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Literature summary extracted from

  • Raman, J.; Sumathy, K.; Anand, R.P.; Balaram, H.
    A non-active site mutation in human hypoxanthine guanine phosphoribosyltransferase expands substrate specificity (2004), Arch. Biochem. Biophys., 427, 116-122.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.8 expression of wild-type and mutant enzyme in Escherichia coli, functional complementation study Plasmodium falciparum
2.4.2.8 expression of wild-type and mutant enzymes in Escherichia coli, functional complementation study Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.8 F36A site-directed mutagenesis, unaltered substrate specificity compared to the wild-type enzyme Homo sapiens
2.4.2.8 F36E site-directed mutagenesis, unaltered substrate specificity compared to the wild-type enzyme Homo sapiens
2.4.2.8 F36G site-directed mutagenesis, inactive mutant Homo sapiens
2.4.2.8 F36K site-directed mutagenesis, unaltered substrate specificity compared to the wild-type enzyme Homo sapiens
2.4.2.8 F36L random mutagenesis, mutant phosphoribosylates xanthine, mutant does not bind the purine substrate directly, long-range modulation via loop IV influence the substrate specificity, altered enzyme stability Homo sapiens
2.4.2.8 F36W site-directed mutagenesis, unaltered substrate specificity compared to the wild-type enzyme Homo sapiens
2.4.2.8 L44F site-directed mutagenesis, mutant does not phosphoribosylate guanine and xanthine, modulation via loop IV influence the substrate specificity, altered enzyme stability Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.001
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant mutant F36L, with xanthine Homo sapiens
2.4.2.8 0.001
-
 guanine pH 7.4, 22°C, recombinant wild-type enzyme Plasmodium falciparum
2.4.2.8 0.001
-
 hypoxanthine pH 7.4, 22°C, recombinant wild-type enzyme and mutant L44F Plasmodium falciparum
2.4.2.8 0.0014
-
 hypoxanthine pH 7.4, 22°C, recombinant wild-type enzyme Homo sapiens
2.4.2.8 0.0016
-
 hypoxanthine pH 7.4, 22°C, recombinant mutant F36K Homo sapiens
2.4.2.8 0.0018
-
 hypoxanthine pH 7.4, 22°C, recombinant mutant F36G Homo sapiens
2.4.2.8 0.002
-
 guanine pH 7.4, 22°C, recombinant mutant F36L Homo sapiens
2.4.2.8 0.002
-
 guanine pH 7.4, 22°C, recombinant mutant L44F Plasmodium falciparum
2.4.2.8 0.0029
-
 guanine pH 7.4, 22°C, recombinant mutant F36K Homo sapiens
2.4.2.8 0.0044
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant mutant F36K, with hypoxanthine Homo sapiens
2.4.2.8 0.0044
-
 hypoxanthine pH 7.4, 22°C, recombinant mutant F36L Homo sapiens
2.4.2.8 0.0045
-
 guanine pH 7.4, 22°C, recombinant wild-type enzyme Homo sapiens
2.4.2.8 0.0061
-
 guanine pH 7.4, 22°C, recombinant mutant F36G Homo sapiens
2.4.2.8 0.011
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant mutant F36G, with hypoxanthine Homo sapiens
2.4.2.8 0.027
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant mutant F36L, with hypoxanthine Homo sapiens
2.4.2.8 0.029
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant mutant F36K, with guanine Homo sapiens
2.4.2.8 0.03
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant wild-type enzyme, with hypoxanthine Plasmodium falciparum
2.4.2.8 0.035
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant wild-type enzyme, with hypoxanthine Homo sapiens
2.4.2.8 0.041
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant mutant F36L, with guanine Homo sapiens
2.4.2.8 0.05
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant mutant F36G, with guanine Homo sapiens
2.4.2.8 0.076
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant wild-type enzyme, with guanine Homo sapiens
2.4.2.8 0.079
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant wild-type enzyme, with xanthine Plasmodium falciparum
2.4.2.8 0.102
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant mutant L44F, with hypoxanthine Plasmodium falciparum
2.4.2.8 0.116
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant mutant L44F, with guanine Plasmodium falciparum
2.4.2.8 0.151
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant mutant L44F, with xanthine Plasmodium falciparum
2.4.2.8 0.201
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 22°C, recombinant wild-type enzyme, with guanine Plasmodium falciparum
2.4.2.8 0.261
-
 xanthine pH 7.4, 22°C, recombinant wild-type enzyme Plasmodium falciparum
2.4.2.8 0.287
-
 xanthine pH 7.4, 22°C, recombinant mutant F36L Homo sapiens
2.4.2.8 0.853
-
 xanthine pH 7.4, 22°C, recombinant mutant L44F Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.8 GMP + diphosphate Homo sapiens
-
 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
-
 GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
-
 IMP + diphosphate
-
 ?
