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Literature summary extracted from

  • Walkup, A.S.; Appling, D.R.
    Enzymatic characterization of human mitochondrial C1-tetrahydrofolate synthase (2005), Arch. Biochem. Biophys., 442, 196-205.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.3 expression of the mitochondrial isozyme as maltose-binding protein fusion protein in Escherichia coli, overexpression in yeast Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.3 additional information
-
additional information steady-state kinetics, recombinant enzyme Homo sapiens
6.3.4.3 0.0036
-
(6R,S)-tetrahydrofolate pteroylpentaglutamate recombinant enzyme, 30°C Homo sapiens
6.3.4.3 0.016
-
(6R,S)-tetrahydrofolate pteroyltriglutamate recombinant enzyme, 30°C Homo sapiens
6.3.4.3 0.04
-
ATP recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate pteroyltriglutamate or (6R,S)-tetrahydrofolate pteroylpentaglutamate and formate Homo sapiens
6.3.4.3 0.15
-
formate recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate pteroylpentaglutamate Homo sapiens
6.3.4.3 0.2
-
ATP above, recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate monoglutamate and formate Homo sapiens
6.3.4.3 0.44
-
formate recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate pteroyltriglutamate Homo sapiens
6.3.4.3 0.5
-
(6R,S)-tetrahydrofolate monoglutamate above, recombinant enzyme, 30°C Homo sapiens
6.3.4.3 11.8
-
formate recombinant enzyme, 30°C, with substrate (6R,S)-tetrahydrofolate monoglutamate Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.3 mitochondrion mitochondrial isozyme Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.3 K+ activates at 100-200 mM, inhibitory above 200 mM Homo sapiens
6.3.4.3 Mg2+
-
Homo sapiens
6.3.4.3 NH4+ activates, maximal at 100 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.3 102000
-
2 * 102000, homodimer, sequence calculation Homo sapiens
6.3.4.3 143000
-
recombinant mitochondrial isozyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.3 additional information Homo sapiens enzyme is involved in the folate-mediated one-carbon metabolism, overview ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.3 recombinant maltose-binding protein fusion mitochondrial isozyme from Escherichia coli by metal affinity chromatography and gel filtration, the MBP-tag is cleaved off by TEV protease Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.3 ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate the enzyme is not trifunctional showing only 10-formyl-THS synthetase activity, enzyme evolutionary considerations Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.3 ATP + (6R,S)-tetrahydrofolate pteroylpentaglutamate
-
Homo sapiens ADP + phosphate + 10-formyltetrahydrofolate pteroylpentaglutamate
-
?
6.3.4.3 ATP + (6R,S)-tetrahydrofolate pteroyltriglutamate
-
Homo sapiens ADP + phosphate + 10-formyltetrahydrofolate pteroyltriglutamate
-
?
6.3.4.3 ATP + formate + (6R,S)-tetrahydrofolate monoglutamate
-
Homo sapiens ADP + phosphate + 10-formyltetrahydrofolate monoglutamate
-
?
6.3.4.3 additional information enzyme is involved in the folate-mediated one-carbon metabolism, overview Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
6.3.4.3 dimer 2 * 102000, homodimer, sequence calculation Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.3.4.3 10-formyl-THF synthetase
-
Homo sapiens
6.3.4.3 C1-tetrahydrofolate synthase
-
Homo sapiens
6.3.4.3 C1-THF synthase
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.3 30
-
assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.3 2.6
-
(6R,S)-tetrahydrofolate pteroylpentaglutamate recombinant enzyme, 30°C Homo sapiens
6.3.4.3 10
-
(6R,S)-tetrahydrofolate pteroyltriglutamate recombinant enzyme, 30°C Homo sapiens
6.3.4.3 16
-
(6R,S)-tetrahydrofolate monoglutamate above, recombinant enzyme, 30°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.3 ATP
-
Homo sapiens