrobium alcaliphilum 20Z CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.6.1.46 L-2,4-diaminobutanoate + 2-oxoglutarate
-
 Methylotuvimicrobium alcaliphilum L-aspartate 4-semialdehyde + L-glutamate
-
 ?
2.6.1.46 L-2,4-diaminobutanoate + 2-oxoglutarate
-
 Methylotuvimicrobium alcaliphilum 20Z L-aspartate 4-semialdehyde + L-glutamate
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, pathway overview Methylotuvimicrobium alcaliphilum L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid Methylotuvimicrobium alcaliphilum L-ectoine + H2O NMR product identification r

Synonyms

EC Number Synonyms Comment Organism
2.3.1.178 2,4-diaminobutanoate acetyltransferase
-
 Methylotuvimicrobium alcaliphilum
2.3.1.178 DAB acetyltransferase
-
 Methylotuvimicrobium alcaliphilum
2.3.1.178 DABA acetyltransferase
-
 Methylotuvimicrobium alcaliphilum
2.3.1.178 DABAcT
-
 Methylotuvimicrobium alcaliphilum
2.3.1.178 diaminobutyric acid acetyltransferase
-
 Methylotuvimicrobium alcaliphilum
2.3.1.178 EctA
-
 Methylotuvimicrobium alcaliphilum
2.3.1.178 L-2,4-diaminobutanoate acetyltransferase
-
 Methylotuvimicrobium alcaliphilum
2.3.1.178 L-2,4-diaminobutyrate acetyltransferase
-
 Methylotuvimicrobium alcaliphilum
2.6.1.46 DAB transaminase
-
 Methylotuvimicrobium alcaliphilum
2.6.1.46 diaminobutyric acid transaminase
-
 Methylotuvimicrobium alcaliphilum
2.6.1.46 EctB
-
 Methylotuvimicrobium alcaliphilum
2.6.1.46 L-2,4-diaminobutyric acid aminotransferase
-
 Methylotuvimicrobium alcaliphilum
4.2.1.108 EctC
-
 Methylotuvimicrobium alcaliphilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.108 20
-
 assay at Methylotuvimicrobium alcaliphilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.108 8.5
-
 assay at Methylotuvimicrobium alcaliphilum