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Literature summary extracted from

  • Huergo, L.F.; Souza, E.M.; Steffens, M.B.; Yates, M.G.; Pedrosa, F.O.; Chubatsu, L.S.
    Effects of over-expression of the regulatory enzymes DraT and DraG on the ammonium-dependent post-translational regulation of nitrogenase reductase in Azospirillum brasilense (2005), Arch. Microbiol., 183, 209-217.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.37 functional overexpression in Escherichia coli as N-terminally His6-tagged protein, leading to NifH Fe-Protein modification in absence of ammonium, i.e. the recombinant His-tagged enzyme loses its regulation Azospirillum brasilense

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.24 additional information overexpression of enzyme results in only partial ADP-ribosylation of Fe protein by adding ammonium. Loss of regulation after overespression may be due to titrating out negative regulators Azospirillum brasilense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.37 NAD+ + [dinitrogen reductase] Azospirillum brasilense enzyme is involved in regulation of nitrogen fixation, enzyme acts together with the dinitrogenase reductase glycohydrolase DraG in posttranslational modification of the nitrogenase involving NH4Cl, overview, regulation of the enzyme, overview nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.37 Azospirillum brasilense
-
-
-
3.2.2.24 Azospirillum brasilense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.37 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Azospirillum brasilense

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.37 additional information
-
-
 Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.37 NAD+ + [dinitrogen reductase]
-
 Azospirillum brasilense nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
 r
2.4.2.37 NAD+ + [dinitrogen reductase] enzyme is involved in regulation of nitrogen fixation, enzyme acts together with the dinitrogenase reductase glycohydrolase DraG in posttranslational modification of the nitrogenase involving NH4Cl, overview, regulation of the enzyme, overview Azospirillum brasilense nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.2.37 dinitrogenase reductase ADP-ribosyltransferase
-
 Azospirillum brasilense
2.4.2.37 DRAT
-
 Azospirillum brasilense