2.4.2.8 IMP + diphosphate Homo sapiens
-
 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum wild-type enzyme xanthosine 5'-phosphate + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate Homo sapiens mutant F36L, no activity with the wild-type enzyme xanthosine 5'-phosphate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Homo sapiens P00492
-
-
2.4.2.8 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8 recombinant wild-type and mutant L44F from Escherichia coli Plasmodium falciparum
2.4.2.8 recombinant wild-type and mutants F36L, F36G, F36K from Escherichia coli Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.8 IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate residue F36 is responsible for substrate specificity, structure-function relationship Homo sapiens
a
2.4.2.8 IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate residue L44 is responsible for substrate specificity, structure-function relationship Plasmodium falciparum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 GMP + diphosphate
-
 Homo sapiens guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Plasmodium falciparum GMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate wild-type enzyme, no activity with mutant F36L Homo sapiens GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Plasmodium falciparum IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate wild-type enzyme, no activity with mutant F36L Homo sapiens IMP + diphosphate
-
 ?
2.4.2.8 IMP + diphosphate
-
 Homo sapiens hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.8 additional information the flexibilty of loop IV can influence the substrate specificity Homo sapiens ?
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate wild-type enzyme Plasmodium falciparum xanthosine 5'-phosphate + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate mutant F36L, no activity with the wild-type enzyme Homo sapiens xanthosine 5'-phosphate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.8 HGPRT
-
 Plasmodium falciparum
2.4.2.8 HGPRT
-
 Homo sapiens
2.4.2.8 hypoxanthine guanine phosphoribosyltransferase
-
 Plasmodium falciparum
2.4.2.8 hypoxanthine guanine phosphoribosyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.8 22
-
 assay at room temperature Plasmodium falciparum
2.4.2.8 22
-
 assay at room temperature Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.8 60
-
 3 h, wild-type and mutant F36L, over 80% remaining activity Homo sapiens
2.4.2.8 70
-
 0.5 h, wild-type and mutant F36L, over 80% remaining activity Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.8 0.07
-
 xanthine pH 7.4, 22°C, recombinant mutant F36L Homo sapiens
2.4.2.8 0.94
-
 hypoxanthine pH 7.4, 22°C, recombinant mutant L44F Plasmodium falciparum
2.4.2.8 1.1
-
 guanine pH 7.4, 22°C, recombinant mutant L44F Plasmodium falciparum
2.4.2.8 1.13
-
 hypoxanthine pH 7.4, 22°C, recombinant wild-type enzyme Plasmodium falciparum
2.4.2.8 1.2
-
 xanthine pH 7.4, 22°C, recombinant mutant L44F Plasmodium falciparum
2.4.2.8 1.2
-
 guanine pH 7.4, 22°C, recombinant wild-type enzyme Plasmodium falciparum
2.4.2.8 2.9
-
 xanthine pH 7.4, 22°C, recombinant wild-type enzyme Plasmodium falciparum
2.4.2.8 3.1
-
 hypoxanthine pH 7.4, 22°C, recombinant mutant F36G Homo sapiens
2.4.2.8 3.4
-
 guanine pH 7.4, 22°C, recombinant mutant F36G Homo sapiens
2.4.2.8 3.4
-
 hypoxanthine pH 7.4, 22°C, recombinant mutant F36K Homo sapiens
2.4.2.8 5.4
-
 hypoxanthine pH 7.4, 22°C, recombinant mutant F36L Homo sapiens
2.4.2.8 6.3
-
 guanine pH 7.4, 22°C, recombinant mutant F36K Homo sapiens
2.4.2.8 7.1
-
 hypoxanthine pH 7.4, 22°C, recombinant wild-type enzyme Homo sapiens
2.4.2.8 18
-
 guanine pH 7.4, 22°C, recombinant mutant F36L Homo sapiens
2.4.2.8 25.5
-
 guanine pH 7.4, 22°C, recombinant wild-type enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.8 7.4
-
 assay at Plasmodium falciparum
2.4.2.8 7.4
-
 assay at Homo sapiens