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Literature summary extracted from

  • Labes, A.; Schonheit, P.
    ADP-dependent glucokinase from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324 (2003), Arch. Microbiol., 180, 69-75.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.147 0.07
-
 ADP pH 7.6, 50°C Archaeoglobus fulgidus
2.7.1.147 0.78
-
 D-glucose pH 7.6, 50°C Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.147 Ca2+
-
 Archaeoglobus fulgidus
2.7.1.147 Co2+
-
 Archaeoglobus fulgidus
2.7.1.147 Cu2+
-
 Archaeoglobus fulgidus
2.7.1.147 Mg2+ at 4 mM, optimal ratio of ADP:MgCl2 is 1:2 Archaeoglobus fulgidus
2.7.1.147 Mn2+
-
 Archaeoglobus fulgidus
2.7.1.147 additional information divalent cations are required, Mg2+, Mn2+, and Ca2+ are most effective, Cu2+, Zn2+, Ni2+, and Cu2+ can partially substitute Archaeoglobus fulgidus
2.7.1.147 Ni2+
-
 Archaeoglobus fulgidus
2.7.1.147 Zn2+
-
 Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.147 50000
-
 gel filtration Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.147 ADP + D-glucose Archaeoglobus fulgidus first step of the Embden-Meyerhoff pathway AMP + D-glucose 6-phosphate
-
 r
2.7.1.147 ADP + D-glucose Archaeoglobus fulgidus 7324 first step of the Embden-Meyerhoff pathway AMP + D-glucose 6-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.147 Archaeoglobus fulgidus
-
-
-
2.7.1.147 Archaeoglobus fulgidus 7324
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.7.1.147 enzyme is not sensitive to oxygen Archaeoglobus fulgidus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.147 600fold to homogeneity by ultracentrifugation, ion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, hydroxyapatite chromatography, and 2 additional ion exchange chromatographic steps Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.147 additional information
-
-
 Archaeoglobus fulgidus
2.7.1.147 58.9
-
 purified enzyme Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.147 ADP + D-glucosamine the enzyme is highly specific for both substrates Archaeoglobus fulgidus AMP + D-glucosamine 6-phosphate
-
 r
2.7.1.147 ADP + D-glucosamine the enzyme is highly specific for both substrates Archaeoglobus fulgidus 7324 AMP + D-glucosamine 6-phosphate
-
 r
2.7.1.147 ADP + D-glucose first step of the Embden-Meyerhoff pathway Archaeoglobus fulgidus AMP + D-glucose 6-phosphate
-
 r
2.7.1.147 ADP + D-glucose the enzyme is highly specific for both substrates Archaeoglobus fulgidus AMP + D-glucose 6-phosphate
-
 r
2.7.1.147 ADP + D-glucose first step of the Embden-Meyerhoff pathway Archaeoglobus fulgidus 7324 AMP + D-glucose 6-phosphate
-
 r
2.7.1.147 ADP + D-glucose the enzyme is highly specific for both substrates Archaeoglobus fulgidus 7324 AMP + D-glucose 6-phosphate
-
 r
2.7.1.147 additional information 2-deoxyglucose is a poor substrate, no activity with ATP, CDP, UDP, or GDP as phosphate donors, no activity with mannose, fructose, and fructose 6-phosphate as phosphate acceptors Archaeoglobus fulgidus ?
-
 ?
2.7.1.147 additional information 2-deoxyglucose is a poor substrate, no activity with ATP, CDP, UDP, or GDP as phosphate donors, no activity with mannose, fructose, and fructose 6-phosphate as phosphate acceptors Archaeoglobus fulgidus 7324 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.147 monomer 1 * 50000, SDS-PAGE Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.147 ADP-dependent glucokinase
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.147 83
-
-
 Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.147 80
-
 stable up to Archaeoglobus fulgidus
2.7.1.147 100
-
 in absence of salt loss of over 80% activity within 30 min, in presence of 1 M ammonium sulfate 70% reaminin g activity after 120 min, in presence of 1 M NaCl 40% remaining activity after 120 min Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.147 7.6
-
-
 Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.147 ADP at 2 mM, optimal ratio of ADP:MgCl2 is 1:2 Archaeoglobus fulgidus