Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Silva, E.; Marques, A.R.; Fialho, A.M.; Granja, A.T.; Sa-Correia, I.
    Proteins encoded by Sphingomonas elodea ATCC 31461 rmlA and ugpG genes, involved in gellan gum biosynthesis, exhibit both dTDP- and UDP-glucose pyrophosphorylase activities (2005), Appl. Environ. Microbiol., 71, 4703-4712.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.9 expression in Escherichia coli Sphingomonas elodea
2.7.7.24 expression in Escherichia coli, RmlA protein Sphingomonas elodea
2.7.7.24 expression in Escherichia coli, UgpG protein Sphingomonas elodea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 0.0075
-
UTP isoenzyme UgpG, 30°C, pH 7.8 Sphingomonas elodea
2.7.7.9 0.229
-
UTP isoenzyme RmlA, 30°C, pH 7.8 Sphingomonas elodea
2.7.7.24 0.0094
-
dTDP-glucose UgpG protein Sphingomonas elodea
2.7.7.24 0.012
-
dTDP-glucose RmlA protein Sphingomonas elodea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.9 31500
-
SDS-PAGE Sphingomonas elodea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.24 dTDP-glucose + diphosphate Sphingomonas elodea protein by ugpG gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities dTTP + alpha-D-glucose 1-phosphate
-
?
2.7.7.24 dTDP-glucose + diphosphate Sphingomonas elodea protein encoded by rmlA gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities dTTP + alpha-D-glucose 1-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Sphingomonas elodea
-
ATCC 31461
-
2.7.7.24 Sphingomonas elodea
-
ATCC 31461
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9
-
Sphingomonas elodea
2.7.7.24 recombinant enzyme, RmlA protein Sphingomonas elodea
2.7.7.24 recombinant enzyme, UgpG protein Sphingomonas elodea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.9 additional information
-
Vmax isoenzyme RmlA 37.05, 30°C, pH 7.8 Sphingomonas elodea
2.7.7.9 additional information
-
Vmax isoenzyme UgpG 1.28, 30°C, pH 7.8 Sphingomonas elodea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 UTP + D-glucose-1-phosphate
-
Sphingomonas elodea UDP-glucose + diphosphate
-
?
2.7.7.24 dTDP-glucose + diphosphate
-
Sphingomonas elodea dTTP + alpha-D-glucose 1-phosphate
-
?
2.7.7.24 dTDP-glucose + diphosphate protein by ugpG gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities Sphingomonas elodea dTTP + alpha-D-glucose 1-phosphate
-
?
2.7.7.24 dTDP-glucose + diphosphate protein encoded by rmlA gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities Sphingomonas elodea dTTP + alpha-D-glucose 1-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.24 RmlA
-
Sphingomonas elodea
2.7.7.24 RmlA protein protein encoded by rmlA gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities Sphingomonas elodea
2.7.7.24 ugpG
-
Sphingomonas elodea
2.7.7.24 UgpG protein protein encoded by ugpG gene is involved in gellan gum biosynthesis and exhibits both dTDP- and UDP-glucose diphosphorylase activities Sphingomonas elodea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.24 0.56
-
dTDP-glucose UgpG protein Sphingomonas elodea
2.7.7.24 1.35
-
dTDP-glucose RmlA protein Sphingomonas elodea