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Literature summary extracted from

  • Diaz-Enrich, M.J.; Ibarguren, I.; Hellman, U.; Villamarin, J.A.
    Characterization of a type I regulatory subunit of cAMP-dependent protein kinase from the bivalve mollusk Mytilus galloprovincialis (2003), Arch. Biochem. Biophys., 416, 119-127.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.11 cAMP dependent on Mytilus galloprovincialis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.11 Mg2+
-
 Mytilus galloprovincialis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.11 54000
-
 1 * 54000, Rmyt2, reducing or nonreducing SDS-PAGE, 1 * 54000, Rmyt1, reducing SDS-PAGE Mytilus galloprovincialis
2.7.11.11 54000
-
 2 * 54000, (Rmyt1)2, nonreducing SDS-PAGE Mytilus galloprovincialis

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Mytilus galloprovincialis
-
 bivalve mollusk, mussel
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.11 purification of the type 1 regulatory subunit Rmyt1 from mussel by DEAE ion exchange and immunoaffinity chromatography, the latter based on antibodies and cGMP-agarose, and gel filtration Mytilus galloprovincialis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 ATP + Kemptide activity of the catalytic subunit C Mytilus galloprovincialis ADP + Kemptide phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.11 dimer 2 * 54000, (Rmyt1)2, nonreducing SDS-PAGE Mytilus galloprovincialis
2.7.11.11 monomer 1 * 54000, Rmyt2, reducing or nonreducing SDS-PAGE, 1 * 54000, Rmyt1, reducing SDS-PAGE Mytilus galloprovincialis
2.7.11.11 More 2 isoforms of regulatory subunit termed Rmyt1, type 1, and Rmyt2, one form of catalytic subunit C, peptide mapping of Rmyt1 Mytilus galloprovincialis

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
-
 Mytilus galloprovincialis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.11 22
-
 assay at room temperature Mytilus galloprovincialis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.11 7
-
 assay at Mytilus galloprovincialis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP
-
 Mytilus galloprovincialis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.11.11 Mytilus galloprovincialis regulatory subunit Rmyt2, isoelectric focusing
-
 4.7
2.7.11.11 Mytilus galloprovincialis regulatory subunit Rmyt1, isoelectric focusing
-
 5