Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Aboulmagd, E.; Oppermann-Sanio, F.B.; Steinbüchel, A.
    Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308 (2000), Arch. Microbiol., 174, 297-306.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.29 expression in Escherichia coli TOP10 cells Geminocystis herdmanii PCC 6308
6.3.2.30 expression in Escherichia coli TOP10 cells Synechocystis sp. PCC 6803

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.29 Synechocystis sp.
-
-
-
6.3.2.29 Synechocystis sp. PCC 6804
-
-
-
6.3.2.30 Synechocystis sp. PCC 6805
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.29 from Synechocystis and from recombinant Escherichia coli cells Geminocystis herdmanii PCC 6308
6.3.2.30 from Synechocystis and from recombinant Escherichia coli cells Synechocystis sp. PCC 6803

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp = ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp i.e. the second reaction of cyanophycin synthesis catalysed by cyanophycin synthase, the first reaction is catalysed by the enzyme's other active centre Geminocystis herdmanii PCC 6308
a
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 i.e. the first reaction of cyanophycin synthesis, the second reaction is catalysed by the enzyme's other active centre Synechocystis sp. PCC 6803
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.29 0.0091
-
 enzyme isolated from Synechocystis cells, 28°C Geminocystis herdmanii PCC 6308
6.3.2.29 0.027
-
 recombinant enzyme isolated from Escherichia coli Geminocystis herdmanii PCC 6308

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp [L-Asp(4-L-Arg)]n-Asp is a cyanophycin molecule with a C-terminal L-Asp residue that is not linked to an L-Arg residue via its beta-carboxy group, this intermediate is produced in the first reaction catalysed by cyanophycin synthase Geminocystis herdmanii PCC 6308 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp no formation of AMP ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg a small amount of cyanophycin is required as a primer Synechocystis sp. PCC 6803 ADP + phosphate + [L-Asp(4-L-Arg)]n+1 no formation of AMP, [L-Asp(3-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.29 CphA
-
 Geminocystis herdmanii PCC 6308
6.3.2.29 cyanophycin synthetase
-
 Geminocystis herdmanii PCC 6308
6.3.2.30 CphA
-
 Synechocystis sp. PCC 6803
6.3.2.30 cyanophycin synthetase
-
 Synechocystis sp. PCC 6803